CAJ | 학술논문

目的：了解急性乙型肝炎（A HB ）患者外周血 T h17细胞频率及相关细胞因子 IL‐21、IL‐22和IL‐23在病程各期的表达水平。方法流式细胞术检测10例A HB患者急性期、恢复早期和痊愈后外周血Th17细胞频率，ELISA检测IL‐21、IL‐22及IL‐23的水平。健康体检者10名作为对照。两组间比较采用 t检验，多组间比较采用单因素方差分析，相关性检验采用Pearson相关分析。结果健康对照组外周血 T h17细胞频率为（0．68±0．29）％，A HB患者急性期、恢复早期和痊愈后分别为（18．22±4．13）、（3．14±1．90）和（3．31±0．95）％，与健康对照组比较，差异有统计学意义（t值分别为13．405、4．047和8．342，均 P＜0．01）。健康对照组 IL‐21、IL‐22、IL‐23分别为（42．00±6．95）、（315．89±96．16）和（11．95±6．95） ng／L ，AHB患者急性期分别为（575．39±47．01）、（648．44±47．12）和（38．29±4．68） ng／L ，恢复早期分别为（366．50±33．74）、（405．04±47．12）和（25．10±4．69） ng／L ，痊愈后分别为（46．62±8．28）、（365．94±45．62）和（15．29±4．69） ng／L。AHB患者各期与健康对照组相比，IL‐21（t值分别为35．497、29．792和1．354，P值分别为＜0．01、＜0．01和0．193）、IL‐22（t值分别为9．820、2．632、1．487，P值分别为＜0．01、0．021和0．161）和IL‐23（t值分别为9．944、4．961和1．260， P值分别为＜0．01、＜0．01和0．226）差异均有统计学意义；3种细胞因子各期比较差异均有统计学意义（ F值分别为622．784、107．772和60．743，均 P＜0．01）。患者急性期 IL‐21、IL‐22、IL‐23水平与血清A L T水平呈正相关关系（ r值分别为0．655、0．666和0．673，均 P＜0．05）。相关性分析发现，T h17与IL‐21、IL‐22和IL‐23水平在急性期均呈正相关关系（ r值分别为0．879、0．866和0．879，均 P＜0．01）， Th17与IL‐21痊愈后呈正相关关系（r＝0．641，P＝0．046），余均无相关性。结论 Th17及相关细胞因子IL‐21、IL‐22、IL‐23的表达水平随着A HB病情好转呈下降趋势。目的：瞭解急性乙型肝炎（A HB ）患者外週血 T h17細胞頻率及相關細胞因子 IL‐21、IL‐22和IL‐23在病程各期的錶達水平。方法流式細胞術檢測10例A HB患者急性期、恢複早期和痊愈後外週血Th17細胞頻率，ELISA檢測IL‐21、IL‐22及IL‐23的水平。健康體檢者10名作為對照。兩組間比較採用 t檢驗，多組間比較採用單因素方差分析，相關性檢驗採用Pearson相關分析。結果健康對照組外週血 T h17細胞頻率為（0．68±0．29）％，A HB患者急性期、恢複早期和痊愈後分彆為（18．22±4．13）、（3．14±1．90）和（3．31±0．95）％，與健康對照組比較，差異有統計學意義（t值分彆為13．405、4．047和8．342，均 P＜0．01）。健康對照組 IL‐21、IL‐22、IL‐23分彆為（42．00±6．95）、（315．89±96．16）和（11．95±6．95） ng／L ，AHB患者急性期分彆為（575．39±47．01）、（648．44±47．12）和（38．29±4．68） ng／L ，恢複早期分彆為（366．50±33．74）、（405．04±47．12）和（25．10±4．69） ng／L ，痊愈後分彆為（46．62±8．28）、（365．94±45．62）和（15．29±4．69） ng／L。AHB患者各期與健康對照組相比，IL‐21（t值分彆為35．497、29．792和1．354，P值分彆為＜0．01、＜0．01和0．193）、IL‐22（t值分彆為9．820、2．632、1．487，P值分彆為＜0．01、0．021和0．161）和IL‐23（t值分彆為9．944、4．961和1．260， P值分彆為＜0．01、＜0．01和0．226）差異均有統計學意義；3種細胞因子各期比較差異均有統計學意義（ F值分彆為622．784、107．772和60．743，均 P＜0．01）。患者急性期 IL‐21、IL‐22、IL‐23水平與血清A L T水平呈正相關關繫（ r值分彆為0．655、0．666和0．673，均 P＜0．05）。相關性分析髮現，T h17與IL‐21、IL‐22和IL‐23水平在急性期均呈正相關關繫（ r值分彆為0．879、0．866和0．879，均 P＜0．01）， Th17與IL‐21痊愈後呈正相關關繫（r＝0．641，P＝0．046），餘均無相關性。結論 Th17及相關細胞因子IL‐21、IL‐22、IL‐23的錶達水平隨著A HB病情好轉呈下降趨勢。
목적：료해급성을형간염（A HB ）환자외주혈 T h17세포빈솔급상관세포인자 IL‐21、IL‐22화IL‐23재병정각기적표체수평。방법류식세포술검측10례A HB환자급성기、회복조기화전유후외주혈Th17세포빈솔，ELISA검측IL‐21、IL‐22급IL‐23적수평。건강체검자10명작위대조。량조간비교채용 t검험，다조간비교채용단인소방차분석，상관성검험채용Pearson상관분석。결과건강대조조외주혈 T h17세포빈솔위（0．68±0．29）％，A HB환자급성기、회복조기화전유후분별위（18．22±4．13）、（3．14±1．90）화（3．31±0．95）％，여건강대조조비교，차이유통계학의의（t치분별위13．405、4．047화8．342，균 P＜0．01）。건강대조조 IL‐21、IL‐22、IL‐23분별위（42．00±6．95）、（315．89±96．16）화（11．95±6．95） ng／L ，AHB환자급성기분별위（575．39±47．01）、（648．44±47．12）화（38．29±4．68） ng／L ，회복조기분별위（366．50±33．74）、（405．04±47．12）화（25．10±4．69） ng／L ，전유후분별위（46．62±8．28）、（365．94±45．62）화（15．29±4．69） ng／L。AHB환자각기여건강대조조상비，IL‐21（t치분별위35．497、29．792화1．354，P치분별위＜0．01、＜0．01화0．193）、IL‐22（t치분별위9．820、2．632、1．487，P치분별위＜0．01、0．021화0．161）화IL‐23（t치분별위9．944、4．961화1．260， P치분별위＜0．01、＜0．01화0．226）차이균유통계학의의；3충세포인자각기비교차이균유통계학의의（ F치분별위622．784、107．772화60．743，균 P＜0．01）。환자급성기 IL‐21、IL‐22、IL‐23수평여혈청A L T수평정정상관관계（ r치분별위0．655、0．666화0．673，균 P＜0．05）。상관성분석발현，T h17여IL‐21、IL‐22화IL‐23수평재급성기균정정상관관계（ r치분별위0．879、0．866화0．879，균 P＜0．01）， Th17여IL‐21전유후정정상관관계（r＝0．641，P＝0．046），여균무상관성。결론 Th17급상관세포인자IL‐21、IL‐22、IL‐23적표체수평수착A HB병정호전정하강추세。
Objective To investigate the expression of T helper type 17 cells (Th17) and cell‐related cytokines ,including interleukin (IL)‐21 ,IL‐22 ,IL‐23 in the peripheral blood of different clinical stages of patients with acute hepatitis B (AHB) .Methods Ten cases of AHB patients were enrolled .The frequency of Th17 cells in the three clinical stages (i .e .acute phase ,convalescent phase and resolved phase) were detected by flow cytometry . IL‐21 , IL‐22 and IL‐23 were measured by enzyme‐linked immunosorbent assay (ELISA) .Control group was composed of ten healthy subjects .The comparison between the two groups was done by t test and the differences among multiple groups were compared by one way ANOVA .Pearson correlation analysis was used for correlation analysis .Results The frequency of Th17 in healthy controls was (0 .68 ± 0 .29)% ,while those in acute phase ,convalescent phase and resolved phase of AHB patients were (18 .22 ± 4 .13)% , (3 .14 ± 1 .90 )% and (3 .31 ± 0 .95 )% , The differences between the two groups were significant (t= 13 .405 ,4 .047 and 8 .342 , respectively ;all P< 0 .01) .The levels of IL‐21 ,IL‐22 and IL‐23 in healthy controls were (42 .00 ± 6 .95) ,(315 .89 ± 96 .16) and (11 .95 ± 6 .95) ng/L ,respectively .Those in acute phase of AHB patients were (575 .39 ± 47 .01) ,(648 .44 ± 47 .12) and (38 .29 ± 4 .68) ng/L ,respectively ,those in convalescent phase were (366 .50 ± 33 .74) ,(405 .04 ± 47 .12) and (25 .10 ± 4 .69) ng/L ,respectively ,while those in resolved phase of AHB patients were (46 .62 ± 8 .28) ,(365 .94 ± 45 .62) and (15 .29 ± 4 .69) ng/L , respectively .Compared with healthy controls ,t values of the levels of IL‐21 in three different phases of AHB patients were 35 .497 ,29 .792 and 1 .354 with P value of <0 .01 ,<0 .01 and 0 .193 ,respectively ;those of IL‐22 were 9 .820 ,2 .632 and 1 .487 with P value of < 0 .01 ,0 .021 and 0 .161 ,respectively ;those of IL‐23 were 9 .944 ,4 .961 and 1 .260 with P values of <0 .01 ,<0 .01 and 0 .226 ,respectively . After comparison of IL‐21 ,IL‐22 and IL‐23 among three different phase of AHB ,F values were 622 .784 , 107 .772 and 60 .743 with all P values less than 0 .01 ,respectively .The levels of IL‐21 ,IL‐22 and IL‐23 were all positively correlated with the serum ALT level in acute phase (r= 0 .655 ,0 .666 and 0 .673 , respectively ;all P<0 .05) .Correlation analysis demonstrated that the frequency of Th17 was positively correlated with the levels of IL‐21 , IL‐22 and IL‐23 in acute phase ( r= 0 .879 ,0 .866 and 0 .879 , respectively ;all P<0 .01) .The frequency of Th17 was positively correlated with the level of IL‐21 in the resolved phase . No correlations between the remaining groups were confirmed . Conclusion The expressions of Th17 and cell‐related cytokines ,including IL‐21 ,IL‐22 and IL‐23 decline with the recovery of A HB .