CAJ | 학술논문

背景：研究表明，人端粒酶反转录酶(human telomerase reverse transcriptase, hTERT)具有加强细胞增殖能力、保持端粒长度恒定、延长体外培养细胞寿命等作用。目的：观察hTERT基因转染骨髓间充质干细胞移植对脑梗死大鼠神经功能恢复的影响。方法：建立大鼠大脑中动脉阻塞模型，随机分为脑梗死组、骨髓间充质干细胞移植组和hTERT转染骨髓间充质干细胞移植组，每组20只，分别于尾静脉注射1 mL PBS，1 mL第9代骨髓间充质干细胞悬液(细胞浓度2.5×107 L-1)和第9代hTERT基因转染骨髓间充质干细胞悬液(细胞浓度2.5×107 L-1)。于移植前及移植后对各组大鼠进行改良神经功能学评分，应用RT-PCR、Western Blot检测梗死脑组织中hTERT基因及蛋白表达的变化，TUNEL法测定脑组织中细胞凋亡情况。结果与结论：hTERT基因转染骨髓间充质干细胞的细胞生长周期明显延长，随着传代次数的增加，细胞生长良好，无明显形态改变；hTERT转染骨髓间充质干细胞移植组的hTERT基因及蛋白表达明显优于骨髓间充质干细移植组，差异有显著性意义(P <0.05)；与脑梗死组及骨髓间充质干细胞移植组比较，hTERT转染骨髓间充质干细胞移植组的神经功能缺损评分明显降低，脑组织中凋亡细胞数目明显减少(P <0.05)。结果显示hTERT基因转染骨髓间充质干细胞能促进脑梗死大鼠神经功能恢复。
배경：연구표명，인단립매반전록매(human telomerase reverse transcriptase, hTERT)구유가강세포증식능력、보지단립장도항정、연장체외배양세포수명등작용。목적：관찰hTERT기인전염골수간충질간세포이식대뇌경사대서신경공능회복적영향。방법：건립대서대뇌중동맥조새모형，수궤분위뇌경사조、골수간충질간세포이식조화hTERT전염골수간충질간세포이식조，매조20지，분별우미정맥주사1 mL PBS，1 mL제9대골수간충질간세포현액(세포농도2.5×107 L-1)화제9대hTERT기인전염골수간충질간세포현액(세포농도2.5×107 L-1)。우이식전급이식후대각조대서진행개량신경공능학평분，응용RT-PCR、Western Blot검측경사뇌조직중hTERT기인급단백표체적변화，TUNEL법측정뇌조직중세포조망정황。결과여결론：hTERT기인전염골수간충질간세포적세포생장주기명현연장，수착전대차수적증가，세포생장량호，무명현형태개변；hTERT전염골수간충질간세포이식조적hTERT기인급단백표체명현우우골수간충질간세이식조，차이유현저성의의(P <0.05)；여뇌경사조급골수간충질간세포이식조비교，hTERT전염골수간충질간세포이식조적신경공능결손평분명현강저，뇌조직중조망세포수목명현감소(P <0.05)。결과현시hTERT기인전염골수간충질간세포능촉진뇌경사대서신경공능회복。
BACKGROUND:Studies have shown that human telomerase reverse transcriptase (hTERT) has the ability to enhance cel proliferation, maintain telomere length, prolonged cel life cultured in vitro. OBJECTIVE:To observe the effect of hTERT gene-modified bone marrow mesechymal stem cel transplantation on neural function recovery of rats with cerebral infarction. METHODS:Rat models of middle cerebral artery occlusion were established and randomized into model group, cel transplantation group and hTERT-modified cel transplantation group, with 20 rats in each group. Rats in the three groups were respectively injected via tail vein with 1 mL PBS, passage 9 bone marrow mesenchymal stem cel suspension (2.5×107/L) and hTERT-modified passage 9 bone marrow mesenchymal stem cel suspension (2.5×107/L), respectively. Modified neurological severity scores were determined before and after transplantation; RT-PCR and western blot assay were used to measure hTERT expression at gene and protein levels; TUNEL method was adopted to detect cel apoptosis in the brain. RESULTS AND CONCLUSION:hTERT-modified bone marrow mesenchymal stem cels had prolonged cel cycle, and with the increase in passage number, the cels showed good growth with no changes in morphology. The expressions of hTERT mRNA and protein were superior in the hTERT-modified cel transplantation group than the cel transplantation group, and there was a significant difference (P < 0.05). Modified neurological severity scores and number of apoptotic cels were decreased significantly in the hTERT-modified cel transplantation group compared with the other two groups (P < 0.05). These findings indicate that hTERT-modified bone marrow mesenchymal stem cels can promote neural functional recovery of rats with cerebral infarction.