CAJ | 학술논문

背景：研究发现，骨髓间充质干细胞具有修复神经元的作用，但单纯骨髓间充质干细胞移植对神经系统损伤的修复作用仍不理想。目的：探讨骨髓间充质干细胞移植联合丙泊酚对脊髓损伤大鼠后肢功能和电生理的影响。方法：80只成年的Wistar大鼠，建立脊髓损伤模型，按照随机数字表法分为4组(n=20)：骨髓间充质干细胞组、对照组、联合组、丙泊酚组。建模后6 h通过1 mL注射器经尾静脉注入骨髓间充质干细胞悬液、细胞培养液、骨髓间充质干细胞悬液+丙泊酚注射液、丙泊酚注射液。分别于造模前、造模后1，3 d与1-4周通过BBB评分、改良Tarlov评分、斜板试验进行运动功能评定。造模后4周取材用荧光显微镜观测PKH-26标记的骨髓间充质干细胞存活及分布情况，病理切片进行苏木精-伊红染色。第4周进行辣根过氧化物酶示踪分析神经纤维的再生情况，运动诱发电位和体感诱发电位分析大鼠神经电生理恢复情况。结果与结论：①大鼠下肢运动功能评价联合组优于骨髓间充质干细胞组及丙泊酚组，骨髓间充质干细胞组和丙泊酚组优于对照组。②丙泊酚组和骨髓间充质干细胞组损伤区可见少量神经轴索样的结构，该脊髓空洞比较小，联合组可见较多的神经轴索样结构，未见脊髓空洞。对照组可见脊髓组织缺失及脊髓空洞形成，无神经轴索通过。③辣根过氧化物酶阳性神经纤维数和PKH-26阳性细胞：对照组<丙泊酚组、骨髓间充质干细胞组<联合组，各组之间差异均有显著性意义(P <0.05)。④运动诱发电位和体感诱发电位的潜伏期：对照组>丙泊酚组、骨髓间充质干细胞组>联合组，各组之间差异均有显著性意义(P <0.05)。⑤运动诱发电位和体感诱发电位的波幅：对照组<丙泊酚组与骨髓间充质干细胞组<联合组，各组之间差异均有显著性意义(P <0.05)。⑥结果表明丙泊酚、骨髓间充质干细胞均能促进脊髓损伤大鼠神经突触的再生，改善大鼠电生理功能及肢体运动功能，二者联用效果更好。揹景：研究髮現，骨髓間充質榦細胞具有脩複神經元的作用，但單純骨髓間充質榦細胞移植對神經繫統損傷的脩複作用仍不理想。目的：探討骨髓間充質榦細胞移植聯閤丙泊酚對脊髓損傷大鼠後肢功能和電生理的影響。方法：80隻成年的Wistar大鼠，建立脊髓損傷模型，按照隨機數字錶法分為4組(n=20)：骨髓間充質榦細胞組、對照組、聯閤組、丙泊酚組。建模後6 h通過1 mL註射器經尾靜脈註入骨髓間充質榦細胞懸液、細胞培養液、骨髓間充質榦細胞懸液+丙泊酚註射液、丙泊酚註射液。分彆于造模前、造模後1，3 d與1-4週通過BBB評分、改良Tarlov評分、斜闆試驗進行運動功能評定。造模後4週取材用熒光顯微鏡觀測PKH-26標記的骨髓間充質榦細胞存活及分佈情況，病理切片進行囌木精-伊紅染色。第4週進行辣根過氧化物酶示蹤分析神經纖維的再生情況，運動誘髮電位和體感誘髮電位分析大鼠神經電生理恢複情況。結果與結論：①大鼠下肢運動功能評價聯閤組優于骨髓間充質榦細胞組及丙泊酚組，骨髓間充質榦細胞組和丙泊酚組優于對照組。②丙泊酚組和骨髓間充質榦細胞組損傷區可見少量神經軸索樣的結構，該脊髓空洞比較小，聯閤組可見較多的神經軸索樣結構，未見脊髓空洞。對照組可見脊髓組織缺失及脊髓空洞形成，無神經軸索通過。③辣根過氧化物酶暘性神經纖維數和PKH-26暘性細胞：對照組<丙泊酚組、骨髓間充質榦細胞組<聯閤組，各組之間差異均有顯著性意義(P <0.05)。④運動誘髮電位和體感誘髮電位的潛伏期：對照組>丙泊酚組、骨髓間充質榦細胞組>聯閤組，各組之間差異均有顯著性意義(P <0.05)。⑤運動誘髮電位和體感誘髮電位的波幅：對照組<丙泊酚組與骨髓間充質榦細胞組<聯閤組，各組之間差異均有顯著性意義(P <0.05)。⑥結果錶明丙泊酚、骨髓間充質榦細胞均能促進脊髓損傷大鼠神經突觸的再生，改善大鼠電生理功能及肢體運動功能，二者聯用效果更好。
배경：연구발현，골수간충질간세포구유수복신경원적작용，단단순골수간충질간세포이식대신경계통손상적수복작용잉불이상。목적：탐토골수간충질간세포이식연합병박분대척수손상대서후지공능화전생리적영향。방법：80지성년적Wistar대서，건립척수손상모형，안조수궤수자표법분위4조(n=20)：골수간충질간세포조、대조조、연합조、병박분조。건모후6 h통과1 mL주사기경미정맥주입골수간충질간세포현액、세포배양액、골수간충질간세포현액+병박분주사액、병박분주사액。분별우조모전、조모후1，3 d여1-4주통과BBB평분、개량Tarlov평분、사판시험진행운동공능평정。조모후4주취재용형광현미경관측PKH-26표기적골수간충질간세포존활급분포정황，병리절편진행소목정-이홍염색。제4주진행랄근과양화물매시종분석신경섬유적재생정황，운동유발전위화체감유발전위분석대서신경전생리회복정황。결과여결론：①대서하지운동공능평개연합조우우골수간충질간세포조급병박분조，골수간충질간세포조화병박분조우우대조조。②병박분조화골수간충질간세포조손상구가견소량신경축색양적결구，해척수공동비교소，연합조가견교다적신경축색양결구，미견척수공동。대조조가견척수조직결실급척수공동형성，무신경축색통과。③랄근과양화물매양성신경섬유수화PKH-26양성세포：대조조<병박분조、골수간충질간세포조<연합조，각조지간차이균유현저성의의(P <0.05)。④운동유발전위화체감유발전위적잠복기：대조조>병박분조、골수간충질간세포조>연합조，각조지간차이균유현저성의의(P <0.05)。⑤운동유발전위화체감유발전위적파폭：대조조<병박분조여골수간충질간세포조<연합조，각조지간차이균유현저성의의(P <0.05)。⑥결과표명병박분、골수간충질간세포균능촉진척수손상대서신경돌촉적재생，개선대서전생리공능급지체운동공능，이자련용효과경호。
BACKGROUND:Bone marrow mesenchymal stem cels can be used to repair neurons, but have no ideal outcomes on nervous system injuries. OBJECTIVE:To investigate the effects of bone marrow mesenchymal stem cel transplantation combined with propofol on the hind limb function and electrophysiological changes of rats with spinal cord injury. METHODS:Eighty adult Wistar rats were selected to make animal models of spinal cord injury, and then randomized into four groups (n=20): bone marrow mesenchymal stem cel group, control group, combination group, propofol group. At 6 hours after modeling, rats in these four groups were injectedvia the tail vein with bone marrow mesenchymal stem cel suspension, cel culture medium, bone marrow mesenchymal stem cel suspension+propofol solution, and propofol solution using a 1 mL syringe, respectively. Rat motor function was assessed by Basso Beattie Bresnahan score, modified Tarlov score and inclined plane test before and at 1 day, 3 days, 1-4 weeks after modeling. Under fluorescence microscope, the survival and distribution of PKH-26-labeled bone marrow mesenchymal stem cels were observed at 4 weeks after modeling, and meanwhile, hematoxylin-eosin staining was used for pathological observation. Horseradish peroxidase tracer analysis was performed to analyze regeneration of nerve fibers, and motor and somatosensory evoked potentials were used to analyze the neurophysiological recovery of rats. RESULTS AND CONCLUSION:(1) The motor function of the rat hind limb recovered best in the combination group, better in the bone marrow mesenchymal stem cel group and propofol group, but worse in the control group. (2) There were a smal amount of nerve axon-like structures and smal syringomyelia in the bone marrow mesenchymal stem cel group and propofol group, but the combination group had more axon-like structures and no syringomyelia. In the control group, no axons but spinal cord defects and syringomyelia formed. (3) The amount of horseradish peroxidase-positive nerve fibers and the number of PKH-26 positive cels were ranked as folows: control group < propofol group and bone marrow mesenchymal stem cel group < combination group. There were significant differences between the groups (P < 0.05). (4) The latencies of motor and somatosensory evoked potentials were ranked as folows: control group> propofol group and bone marrow mesenchymal stem cel group > combination group, and there were significant differences between the groups (P < 0.05). (5) Amplitudes of motor and somatosensory evoked potentials were arranged as folows: control group < propofol group and bone marrow mesenchymal stem cel group < combination group, and the differences were statisticaly significant (P < 0.05). These findings indicate that both propofol and bone marrow mesenchymal stem cels can promote synaptic regeneration and improve the electrophysiological function and motor function of rats with spinal cord injury. Their combination has a better role than propofol and bone marrow mesenchymal stem cels used alone.