CAJ | 학술논문

背景：CT灌注技术是目前临床上较为常用的无创检查方法，可以早期定量检测到脑梗死组织的缺血程度及范围，进而判断脑组织成活与否及其可恢复性。目的：应用CT灌注技术评价神经干细胞移植脑梗死大鼠的神经功能恢复情况。方法：60只SD大鼠中随机分为对照组、脑梗死组、移植组，每组20只，后两组制备大脑中动脉梗死模型，建模后24 h，脑梗死组通过尾静脉注射PBS，移植组注射8×105个神经干细胞。分别于细胞移植后的1，3，7，14，28 d行CT灌注成像，移植后1，2，3，4周采用mNSS进行神经功能评分，移植后4周TTC染色计算各组的脑梗死体积，移植后2周苏木精-伊红染色观察脑组织病理变化。结果与结论：对照组在各个时间点血流动力学未见明显异常，移植组的 CT 值随时间变化逐渐升高，脑血流量的升高可以增加缺血半暗带神经细胞的存活率。移植组的神经功能评分低于脑梗死组，梗死体积小于脑梗死组(P <0.05)，移植组梗死灶细胞变性坏死程度明显减轻。结果表明CT灌注成像能够从形态学及血流动力学方面观察神经干细胞移植脑梗死大鼠的神经功能恢复情况。
배경：CT관주기술시목전림상상교위상용적무창검사방법，가이조기정량검측도뇌경사조직적결혈정도급범위，진이판단뇌조직성활여부급기가회복성。목적：응용CT관주기술평개신경간세포이식뇌경사대서적신경공능회복정황。방법：60지SD대서중수궤분위대조조、뇌경사조、이식조，매조20지，후량조제비대뇌중동맥경사모형，건모후24 h，뇌경사조통과미정맥주사PBS，이식조주사8×105개신경간세포。분별우세포이식후적1，3，7，14，28 d행CT관주성상，이식후1，2，3，4주채용mNSS진행신경공능평분，이식후4주TTC염색계산각조적뇌경사체적，이식후2주소목정-이홍염색관찰뇌조직병리변화。결과여결론：대조조재각개시간점혈류동역학미견명현이상，이식조적 CT 치수시간변화축점승고，뇌혈류량적승고가이증가결혈반암대신경세포적존활솔。이식조적신경공능평분저우뇌경사조，경사체적소우뇌경사조(P <0.05)，이식조경사조세포변성배사정도명현감경。결과표명CT관주성상능구종형태학급혈류동역학방면관찰신경간세포이식뇌경사대서적신경공능회복정황。
BACKGROUND:CT perfusion technology is a common non-invasive detection method, which can be used to quantitatively determine the ischemia severity and range at early stage of cerebral infarction and then judge whether ischemic brain tissues can survive or recover. OBJECTIVE:To assess the neurological function recovery of cerebral infarction rats undergoing neural stem cel transplantation using CT perfusion imaging. METHODS:A total of 60 Sprague-Dawley rats were randomly divided into control group, cerebral infarction group, transplantation group, with 20 rats in each group. Rat model of middle cerebral artery occlusion was made in the latter two groups. After 24 hours of modeling, PBS and 8×105 neural stem cels were administratedvia the tail vein into the rats in the cerebral infarction and transplantation groups, respectively. CT perfusion-weighted imaging was performed at 1, 3, 7, 14, 28 days after transplantation. Modified neurological severity scores were recorded at 1, 2, 3, 4 weeks after transplantation. Triphenyltetrazolium chloride staining was used to calculate infarct volume at 4 weeks after transplantation. Hematoxylin- eosin staining was adopted to observe pathological changes of brain tissues at 2 weeks after transplantation. RESULTS AND CONCLUSION: There were no abnormal hemodynamic changes in the control group at different time points. The transplantation group exhibited an increasing CT value with time, and the increased cerebral blood flow could improve the survival rate of neurons in the ischemic penumbra. The modified neurological severity score and infract volume in the transplantation group were both significantly lower than those in the cerebral infarction group (P < 0.05). Cel necrosis was improved obviously in the transplantation group. These results show that CT perfusion imaging can be used to observe the neurologic function recovery of cerebral infarction rats in aspects of morphology and hemodynamics.