CAJ | 학술논문

背景：临床用于治疗血管狭窄疾病的药物洗脱支架和单纯内皮修复型支架存在内皮化延迟和植入后再狭窄的问题。雷帕霉素联合 CD34抗体复合支架可协同抵消抗增殖药物的内皮化延迟和内膜的过度增生，目前尚处于实验研究阶段。目的：观察雷帕霉素联合CD34抗体复合支架捕获内皮祖细胞能力及其所捕获内皮祖细胞的分化特征。方法：通过扫描电镜及间接免疫荧光观察雷帕霉素联合 CD34抗体复合支架体外捕获外周血内皮祖细胞形态及其分化特征。通过荧光显微镜观察雷帕霉素联合 CD34抗体复合支架植入兔耳动脉后捕获内皮祖细胞情况及支架片段的内皮化程度。结果与结论：扫描电镜观察到CD34抗体涂层支架可捕获直径6-8μm的纺锤状细胞，24 h时细胞变得充盈饱满。所捕获细胞具有内皮祖细胞的外形特征。间接免疫荧光观察 CD34抗体支架表面可见大量血管内皮生长因子受体2阳性细胞黏附的红色荧光斑点。免疫荧光观察到CD34抗体涂层支架植入兔耳动脉24 h大部分被血管内皮细胞所覆盖，48 h达到完全覆盖，未见细胞异常聚集。结果表明雷帕霉素联合CD34抗体复合支架能特异性快速捕获外周血液中的血管内皮祖细胞，植入体内48 h即可完成血管内皮细胞覆盖，实现了支架快速内皮化，可促进内皮细胞修复。
배경：림상용우치료혈관협착질병적약물세탈지가화단순내피수복형지가존재내피화연지화식입후재협착적문제。뢰파매소연합 CD34항체복합지가가협동저소항증식약물적내피화연지화내막적과도증생，목전상처우실험연구계단。목적：관찰뢰파매소연합CD34항체복합지가포획내피조세포능력급기소포획내피조세포적분화특정。방법：통과소묘전경급간접면역형광관찰뢰파매소연합 CD34항체복합지가체외포획외주혈내피조세포형태급기분화특정。통과형광현미경관찰뢰파매소연합 CD34항체복합지가식입토이동맥후포획내피조세포정황급지가편단적내피화정도。결과여결론：소묘전경관찰도CD34항체도층지가가포획직경6-8μm적방추상세포，24 h시세포변득충영포만。소포획세포구유내피조세포적외형특정。간접면역형광관찰 CD34항체지가표면가견대량혈관내피생장인자수체2양성세포점부적홍색형광반점。면역형광관찰도CD34항체도층지가식입토이동맥24 h대부분피혈관내피세포소복개，48 h체도완전복개，미견세포이상취집。결과표명뢰파매소연합CD34항체복합지가능특이성쾌속포획외주혈액중적혈관내피조세포，식입체내48 h즉가완성혈관내피세포복개，실현료지가쾌속내피화，가촉진내피세포수복。
BACKGROUND:Drug eluting stents and endothelium stents for clinical treatment of vascular stenosis can lead to delayed endothelialization and restenosis. A rapamycin eluting stent combined with CD34 antibody can play a synergistic role to offset delayed endothelialization and intimal hyperplasia due to antiproliferative drugs, but it is stil in the pilot phase. OBJECTIVE:To observe the ability of rapamycin eluting stent combined with CD34 antibody to capture endothelial progenitor cels, and to observe the differentiation characteristics of the captured cels. METHODS:Scanning electron microscope and indirect immunofluorescence were used to observe the morphology and differentiation characteristics of captured endothelial progenitor cels. Under a fluorescence microscope, we observed the captured endothelial progenitor cels and the degree of endothelialization after implantation of the rapamycin eluting stent combined with CD34 antibody into rabbit ear vein. RESULTS AND CONCLUSION:Under the scanning electron microscope, fusiform-like cels with a diameter of 6-8 μm were captured by the composite stent, and 24 hours later, the cels became ful-shaped. The captured cels had the appearance characteristics of endothelial progenitor cels. Results from indirect immunofluorescence observation showed that there were a lot of red fluorescent spots on the coating which represented adherent cels positive for vascular endothelial growth factor receptor-2; the composite stent was largely covered with vascular endothelial cels at 24 hours after stent implantation, and fuly covered at 48 hours, but there was no abnormal cel cluster. These findings indicate that the rapamycin eluting stent combined with CD34 antibody can be specific to rapidly capture endothelial progenitor cels in the peripheral blood, and the stent can be completely covered with vascular endothelial cels at 48 hours after stent implantation, thereby achieving rapid endothelialization and promoting the repair of endothelial cels.