中国中西医结合皮肤性病学杂志
中國中西醫結閤皮膚性病學雜誌
중국중서의결합피부성병학잡지
Chinese Journal of Dermatovenerology of Integrated Traditional and Western Medicine
2015年
5期
282-284
,共3页
5-氨基酮戊酸%光动力疗法%痤疮丙酸杆菌%抑制%体外
5-氨基酮戊痠%光動力療法%痤瘡丙痠桿菌%抑製%體外
5-안기동무산%광동력요법%좌창병산간균%억제%체외
5-aminolevulinic acid%Photodynamic therapy%Propionibacterium acnes%Inhibiton%In vitro
目的 评估5-氨基酮戊酸光动力疗法(ALA-PDT)在体外对痤疮丙酸杆菌的抑制效应. 方法 试验分为ALA-PDT组、ALA组、细菌组、细菌红光组,使用半导体固态冷光源照射,采用厌氧盒培养法与倾注培养法培养,观察2.5 g/L、3.6 g/L、5.0 g/L等ALA-PDT组照光后,培养琼脂中痤疮丙酸杆菌生长情况. 结果 5.0 g/L ALA-PDT组已可完全抑制各浓度痤疮丙酸杆菌的生长,2.5 g/L ALA-PDT组各浓度痤疮丙酸杆菌可继续生长,3.6 g/L ALA-PDT组除107 CFU/mL浓度组细菌外,其余浓度组痤疮丙酸杆菌的生长已被完全抑制. 结论 同一细菌浓度下,ALA的抑菌作用与其浓度呈正相关.
目的 評估5-氨基酮戊痠光動力療法(ALA-PDT)在體外對痤瘡丙痠桿菌的抑製效應. 方法 試驗分為ALA-PDT組、ALA組、細菌組、細菌紅光組,使用半導體固態冷光源照射,採用厭氧盒培養法與傾註培養法培養,觀察2.5 g/L、3.6 g/L、5.0 g/L等ALA-PDT組照光後,培養瓊脂中痤瘡丙痠桿菌生長情況. 結果 5.0 g/L ALA-PDT組已可完全抑製各濃度痤瘡丙痠桿菌的生長,2.5 g/L ALA-PDT組各濃度痤瘡丙痠桿菌可繼續生長,3.6 g/L ALA-PDT組除107 CFU/mL濃度組細菌外,其餘濃度組痤瘡丙痠桿菌的生長已被完全抑製. 結論 同一細菌濃度下,ALA的抑菌作用與其濃度呈正相關.
목적 평고5-안기동무산광동력요법(ALA-PDT)재체외대좌창병산간균적억제효응. 방법 시험분위ALA-PDT조、ALA조、세균조、세균홍광조,사용반도체고태랭광원조사,채용염양합배양법여경주배양법배양,관찰2.5 g/L、3.6 g/L、5.0 g/L등ALA-PDT조조광후,배양경지중좌창병산간균생장정황. 결과 5.0 g/L ALA-PDT조이가완전억제각농도좌창병산간균적생장,2.5 g/L ALA-PDT조각농도좌창병산간균가계속생장,3.6 g/L ALA-PDT조제107 CFU/mL농도조세균외,기여농도조좌창병산간균적생장이피완전억제. 결론 동일세균농도하,ALA적억균작용여기농도정정상관.
Objective To evaluate the inhibitory effect of 5-aminolevulinic acid photodynamic therapy (ALA-PDT) on Propionibacterium acnes (P.acnes). Methods The experiments were divided into four groups:ALA-PDT group, ALA group, bacterium group and bacterium phototherapy group. P.acnes were irradiated by the light source of semiconductor and cultivated by anaerobic culture and poured-plate methods. The growth conditions of P.acnes were observed after ALA-PDT at the ALA concentrations of 2.5 g/L, 3.6 g/L and 5 g/L respectively. Results In 5 g/L ALA-PDT group, the growth of P.acnes at each concentration was inhibited completely. In 2.5 g/L ALA-PDT group, the growth of P.acnes at each concentration was not restrained. In 3.6 g/L ALA-PDT group, except the bacterium group of 107 CFU/mL, the growth of P.acnes was suppressed thoroughly. Conclusion In the condition of the same bacterial concentration, the inhibitory effect of ALA is positively correlated with its concentration.
