CAJ | 학술논문

背景：既往研究发现，miR-486在胶质瘤干细胞(CD133+)中的表达水平显著低于其在胶质瘤非干细胞(CD133-)中的表达水平，但是miR-486对CD133+细胞的影响尚不明确。目的：探索miR-486对CD133+细胞的作用。方法：利用流式细胞分选将 U87胶质瘤细胞中分为 CD133+和 CD133-细胞。通过脂质体转染构建 miR-486过表达的胶质瘤干细胞。结果与结论：流式细胞分选和纯化获得高比率的CD133+胶质瘤干细胞。实时反转录PCR检测发现miR-486在CD133+胶质瘤干细胞的表达水平比CD133-胶质瘤细胞明显下降。脂质体转染成功构建miR-486过表达的胶质瘤干细胞，体外实验发现miR-486高表达抑制胶质瘤干细胞的增殖，将其阻滞于G1/S期，并促进凋亡。提示miR-486对胶质瘤干细胞具有抑制作用。
배경：기왕연구발현，miR-486재효질류간세포(CD133+)중적표체수평현저저우기재효질류비간세포(CD133-)중적표체수평，단시miR-486대CD133+세포적영향상불명학。목적：탐색miR-486대CD133+세포적작용。방법：이용류식세포분선장 U87효질류세포중분위 CD133+화 CD133-세포。통과지질체전염구건 miR-486과표체적효질류간세포。결과여결론：류식세포분선화순화획득고비솔적CD133+효질류간세포。실시반전록PCR검측발현miR-486재CD133+효질류간세포적표체수평비CD133-효질류세포명현하강。지질체전염성공구건miR-486과표체적효질류간세포，체외실험발현miR-486고표체억제효질류간세포적증식，장기조체우G1/S기，병촉진조망。제시miR-486대효질류간세포구유억제작용。
BACKGROUND:Previous studies have found that the expression level of miR-486 in glioma stem cels (CD133+) is significantly down-regulated compared with that in glioma non-stem cels (CD133-), but the effect of down-regulation of miR-486 on CD133+ cels remains unclear . OBJECTIVE: To explore the effect of miR-486 on CD133+ cels. METHODS:CD133+ glioma stem cels and CD133- glioma cels were separated from U87 cels by flow cytometer. miR-486 overexpression glioma stem cels were constructed by lipofection transfection. RESULTS AND CONCLUSION:After sorting and purification, the content of the CD133+ fraction was enriched up to 83.5%. The expression level of miR-468 in CD133+ glioma stem cels was obviously down-regulated compared with that in CD133- glioma cels. CD133+ glioma stem cels overexpressing miR-486 were fabricated successfuly. Results from in vitro experiments showed that miR-486 overexpression could dramaticaly decrease the proliferation of glioma stem cels, induce a cel cycle arrest in G1/S phase for CD133+ glioma stem cels and promote cel apoptosis. These findings suggest that miR-486 can be a suppressor of glioma stem cels, which offers a novel potential therapeutic target for glioma stem cels and human glioma.