CAJ | 학술논문

背景：大量研究证实，新生血管形成在肿瘤的生长、浸润以及转移过程中发挥重要作用。目的：探讨CD133+卵巢癌干细胞样细胞向血管内皮细胞分化的特点。方法：通过无血清培养方法从卵巢癌A2780细胞株中成功诱导出CD133+卵巢癌干细胞样细胞，在体外接种于铺或不铺Matrigel基质胶的96孔板内，观察不同时间点CD133+卵巢癌干细胞样细胞和人脐静脉内皮细胞形成管腔样结构能力。通过裸鼠皮下移植实验，免疫荧光法观察CD133+卵巢癌干细胞样细胞在卵巢癌血管新生中的作用。结果与结论：CD133+卵巢癌干细胞样细胞和人脐静脉内皮细胞(阳性对照)在未铺 Matrigel基质胶上并不能形成相应的管腔结构，且不表达内皮细胞标志物 CD31，在 Matrigel 基质胶上能够形成相对稳定的管腔结构， CD31表达明显。CD133+卵巢癌干细胞样细胞接种裸鼠皮下成瘤后，可观察到肿瘤组织中有人源性CD31的表达。结果表明CD133+卵巢癌干细胞样细胞能够分化为血管内皮细胞，参与肿瘤血管重建。
배경：대량연구증실，신생혈관형성재종류적생장、침윤이급전이과정중발휘중요작용。목적：탐토CD133+란소암간세포양세포향혈관내피세포분화적특점。방법：통과무혈청배양방법종란소암A2780세포주중성공유도출CD133+란소암간세포양세포，재체외접충우포혹불포Matrigel기질효적96공판내，관찰불동시간점CD133+란소암간세포양세포화인제정맥내피세포형성관강양결구능력。통과라서피하이식실험，면역형광법관찰CD133+란소암간세포양세포재란소암혈관신생중적작용。결과여결론：CD133+란소암간세포양세포화인제정맥내피세포(양성대조)재미포 Matrigel기질효상병불능형성상응적관강결구，차불표체내피세포표지물 CD31，재 Matrigel 기질효상능구형성상대은정적관강결구， CD31표체명현。CD133+란소암간세포양세포접충라서피하성류후，가관찰도종류조직중유인원성CD31적표체。결과표명CD133+란소암간세포양세포능구분화위혈관내피세포，삼여종류혈관중건。
BACKGROUND:Numerous studies have confirmed that neovascularization plays an important role in the growth, invasion and metastasis of tumors. OBJECTIVE:To investigate the features of CD133+ ovarian cancer stem-like cels differentiating into vascular endothelial cels. METHODS:CD133+ ovarian cancer stem-like cels were successfuly harvested from A2780 ovarian cancer cel lines using serum-free culture method, and incubatedin vitro onto 96-wel plates with or without Matrigel. Then, we observed the capacity of CD133+ ovarian cancer stem-like cels and human umbilical vein endothelial cels to form tube-like structures at different time points. Through xenograft experiments, the role of CD133+ ovarian cancer stem-like cels in the angiogenesis of ovarian cancer was observed using immunofluorescence staining. RESULTS AND CONCLUSION:CD133+ovarian cancer stem-like cels and human umbilical vein endothelial cels cultured with no Matrigel had no corresponding lumen formation, and could not express CD31. But those cultured with Matrigel had lumen formation and expressed CD31 significantly. After tumor formation, human-derived CD31 expression was observed in the tumors. These findings indicate that CD133+ ovarian cancer stem-like cels can differentiate into vascular endothelial cels, and be involved in tumor revascularization.