CAJ | 학술논문

背景：异种韧带易于获得，具有韧带支架结构，有利于组织长入和爬行替代，经处理可完全消除抗原性，不引起免疫排斥反应，具有良好生长支架功能。目的：探索生物型异种韧带替代同种异体韧带移植重建山羊前交叉韧带损伤的可行性。方法：将24只健康山羊随机等分成A，B，C三组，取羊的左膝关节，制作成前交叉韧带断裂缺失模型，建立胫骨、股骨端骨隧道后，A，B，C组分别植入复合骨髓间充质干细胞的生物型异种韧带、生物型异种韧带和同种异体韧带。结果与结论：复合骨髓间充质干细胞的生物型异种韧带植入山羊体内后，无明显排斥反应，具有良好的组织相容性，植入韧带作为功能性支架重建前交叉韧带，在动物膝关节内环境的诱导下，使自体新生组织长入并替代支架，形成自体新生韧带，骨腱部愈合良好，但其与同种异体韧带重建前交叉韧带在组织学、免疫反应、生物力学方面差异无显著性意义，而植入复合骨髓间充质干细胞的生物型异种韧带山羊宿主自体组织可以长入并建立微循环。提示复合骨髓间充质干细胞的生物型异种韧带可加速微循环建立，促进韧带生长，尤其对韧带再血管化作用显著，但对韧带生物力学无明显影响。
배경：이충인대역우획득，구유인대지가결구，유리우조직장입화파행체대，경처리가완전소제항원성，불인기면역배척반응，구유량호생장지가공능。목적：탐색생물형이충인대체대동충이체인대이식중건산양전교차인대손상적가행성。방법：장24지건강산양수궤등분성A，B，C삼조，취양적좌슬관절，제작성전교차인대단렬결실모형，건립경골、고골단골수도후，A，B，C조분별식입복합골수간충질간세포적생물형이충인대、생물형이충인대화동충이체인대。결과여결론：복합골수간충질간세포적생물형이충인대식입산양체내후，무명현배척반응，구유량호적조직상용성，식입인대작위공능성지가중건전교차인대，재동물슬관절내배경적유도하，사자체신생조직장입병체대지가，형성자체신생인대，골건부유합량호，단기여동충이체인대중건전교차인대재조직학、면역반응、생물역학방면차이무현저성의의，이식입복합골수간충질간세포적생물형이충인대산양숙주자체조직가이장입병건립미순배。제시복합골수간충질간세포적생물형이충인대가가속미순배건립，촉진인대생장，우기대인대재혈관화작용현저，단대인대생물역학무명현영향。
BACKGROUND:Xenogeneic ligament is readily available, which has ligament scaffold structure and is conducive to tissue ingrowth and creeping substitution. After processing, the xenogeneic ligament, with the presence of good growth scaffold function, can be completely eliminated antigenicity that can cause immune rejection. OBJECTIVE:To study the feasibility of biological xenogeneic ligament graft instead of alogeneic ligament graft for reconstruction of goat anterior cruciate ligament. METHODS: Twenty-four healthy adult goats were randomly divided into A, B, C groups, and then, the left knee joints of goats were removed to establish animal models of anterior cruciate ligament injury in the three groups. After the establishment of the tibia and femoral bone tunnel, groups A, B, C were respectively transplanted with biological xenogeneic ligament graft combined with bone marrow mesenchymal stem cels, biological xenogeneic ligament graft alone, and alogenic ligament. RESULTS AND CONCLUSION: After implantation, no rejection but good biocompatibility was found in the group A, in which, the transplanted ligament as a functional scaffold for anterior cruciate ligament reconstruction, and new tissues in-grew and replaced the scaffold under intraarticular environment to form the new ligament with good bone tendon healing. However, there were no differences in histology, immune response, biomechanical findings between groups A and C. Additionaly, in the group A, the host tissues were found to grow into the scaffold and establish a micro-circulation. These findings indicate that xenogeneic ligament combined with bone marrow mesenchymal stem cels can accelerate the establishment of micro-circulation and promote the growth of ligaments, especialy improve the ligament revascularization significantly, but has no influence on the biological characteristics of the ligament.