中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2015年
41期
6616-6622
,共7页
干细胞%脐带脐血干细胞%脐血%间充质干细胞%心肌梗死%移植%心功能
榦細胞%臍帶臍血榦細胞%臍血%間充質榦細胞%心肌梗死%移植%心功能
간세포%제대제혈간세포%제혈%간충질간세포%심기경사%이식%심공능
Myocardium%Blood Vessels%Cord Blood Stem Cel Transplantation%Coronary Occlusion%Tissue Engineering
背景：胎儿脐血间充质干细胞对病变心肌有修复和再生能力，胎儿脐血间充质干细胞移植是治疗心肌梗死的一种新途径。目的：探讨胎儿脐血间充质干细胞移植治疗大鼠心肌梗死的效果。方法：选取32只大鼠结扎左冠状动脉前降支制作心肌梗死动物模型，随机等分为移植组和梗死组，从胎儿脐血中分离培养脐血间充质干细胞，制备脐血间充质干细胞悬液，对移植组大鼠进行脐血间充质干细胞移植。结果与结论：胎儿脐血间充质干细胞在体外可以被成功分离培养；与梗死组相比，移植组大鼠心肌梗死边缘区的微血管密度、左室收缩末压、左室内压最大上升和下降速率显著增加(P <0.05)，左室舒张末压显著下降(P <0.05)，心电图情况稍有好转。表明胎儿脐血间充质干细胞治疗心肌梗死大鼠可以促进心肌血管再生，改善心脏功能。
揹景：胎兒臍血間充質榦細胞對病變心肌有脩複和再生能力，胎兒臍血間充質榦細胞移植是治療心肌梗死的一種新途徑。目的：探討胎兒臍血間充質榦細胞移植治療大鼠心肌梗死的效果。方法：選取32隻大鼠結扎左冠狀動脈前降支製作心肌梗死動物模型，隨機等分為移植組和梗死組，從胎兒臍血中分離培養臍血間充質榦細胞，製備臍血間充質榦細胞懸液，對移植組大鼠進行臍血間充質榦細胞移植。結果與結論：胎兒臍血間充質榦細胞在體外可以被成功分離培養；與梗死組相比，移植組大鼠心肌梗死邊緣區的微血管密度、左室收縮末壓、左室內壓最大上升和下降速率顯著增加(P <0.05)，左室舒張末壓顯著下降(P <0.05)，心電圖情況稍有好轉。錶明胎兒臍血間充質榦細胞治療心肌梗死大鼠可以促進心肌血管再生，改善心髒功能。
배경：태인제혈간충질간세포대병변심기유수복화재생능력，태인제혈간충질간세포이식시치료심기경사적일충신도경。목적：탐토태인제혈간충질간세포이식치료대서심기경사적효과。방법：선취32지대서결찰좌관상동맥전강지제작심기경사동물모형，수궤등분위이식조화경사조，종태인제혈중분리배양제혈간충질간세포，제비제혈간충질간세포현액，대이식조대서진행제혈간충질간세포이식。결과여결론：태인제혈간충질간세포재체외가이피성공분리배양；여경사조상비，이식조대서심기경사변연구적미혈관밀도、좌실수축말압、좌실내압최대상승화하강속솔현저증가(P <0.05)，좌실서장말압현저하강(P <0.05)，심전도정황초유호전。표명태인제혈간충질간세포치료심기경사대서가이촉진심기혈관재생，개선심장공능。
BACKGROUND:Human umbilical cord blood-derived mesenchymal stem cels are able to repair and regenerate the injured myocardium, which is a new therapy for myocardial infarctionvia transplantation. OBJECTIVE:To explore the therapeutic efficacy of intracoronary injection of human umbilical cord blood-derived mesenchymal stem cels on acute myocardial infarction in rats. METHODS:Thirty-two rats were selected to make animal models of ligation of the left anterior descending coronary, and then model rats were randomized equaly to transplantation group and model group. Human umbilical cord blood-derived mesenchymal stem cels were isolated and prepared into cel suspension. Rats in the transplantation group were subjected to transplantation of human umbilical cord blood-derived mesenchymal stem cels. RESULTS AND CONCLUSION: Human umbilical cord blood-derived mesenchymal stem cels were successfuly isolated and cultured in vitro. Compared with the model group, the microvessel density, left ventricular end-systolic pressure and ±dp/dtmax were significantly increased in the transplantation group (P < 0.05), while the left ventricular end-diastolic pressure was decreased dramaticaly (P < 0.05). Electrocardiography findings showed that the heart function of rats in the transplantation group was improved slightly. These findings indicate that human umbilical cord blood-derived mesenchymal stem cels can promote myocardial angiogenesis and improve heart function of rats with myocardial infarction.
