CAJ | 학술논문

背景：骨质疏松是一种好发于绝经后妇女的慢性骨代谢疾病，随着世界人口老龄化的增加，如何预防和治疗绝经后骨质疏松是目前困扰医疗界的一大难题。目的：探讨氯化锂对去卵巢骨质疏松大鼠骨微结构和骨髓基质细胞分化的影响。方法：将30只3月龄雌性健康未孕SD大鼠去卵巢，因2只大鼠由于感染死亡，将剩下28只大鼠随机分为去卵巢体内组(9只)、去卵巢体外组(10只)和氯化锂组(9只)。手术后第11周，氯化锂组按照体质量每周腹腔注射3次氯化锂，剂量为15 mg/kg，干预8周后，用micro-CT检测9只去卵巢体内组和9只氯化锂组大鼠左侧股骨的骨微结构。10只去卵巢体外组大鼠的双侧股骨和胫骨用于骨髓基质细胞的培养，接种24 h后加入氯化锂，分为0 mmol/L(对照组)、1 mmol/L组和5 mmol/L组，培养至第6天和第8天更换培养基并加入相应浓度氯化锂，培养第10天处理细胞，用Western blot检测其SP7、Runx2和PPARγ2蛋白的表达水平。结果与结论：①体内结果表明，氯化锂组体积骨密度、骨体积分数和骨小梁数目显著高于去卵巢体内组，骨小梁间隙显著低于去卵巢体内组，而骨小梁厚度和结构模型指数无显著变化。②体外结果表明，1 mmol/L和5 mmol/L氯化锂组骨髓基质细胞Sp7和Runx2蛋白表达水平显著高于对照组，而PPARγ2蛋白表达水平显著低于对照组。③以上实验结果表明，氯化锂可能是通过促进去卵巢骨质疏松大鼠骨髓基质细胞向成骨分化而改善去卵巢骨质疏松大鼠的骨微结构。
배경：골질소송시일충호발우절경후부녀적만성골대사질병，수착세계인구노령화적증가，여하예방화치료절경후골질소송시목전곤우의료계적일대난제。목적：탐토록화리대거란소골질소송대서골미결구화골수기질세포분화적영향。방법：장30지3월령자성건강미잉SD대서거란소，인2지대서유우감염사망，장잉하28지대서수궤분위거란소체내조(9지)、거란소체외조(10지)화록화리조(9지)。수술후제11주，록화리조안조체질량매주복강주사3차록화리，제량위15 mg/kg，간예8주후，용micro-CT검측9지거란소체내조화9지록화리조대서좌측고골적골미결구。10지거란소체외조대서적쌍측고골화경골용우골수기질세포적배양，접충24 h후가입록화리，분위0 mmol/L(대조조)、1 mmol/L조화5 mmol/L조，배양지제6천화제8천경환배양기병가입상응농도록화리，배양제10천처리세포，용Western blot검측기SP7、Runx2화PPARγ2단백적표체수평。결과여결론：①체내결과표명，록화리조체적골밀도、골체적분수화골소량수목현저고우거란소체내조，골소량간극현저저우거란소체내조，이골소량후도화결구모형지수무현저변화。②체외결과표명，1 mmol/L화5 mmol/L록화리조골수기질세포Sp7화Runx2단백표체수평현저고우대조조，이PPARγ2단백표체수평현저저우대조조。③이상실험결과표명，록화리가능시통과촉진거란소골질소송대서골수기질세포향성골분화이개선거란소골질소송대서적골미결구。
BACKGROUND:Osteoporosis is a bone metabolic disease that affects women more than men. Prevention and treatment of osteoporosis is becoming a serious medical problem because of the aging of the population. OBJECTIVE:To explore the effects of lithium chloride treatment on bone microarchitecture and bone marrow stromal cel differentiation of ovariectomized osteoporosis rats. METHODS:After ovariectomy, 28 of 30 healthy female Sprague-Dawley rats, 3 months old, were randomly divided into the folowing three groups: ovariectomizedin vivo group (9 rats), ovariectomizedin vitro group (10 rats), and lithium chloride group (9 rats). At the 11th week postoperatively, rats in the lithium chloride were intragastricaly injected with lithium chloride at a dose of 15 mg/kg, three times per week. After 8 weeks of treatment, the bone microarchitectures of the rat left femur in the ovariectomizedin vivo group and lithium chloride group were detected by micro-CT. The bone marrow mesenchymal stem cels were freshly isolated from the bone marrow of the bilateral femurs and tibia of rats in the ovariectomizedin vitro group. After 24 hours of inoculation, the cels were cultured in lithium chloride and divided into 0 mmol/L (control), 1 mmol/L and 5 mmol/L groups. At 6 and 8 days of culture, the medium was changed and lithium chloride with the corresponding concentrations was added. At 10 days of culture, western blot assay was adopted to detect protein expression of Runx-2, SP7 and PPARγ2. RESULTS AND CONCLUSION:(1) Compared with the ovariectomizedin vivo group, the volume density of trabecular bone, number of trabecular bone, and bone volume fraction in the lithium chloride group were significantly increased and the separation of trabecular bone was significantly decreased. However, no differences were seen in the thickness of trabecular bone and structure model index. (2) Lithium chloride at 1 and 5 mmol/L could increase the protein expression of Sp7 and Runx-2 in bone marrow stromal cels, but decrease the protein expression ofPPARγ2. These results indicate that lithium chloride may improve the microarchitecture of the trabeculr bone in ovariectomized osteoporosis rats through stimulating the osteogenic differentiation of bone marrow stromal cels.