CAJ | 학술논문

背景：近年来，干细胞治疗股骨头早期骨坏死已经成为一种可选的方法，但干细胞质量影响治疗效果。目的：评价激素性股骨头坏死模型大鼠骨髓间充质干细胞的增殖能力以及定向诱导分化能力。方法：20只SD大鼠随机分为对照组和观察组，每组10只，观察组构建激素性股骨头坏死模型，分离培养两组大鼠骨髓间充质干细胞。采用CCK-8法检测第3代骨髓间充质干细胞增殖情况。选择第3代骨髓间充质干细胞进行成骨、成脂定向诱导分化。成骨诱导7，14 d行碱性磷酸酶染色和茜素红染色，成脂诱导21 d行油红O染色。结果与结论：培养第1，3，5天观察组细胞吸光度值均低于对照组，差异均无显著性意义(P >0.05)；但第7天时观察组吸光度值显著低于对照组，差异有显著性意义(P<0.05)。观察组碱性磷酸酶活性显著低于对照组(P <0.05)。与对照组比较，观察组的钙结节和脂滴数量较少。以上结果表明激素性股骨头坏死模型大鼠骨髓间充质干细胞的增殖能力和成骨成脂诱导分化能力均减弱。
배경：근년래，간세포치료고골두조기골배사이경성위일충가선적방법，단간세포질량영향치료효과。목적：평개격소성고골두배사모형대서골수간충질간세포적증식능력이급정향유도분화능력。방법：20지SD대서수궤분위대조조화관찰조，매조10지，관찰조구건격소성고골두배사모형，분리배양량조대서골수간충질간세포。채용CCK-8법검측제3대골수간충질간세포증식정황。선택제3대골수간충질간세포진행성골、성지정향유도분화。성골유도7，14 d행감성린산매염색화천소홍염색，성지유도21 d행유홍O염색。결과여결론：배양제1，3，5천관찰조세포흡광도치균저우대조조，차이균무현저성의의(P >0.05)；단제7천시관찰조흡광도치현저저우대조조，차이유현저성의의(P<0.05)。관찰조감성린산매활성현저저우대조조(P <0.05)。여대조조비교，관찰조적개결절화지적수량교소。이상결과표명격소성고골두배사모형대서골수간충질간세포적증식능력화성골성지유도분화능력균감약。
BACKGROUND:In recent years, stem cel therapy for early osteonecrosis of the femoral head has become an alternative method, but the quality of stem cels is a key to the therapeutic outcomes. OBJECTIVE:To evaluate the proliferative ability and directional differentiation ability of autologous bone marrow mesenchymal stem cels in a rat model of steroid-induced femoral head necrosis. METHODS:Twenty Sprague-Dawley rats were randomly divided into control and observation groups with ten in each group. An animal model of steroid-induced femoral head necrosis was built in the observation group, and then bone marrow mesenchymal stem cels from rats in both two groups were isolated and cultured. Cel counting kit-8 was used to detect proliferation of passage 3 cels. Bone marrow mesenchymal stem cels at passage 3 were selected in the two groups for osteogenic and adipogenic induction. Alkaline phosphatase staining and alizarin red staining were adopted at 7 and 14 days of osteogenic induction, and oil red O staining as performed at 21 days of adipogenic induction. RESULTS AND CONCLUSION:The absorbance values of bone marrow mesenchymal stem cels were lower in the observation group than the control group at 1, 3, 5 days of culture, but there was no significant difference between two groups (P > 0.05). Until the 7th day of culture, the absorbance value and alkaline phosphatase activity in the observation group were significantly lower than that in the control group (P < 0.05). Additionaly, there were fewer calcium nodules and lipid droplets in the observation group compared with the control group. These findings suggest that the proliferative ability and directional differentiation ability of autologous bone marrow mesenchymal stem cels from a rat model of steroid-induced femoral head necrosis are both decreased.