CAJ | 학술논문

背景：自2003年，美国 FDA率先批准自体骨髓干细胞移植治疗心肌梗死性疾病以来，已有大量临床及基础研究报道，但报道结果却不尽相同，其中很重要的一点就是干细胞无法有效到达心肌损伤部位(即干细胞归巢)。目的：探讨小鼠骨髓间充质干细胞各亚群在心肌修复中的归巢能力。方法：以小鼠心肌干细胞表面分化抗原筛查小鼠骨髓间充质干细胞，再以CD45、CD31为标准分选得到小鼠骨髓间充质干细胞4个亚群。采用 Transwel 小室检测各亚群组细胞的归巢能力。将各亚群细胞注入心肌梗死48 h小鼠体内，注射后48，96 h及7 d处死小鼠取其心脏完成小动物活体成像并检测其荧光强度。结果与结论：小鼠骨髓间充质干细胞 SCA-1+/CD45+/CD31+亚群归巢能力优于其他亚群。小动物活体成像提示干细胞注射48 h，96 h及7 d时SCA-1+/CD45+/CD31+亚群平均荧光强度优于其他亚群，SCA-1+/CD45+/ CD31+亚群迁移率最高，说明SCA-1+/CD45-/CD31-群体有向受损心肌归巢的趋势。
배경：자2003년，미국 FDA솔선비준자체골수간세포이식치료심기경사성질병이래，이유대량림상급기출연구보도，단보도결과각불진상동，기중흔중요적일점취시간세포무법유효도체심기손상부위(즉간세포귀소)。목적：탐토소서골수간충질간세포각아군재심기수복중적귀소능력。방법：이소서심기간세포표면분화항원사사소서골수간충질간세포，재이CD45、CD31위표준분선득도소서골수간충질간세포4개아군。채용 Transwel 소실검측각아군조세포적귀소능력。장각아군세포주입심기경사48 h소서체내，주사후48，96 h급7 d처사소서취기심장완성소동물활체성상병검측기형광강도。결과여결론：소서골수간충질간세포 SCA-1+/CD45+/CD31+아군귀소능력우우기타아군。소동물활체성상제시간세포주사48 h，96 h급7 d시SCA-1+/CD45+/CD31+아군평균형광강도우우기타아군，SCA-1+/CD45+/ CD31+아군천이솔최고，설명SCA-1+/CD45-/CD31-군체유향수손심기귀소적추세。
BACKGROUND:Since the FDA was the first to approve autologous bone marrow stem cel transplantation for treatment of myocardial infarction in 2003, there has a large number of clinical and basic research reports. However, their conclusions are different and stem cel homing is a key point. OBJECTIVE:To explore the homing abilities of different subgroups of mouse bone marrow mesenchymal stem cels in myocardial regeneration. METHODS:After mouse bone marrow mesenchymal stem cels were detected using a mouse cardiac stem cel surface differentiation antigen, four cel subgroups were separated on the basis of CD45 and CD31. The homing abilities of the four subgroups were assayed in a Transwel chamberin vitro. The different cel subgroups were injected into the model mice suffering from myocardial infarction for 48 hours. The mice were sacrificed at 48 hours, 96 hours, and 7 days after injection; the hearts were taken and analyzed through whole-body imaging and fluorescence intensity detection. RESULTS AND CONCLUSION:The SCA-1+/CD45+/CD31+ subgroup exhibited the strongest homing ability. The whole-body imaging indicated that the fluorescence intensity of SCA-1+/CD45+/CD31+ subgroup was higher than that of the other subgroups at 48 hours, 96 hours and 7 days after stem cel injection. The migration rate of SCA-1+/CD45+/CD31+ subgroup was also the highest. These findings indicate that the homing ability of the SCA-1+/CD45+/CD31+ subgroup of mouse bone marrow mesenchymal stem cels exhibit a homing trend to the damaged myocardial tissue.