CAJ | 학술논문

目的探究富含n-3多不饱和脂肪酸( polyunsaturated fatty acid ,PUFA)的苏子油高脂饮食对肥胖大鼠肝脏极低密度脂蛋白( very low density lipoproteins ,VLDL)合成关键基因表达的影响. 方法造模期:雄性SD大鼠随机分为2组,对照组(normal control group, NC)给予普通日粮,高脂组(high fat group, HF)给予高脂纯合日粮诱发肥胖大鼠模型;干预期:将肥胖大鼠随机分为4组:持续高脂组( consistent high fat group , CHF)和三个苏子油( perilla oil, PO)替代组,根据PO替代CHF中猪油比例的不同将替代组分为20%PO、50%PO、100%PO,4周后测定大鼠血清甘油三酯( tryglyceride, TG)水平;western blotting方法检测肝脏VLDL合成关键蛋白微粒体甘油三脂转运蛋白(microsomal triglyceride transfer protein,MTP)和载脂蛋白B(apolipoprotein B,APOB)蛋白表达;real-time PCR方法检测肝脏Mtp、Apob mRNA表达. 结果肥胖大鼠血清TG水平显著高于NC组,并且有严重的脂肪沉积,肝脏MTP和APOB的mRNA、蛋白表达均有不同程度的降低;干预4周后,与CHF组相比,各PO替代组大鼠血清TG水平明显降低,病理切片结果显示肝脏脂肪沉积有明显改善,并且肝脏MTP和APOB的mRNA、蛋白表达均有不同程度的升高. 结论不同比例PO替代均能促进肥胖大鼠肝脏VLDL合成与分泌,改善肝脏脂肪沉积,并且PO促进Mtp mRNA、蛋白表达具有剂量依赖性.
목적 탐구부함n-3다불포화지방산( polyunsaturated fatty acid ,PUFA)적소자유고지음식대비반대서간장겁저밀도지단백( very low density lipoproteins ,VLDL)합성관건기인표체적영향. 방법 조모기:웅성SD대서수궤분위2조,대조조(normal control group, NC)급여보통일량,고지조(high fat group, HF)급여고지순합일량유발비반대서모형;간예기:장비반대서수궤분위4조:지속고지조( consistent high fat group , CHF)화삼개소자유( perilla oil, PO)체대조,근거PO체대CHF중저유비례적불동장체대조분위20%PO、50%PO、100%PO,4주후측정대서혈청감유삼지( tryglyceride, TG)수평;western blotting방법검측간장VLDL합성관건단백미립체감유삼지전운단백(microsomal triglyceride transfer protein,MTP)화재지단백B(apolipoprotein B,APOB)단백표체;real-time PCR방법검측간장Mtp、Apob mRNA표체. 결과 비반대서혈청TG수평현저고우NC조,병차유엄중적지방침적,간장MTP화APOB적mRNA、단백표체균유불동정도적강저;간예4주후,여CHF조상비,각PO체대조대서혈청TG수평명현강저,병리절편결과현시간장지방침적유명현개선,병차간장MTP화APOB적mRNA、단백표체균유불동정도적승고. 결론 불동비례PO체대균능촉진비반대서간장VLDL합성여분비,개선간장지방침적,병차PO촉진Mtp mRNA、단백표체구유제량의뢰성.
Objective This study aims to investigate the effects of high-fat diet rich in perilla oil on the expression of key genes that regulate hepatic VLDL synthesis in obese rats .Methods Sixty healthy male 5-week old SD rats were randomly divided into 2 groups.The rats in the normal control group (NC, n=12) were given normal diet, and the rats in the high fat group ( HF, n=48) were given a pure high fat diet in order to induce rat models of obesity .In the intervention period, the obesity model rats were randomly divided into 4 subgroups including consistent high fat group (CHF) and three intervention groups depending on perilla oil substitution rate of lard in CHF:20%PO, 50%PO and 100%PO.The serum triglyceride (TG) of the rats was measured after 4 weeks.Real-time PCR was applied to measure microsomal triglyceride transfer protein ( Mtp) and apolipoprotein B ( Apob) mRNA, and western blot assay was used for detecting the expression of MTP and APOB in the liver .Results Compared with the NC group , the CHF rats exhibited significantly high fat deposi-tion.The serum TG was markedly higher and the MTP and APOB were decreased at gene and protein levels in the CHF group compared with the NC group .After the intervention , PO remarkably reduced the level of serum TG and decreased he-patic fat deposition as it showed by pathological examination .At the gene and protein levels , MTP and APOB were upregu-lated by PO to different degrees .Conclusions All the three PO intervention can promote VLDL synthesis and secretion , and decrease the hepatic fat deposition in the obese rats .Furthermore , PO upregulates the expression of MTP at gene and protein levels in a dose-dependent manner .