中国实验动物学报
中國實驗動物學報
중국실험동물학보
Acta Laboratorium Animalis Scientia Sinica
2015年
5期
513-516
,共4页
LPS%MPTP%帕金森病%小鼠模型%酪氨酸羟化酶
LPS%MPTP%帕金森病%小鼠模型%酪氨痠羥化酶
LPS%MPTP%파금삼병%소서모형%락안산간화매
Lipopolysaccharide%LPS%MPTP%Parkinson's disease(PD)%Rat model%Tyrosine hydroxylase
目的 建立脂多糖( lipopolysaccharide , LPS)联合1-甲基-4-苯基-1,2,3,6-四氢吡啶(1-methyl-4-phe-nyl-1,2,3,6-tetrahydropyridine , MPTP)诱导的慢性小鼠帕金森病模型,探讨其行为学和黒质多巴胺能神经元细胞的变化情况. 方法 将20只C57BL/6小鼠随机分为模型组和对照组,模型组每日腹腔注射LPS (0.25 mg/kg) 一次,连续3 d,最后一次注射LPS 4 h后,每日腹腔注射MPTP (25 mg/kg)一次,连续2 d,对照组腹腔注射相同量的生理盐水. 8周后用步态分析和转棒实验评价小鼠的行为学能力,免疫组化法观察黑质酪氨酸羟化酶 ( TH) 阳性细胞变化情况. 结果 模型组小鼠行为学变化较对照组差异有显著性( P<0.05 ) ,模型组小鼠中脑黒质区显示出严重的神经元细胞损伤. 结论 LPS联合MPTP腹腔注射可成功诱导出慢性小鼠帕金森病模型,提示该模型可用于帕金森病的发病机制及药物治疗效果等相关研究.
目的 建立脂多糖( lipopolysaccharide , LPS)聯閤1-甲基-4-苯基-1,2,3,6-四氫吡啶(1-methyl-4-phe-nyl-1,2,3,6-tetrahydropyridine , MPTP)誘導的慢性小鼠帕金森病模型,探討其行為學和黒質多巴胺能神經元細胞的變化情況. 方法 將20隻C57BL/6小鼠隨機分為模型組和對照組,模型組每日腹腔註射LPS (0.25 mg/kg) 一次,連續3 d,最後一次註射LPS 4 h後,每日腹腔註射MPTP (25 mg/kg)一次,連續2 d,對照組腹腔註射相同量的生理鹽水. 8週後用步態分析和轉棒實驗評價小鼠的行為學能力,免疫組化法觀察黑質酪氨痠羥化酶 ( TH) 暘性細胞變化情況. 結果 模型組小鼠行為學變化較對照組差異有顯著性( P<0.05 ) ,模型組小鼠中腦黒質區顯示齣嚴重的神經元細胞損傷. 結論 LPS聯閤MPTP腹腔註射可成功誘導齣慢性小鼠帕金森病模型,提示該模型可用于帕金森病的髮病機製及藥物治療效果等相關研究.
목적 건립지다당( lipopolysaccharide , LPS)연합1-갑기-4-분기-1,2,3,6-사경필정(1-methyl-4-phe-nyl-1,2,3,6-tetrahydropyridine , MPTP)유도적만성소서파금삼병모형,탐토기행위학화흑질다파알능신경원세포적변화정황. 방법 장20지C57BL/6소서수궤분위모형조화대조조,모형조매일복강주사LPS (0.25 mg/kg) 일차,련속3 d,최후일차주사LPS 4 h후,매일복강주사MPTP (25 mg/kg)일차,련속2 d,대조조복강주사상동량적생리염수. 8주후용보태분석화전봉실험평개소서적행위학능력,면역조화법관찰흑질락안산간화매 ( TH) 양성세포변화정황. 결과 모형조소서행위학변화교대조조차이유현저성( P<0.05 ) ,모형조소서중뇌흑질구현시출엄중적신경원세포손상. 결론 LPS연합MPTP복강주사가성공유도출만성소서파금삼병모형,제시해모형가용우파금삼병적발병궤제급약물치료효과등상관연구.
Objective The aim of this study was to establish a mouse model of chronic Parkinson ' s disease in-duced by systemic administration of lipopolysaccharide plus 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP), and to study the changes of behavioral manifestation , numbers of dopaminergic neurons in the substantia nigra pars compacta . Methods Twenty C57BL mice were randomly divided into 2 groups:the saline control group and model group .The mice in the model group received three intraperitoneal (i.p.) injections of LPS (0.25 mg/kg), once daily for three consecutive days.Four hours following the final LPS injection , the mice received one subcutaneous injection of low-dose MPTP (25 mg/kg).The mice of control group were injected with the same volume of saline .Eight weeks later, the motor ability of the mice was evaluated by footprint test and rotarod test .The tyrosine hydroxylase ( TH)-positive cells were observed by immu-nohistochemical analysis .Results Compared with the control group , the scores of behavioral test were significantly lower , numbers of TH immunoreactive cells were significantly less in the Parkinson ' s model group ( P<0.05 ) .Conclusions Behavioral manifestation ,number of dopaminergic neurons in the substantia nigra are significantly changed in the mouse models of Parkinson ' s disease produced by repeated injection of LPS plus MPTP , suggesting that this chronic animal model can be used in the experimental study for pathogenesis and therapy of Parkinson ' s disease .
