CAJ | 학술논문

目的正向遗传筛选斑马鱼肝脏、肠和胆囊发育缺陷突变体. 方法 ENU诱变野生型斑马鱼并开展经典的F2代筛选,以lfabp为探针的全胚原位杂交、BES-H2 O2-Ac荧光染料分别检测斑马鱼早期胚胎肝脏、肠和胆囊的表型. 结果在128个突变基因组中筛选获得了源自14个F2家族的斑马鱼消化器官发育缺陷突变体品系23个,并按表型划分为6类. 结论斑马鱼肝脏、肠和胆囊的发育调控机制有相似性和差异性.
목적 정향유전사선반마어간장、장화담낭발육결함돌변체. 방법 ENU유변야생형반마어병개전경전적F2대사선,이lfabp위탐침적전배원위잡교、BES-H2 O2-Ac형광염료분별검측반마어조기배태간장、장화담낭적표형. 결과 재128개돌변기인조중사선획득료원자14개F2가족적반마어소화기관발육결함돌변체품계23개,병안표형화분위6류. 결론 반마어간장、장화담낭적발육조공궤제유상사성화차이성.
Objective To obtain zebrafish mutants with developmental defects in digestive organs from a forward genetic screening .Methods ENU mutagenesis and a classical F 2 genetic screening were performed .RNA whole mount in-situ hybridization using lfabpas probe and BES-H2 O2-Ac staining was applied to examine the liver , intestine and gall blad-der phenotype in zebrafish embryos .Results We harvested 23 mutant lines with developmental defects in digestive organs (originated from 14 F2 families) after screening 128 mutagenized genomes.These mutants were classified into six groups according to their phenotypes .Conclusions The liver, intestine and gall bladder share and differ in their developmental molecular mechanisms in zebrafish .