CAJ | 학술논문

目的：探讨微乳薄层色谱法同时分离鉴别心脑脉通胶囊中多种有效成分。方法以聚酰胺薄膜为固定相，微乳液为展开剂，对处方中蓝萼香茶菜、葛根、丹参、川芎四味药材进行定性鉴别，并考察了十二烷基硫酸钠（SDS ）、油相、水相等因素对分离效果的影响。结果以含水量75％的微乳液[SDS-正丁醇-正庚烷-水，g／g ]-甲酸-丙酮（5∶1∶1）为展开剂，可同时分离鉴别心脑脉通中的蓝萼香茶菜、葛根、丹参、川芎，斑点清晰，且阴性对照无干扰，分离效果理想。结论微乳薄层色谱法操作简便，结果准确，重现性好，可同时分离鉴别中成药中的多种有效成分。
목적：탐토미유박층색보법동시분리감별심뇌맥통효낭중다충유효성분。방법이취선알박막위고정상，미유액위전개제，대처방중람악향다채、갈근、단삼、천궁사미약재진행정성감별，병고찰료십이완기류산납（SDS ）、유상、수상등인소대분리효과적영향。결과이함수량75％적미유액[SDS-정정순-정경완-수，g／g ]-갑산-병동（5∶1∶1）위전개제，가동시분리감별심뇌맥통중적람악향다채、갈근、단삼、천궁，반점청석，차음성대조무간우，분리효과이상。결론미유박층색보법조작간편，결과준학，중현성호，가동시분리감별중성약중적다충유효성분。
Objective To investigate the application of microemulsion thin layer chromatography (TLC) for separation and identification of various active components in Xinnaomaitong capsule .Methods The all-grass of Bluecalyx japanese rabo-dosia,rootof Puerarialobata,rootandrhizomeof Salviamiltiorrhiza,rhizomeof LigusticumchuanxionginXinnaomaitong capsule were separated and identified simultaneously with the polyamide film as stationary phase and the microemulsion as mo-bile phases .Different factors ,such as the sodium dodecyl sulfate (SDS) ,oil phase and water phase which effect on the separa-tion of the components were evaluated .Results The all-grass of Bluecalyx japanese rabodosia ,root of Pueraria lobata ,root andrhizomeof Salviamiltiorrhiza,rhizomeof LigusticumchuanxionginXinnaomaitongcapsulewereseparatedandidentified simultaneously with mobile phase of microemulsion∶formic acid∶acetone (5∶1∶1) .The constituent of microemulsion was SDS∶butyl alcohol∶n-Heptane∶water = 6 .7∶15 .8∶2 .5∶75 .0 (g/g) .The spots were clear ,the negative control was no interference ,and it showed an ideal separation .Conclusion Microemulsion TLC is simple ,accurate ,and reproducible .It can identify a variety of active components in traditional Chinese medicine simultaneously .