CAJ | 학술논문

为了以较低的成本充分提取葡萄籽中的活性物质,利用有机溶剂法对葡萄籽中的活性物质原花青素、葡萄籽油和白藜芦醇进行联合提取工艺研究.结果表明：有机溶剂法提取3种活性物质的6种不同组合中,原花青素的得率差异不显著,得率为163.196~164.740 mg/g；葡萄籽油得率最高的为 F组合[葡萄籽油(以石油醚为提取剂,料液比为1∶10,55℃浸提2次,每次30 min.)→白藜芦醇(以乙酸乙酯为提取剂,料液比为1∶10,30℃浸提2次,每次3 h)→原花青素(以70%乙醇为提取剂,料液比为1∶10,50℃下浸提2次,每次1 h)],得率达231.047 mg/g；白藜芦醇得率最大的为 E组合(3种成分的提取参数与 F组合相同,葡萄籽油→原花青素→白藜芦醇),得率为39.13μg/g,但与F组合的得率38.74 mg/g差异不显著,且E组合中获得白藜芦醇浓缩液色素含量较高,后续白藜芦醇的脱色纯化较为困难.综合3种物质的得率及后续的纯化工艺,F组合为最佳提取组合,即先提取葡萄籽油,其次提取白藜芦醇,最后提取原花青素.
위료이교저적성본충분제취포도자중적활성물질,이용유궤용제법대포도자중적활성물질원화청소、포도자유화백려호순진행연합제취공예연구.결과표명：유궤용제법제취3충활성물질적6충불동조합중,원화청소적득솔차이불현저,득솔위163.196~164.740 mg/g；포도자유득솔최고적위 F조합[포도자유(이석유미위제취제,료액비위1∶10,55℃침제2차,매차30 min.)→백려호순(이을산을지위제취제,료액비위1∶10,30℃침제2차,매차3 h)→원화청소(이70%을순위제취제,료액비위1∶10,50℃하침제2차,매차1 h)],득솔체231.047 mg/g；백려호순득솔최대적위 E조합(3충성분적제취삼수여 F조합상동,포도자유→원화청소→백려호순),득솔위39.13μg/g,단여F조합적득솔38.74 mg/g차이불현저,차E조합중획득백려호순농축액색소함량교고,후속백려호순적탈색순화교위곤난.종합3충물질적득솔급후속적순화공예,F조합위최가제취조합,즉선제취포도자유,기차제취백려호순,최후제취원화청소.
In order to extract the active substances from grape seeds at a low cost,the integrated extrac-tion of active substances,proanthocyanidins,grape seed oil and resveratrol,in grape seeds by using organic solvent were studied in this experiment.The results showed that the extracting rates of proanthocyanidins were not significantly different among the six extracting combinations,which were 163.196~164.740 mg/g.The highest extracting rate (231.047 mg/g)of grape seed oil was obtained from combination F, which was performed as grape seed oil (extracted with petroleum ether for two times at 55 ℃ and 30 min of each time,the ratio of material to liquid was 1∶10)→resveratrol (extracted with ethyl acetate for two times at 30 ℃ and 3 h of each time,the ratio of material to liquid was 1∶10)→proanthocyanidins (extracted with 70% alcohol for two times at 50 ℃ and 1 h of each time,the ratio of material to liquid was 1∶10).The highest extracting rate (3 9 .1 3μg/g)of resveratrol was obtained from combination E,which showed same while the difference was not significant compared with combination F.In addition,the pigment content was much higher in resveratrol concentrates for combination F,which led to the difficulty on subsequent decol-orization and purification.Considering the extracting rates of the three active substances and the subse-quent purification process,combination F showed the best performance at the extracting order of grape seed oil→resveratrol→proanthocyanidins.