CAJ | 학술논문

采用石蜡切片法,对蛇足石杉的不同器官进行了解剖学特征研究;结合冰冻切片法,用碘化铋钾、氯化铁染色法分别对蛇足石杉的根、茎和叶中的生物碱和黄酮类化合物进行了组织化学定位.结果表明:生物碱主要存在于蛇足石杉根和茎的中柱木质部和皮层的薄壁细胞中,少数存在于叶肉细胞及其胞间隙中,其含量大小顺序为:茎≈根>叶;黄酮类化合物主要存在于蛇足石杉的小型叶肉细胞及其胞间隙中,部分存在于根和茎的中柱韧皮部,含量大小顺序为:叶>茎≈根;在不同的器官中,生物碱和黄酮类化合物的含量存在差异,其中茎和根的生物碱含量较高,而叶中黄酮类化合物含量较高.
채용석사절편법,대사족석삼적불동기관진행료해부학특정연구;결합빙동절편법,용전화필갑、록화철염색법분별대사족석삼적근、경화협중적생물감화황동류화합물진행료조직화학정위.결과표명:생물감주요존재우사족석삼근화경적중주목질부화피층적박벽세포중,소수존재우협육세포급기포간극중,기함량대소순서위:경≈근>협;황동류화합물주요존재우사족석삼적소형협육세포급기포간극중,부분존재우근화경적중주인피부,함량대소순서위:협>경≈근;재불동적기관중,생물감화황동류화합물적함량존재차이,기중경화근적생물감함량교고,이협중황동류화합물함량교고.
The paraffin sections were used to study the characteristics of morphological and anatomic structure ;an improved dragendorff reagents ( bismuth potassium iodide reagent ) was used to locate the alkaloid in root , stem and leaf of Huperzia serrata;the ferric chloride reagent was used to locate the flavonoid in root , stem and leaf of H.serrata.The results showed that the color reaction and special crystal deposition sites indicated that the alkaloid was located in the stele xylem and parenchyma of root and stem , and mesophyll and its intercellu-lar space of leaf.The alkaloid content in stem was almost equal to that in root , which was in general higher than that in leaf.The flavonoid was located in mesophyll and its intercellular space of leaf , the stele phloem of root and stem.The flavonoid content in stem was almost equal to that in root , which was in general lower than that in leaf.The alkaloid and flavonoid contents were obviously different among the different organs of the same species.The highest alkaloid content was in the stem and root , and the highest flavonoid content was found in the leaf based on these observation.The value of this study resided not only in indicating that different organs having different medicinal value , but also in providing theoretical basis of tissue culture and cultivation and the selection of medicinal parts for H.serrata.