CAJ | 학술논문

Wnt10b、β-catenin、FGF18基因在‘甘肃高山细毛羊’胎儿皮肤毛囊中的表达规律研究
Wnt10b、β-catenin、FGF18기인재‘감숙고산세모양’태인피부모낭중적표체규률연구
Expression patterns of Wnt10b,β-catenin and FGF18 genes in fetal hair follicles of Gansu alpine fine-wool sheep

万方数据

甘肃农业大学学报甘肅農業大學學報 감숙농업대학학보
Journal of Gansu Agricultural University
2015年 5期 6-14 ,共9页

赵帅%岳耀敬%郭婷婷%吴瑜瑜%刘建斌%韩吉龙%郭健%牛春娥%孙晓萍%冯瑞林%王天翔%李桂英%李范文%史兆国%杨博辉

조수%악요경%곽정정%오유유%류건빈%한길룡%곽건%우춘아%손효평%풍서림%왕천상%리계영%리범문%사조국%양박휘

绵羊%次级毛囊%Wnt10b%β-catenin%FGF18 綿羊%次級毛囊%Wnt10b%β-catenin%FGF18
면양%차급모낭%Wnt10b%β-catenin%FGF18
sheep%secondary follicle morphogenesis%Wnt10b%β-catenin%FGF18

以81~147 d胎龄的‘甘肃高山细毛羊’胚胎体侧部皮肤作为研究材料,通过冰冻切片、HE染色、荧光定量P C R、免疫组化技术研究Wnt10b、β-catenin、FGF18基因在次级毛囊形态发生过程中的表达规律.结果表明：从次级毛囊形态发生诱导期到器官形成阶段(胎龄87~108 d)Wnt10 b、FGF18基因表达量逐渐升高,在胎龄第108天Wnt10b、FGF18基因相对表达量分别达到(43.652±0.425)(13.67±0.207),在细胞分化期表达量逐渐下降；而β-catenin基因的表达量始终维持较低水平,相对表达量仅为(0.58±015)；Wnt10b 基因分别与β-catenin、FGF18基因表达水平呈显著正相关(r=0.85,P<0.01；r=0.58,P<0.05)；β-catenin基因与FGF18基因表达水平呈正相关(r=0.43,P>0.05).免疫组化结果显示在次级毛囊形态发生过程中Wnt10b、β-catenin 基因主要在表皮、基板、毛芽、毛钉和成熟毛囊的内根鞘、毛干部表达；而FGF18不仅在表皮、基板、毛芽、毛钉和成熟毛囊的内根鞘、毛干部表达,而且在真皮中表达.本研究初步表明,Wnt10b、β-catenin、FGF18基因通过Wnt/β-catenin 基因经典信号通路参与调控‘甘肃高山细毛羊’次级毛囊形态发生和再分化过程.
이81~147 d태령적‘감숙고산세모양’배태체측부피부작위연구재료,통과빙동절편、HE염색、형광정량P C R、면역조화기술연구Wnt10b、β-catenin、FGF18기인재차급모낭형태발생과정중적표체규률.결과표명：종차급모낭형태발생유도기도기관형성계단(태령87~108 d)Wnt10 b、FGF18기인표체량축점승고,재태령제108천Wnt10b、FGF18기인상대표체량분별체도(43.652±0.425)(13.67±0.207),재세포분화기표체량축점하강；이β-catenin기인적표체량시종유지교저수평,상대표체량부위(0.58±015)；Wnt10b 기인분별여β-catenin、FGF18기인표체수평정현저정상관(r=0.85,P<0.01；r=0.58,P<0.05)；β-catenin기인여FGF18기인표체수평정정상관(r=0.43,P>0.05).면역조화결과현시재차급모낭형태발생과정중Wnt10b、β-catenin 기인주요재표피、기판、모아、모정화성숙모낭적내근초、모간부표체；이FGF18불부재표피、기판、모아、모정화성숙모낭적내근초、모간부표체,이차재진피중표체.본연구초보표명,Wnt10b、β-catenin、FGF18기인통과Wnt/β-catenin 기인경전신호통로삼여조공‘감숙고산세모양’차급모낭형태발생화재분화과정.
The expression patterns of Wnt10b,β-catenin and FGF18 genes during the morphogenesis of secondary follicles (SF)in fetal hair follicles of Gansu alpine fine-wool sheep were analyzed in the present study.The midside skins of the embryos of Gansu alpine fine-wool sheep with a gestational age (GA)of 81~147 d were used as the study objects.The expression patterns of Wnt10b,β-catenin and FGF18 genes during the morphogenesis of SF were studied through frozen sections,hematoxylin and eosin (H.E)stai-ning,real-time quantitative polymerase chain reaction (PCR)and immunohistochemical techniques.The re-sults showed that the morphogenesis of the secondary-original follicles(SO)of the Gansu alpine fine-wool sheep underwent 3 periods and 8 steps before the follicles matured.The original SO could further differen-tiate into secondary-derived follicles (SD).The gene expression levels of Wnt10b,β-catenin and FGF18 gradually increased from the induction phase of the SF morphogenesis to the organogenesis period (GA 87-108 d).At GA 108 d,the relative expression levels of Wnt10b and FGF18 reached (43.652±0.425)and (13.67±0.207),respectively,and then gradually decreased during the cell differentiation period.However, the gene expression ofβ-catenin remained at a relatively low level,while its relative expression level was only (0.58±015).The correlations among Wnt10b,β-catenin and FGF18 expressions showed that FGF18 and Wnt10b,beta catenin expression quantity were positively correlated (r=0.58,P<0.05 and r=0.05,P>0.05).The immunohistochemical results showed that Wnt10b andβ-catenin were expressed primarily in the epidermis,the hair placode,the hair buds,the hair nails,the inner root sheaths and the hair shafts of the mature follicles.FGF18 was not only expressed in the epidermis,the hair placode,the hair buds,the hair nails,the inner root sheaths and the hair shafts of the mature follicles,but also expressed in the dermis.So, we proposed that Wnt10b,β-catenin and FGF18 genes may be involved in regulating of SF morphogenesis through the canonical Wnt/β-catenin signaling pathways.