CAJ | 학술논문

目的研究不同生物群解脲支原体对精液白细胞及精子运动参数的影响.方法使用培养法检测223例男性不育患者及146例可生育人群解脲支原体的感染率,RT-PCR法鉴定生物群,彩色精子自动分析系统分析精液白细胞及精子运动参数.结果不育组精子的曲线速度(VCL)、直线速度(VSL)、平均路径速度(VAP)、鞭打频率(BCF)与正常组之间差异具有统计学意义(P＜0.05);生物Ⅱ群解脲支原体(biovarⅡ)感染组白细胞异常增高率与未感染组之间差异具有统计学意义(P ＜0.001),与未感染组比较biovarⅡ感染组的精子运动参数[VCL(P＜0.001)、VSL(P=0.002)、VAP(P=0.024)、运动直线性(LIN)(P=0.009)、摆动性(WOB)(P=0.004)]差异均具有统计学意义.结论解脲支原体biovarⅡ感染与精子运动参数下降和白细胞异常增高密切相关.
목적 연구불동생물군해뇨지원체대정액백세포급정자운동삼수적영향.방법 사용배양법검측223례남성불육환자급146례가생육인군해뇨지원체적감염솔,RT-PCR법감정생물군,채색정자자동분석계통분석정액백세포급정자운동삼수.결과 불육조정자적곡선속도(VCL)、직선속도(VSL)、평균로경속도(VAP)、편타빈솔(BCF)여정상조지간차이구유통계학의의(P＜0.05);생물Ⅱ군해뇨지원체(biovarⅡ)감염조백세포이상증고솔여미감염조지간차이구유통계학의의(P ＜0.001),여미감염조비교biovarⅡ감염조적정자운동삼수[VCL(P＜0.001)、VSL(P=0.002)、VAP(P=0.024)、운동직선성(LIN)(P=0.009)、파동성(WOB)(P=0.004)]차이균구유통계학의의.결론 해뇨지원체biovarⅡ감염여정자운동삼수하강화백세포이상증고밀절상관.
Objective To investigate the effects of two biovars of ureaplasma urealyticum on leukocyte concentration and sperm motion parameters.Methods Sperm motion parameters were analyzed with computer-aided sperm analysis (CASA).Leukocytic number of semen was measured with benziding-perxidase staining.Culturing and real-time polymerase chain reaction (RT-PCR) was used to identify biovars of U.urealyticum in the semen.Results Sperm motion parameters including mean curvilinear velocity (VCL), mean straight-line velocity (VSL), mean average path (VAP) , and mean beat Cross frequency (BCF) showed significant difference between infertile and normal group (P ＜0.05).Biovar Ⅱ infected men displayed significant increase in leukocyte counts (P ＜ 0.001).When compared to uninfected group,the sperm motion parameters showed significant decreases of VCL, VSL, VAP, mean percentage lineality (LIN), and swing(WOB) in biovar Ⅱ (P ＜ 0.05).Conclusions Biovar Ⅱ has closer relationship with leukocyte elevation and sperm motion impairment.