中华胰腺病杂志
中華胰腺病雜誌
중화이선병잡지
Chinese Journal of Pancreatology
2015年
5期
306-309
,共4页
彭兰%潘涛%赵军%赵平武%李波
彭蘭%潘濤%趙軍%趙平武%李波
팽란%반도%조군%조평무%리파
胰腺炎,急性坏死性%肠道营养%Toll样受体4
胰腺炎,急性壞死性%腸道營養%Toll樣受體4
이선염,급성배사성%장도영양%Toll양수체4
Pancreatitis%acute necrotizing%Enteral nutrition%Toll-like receptor 4
目的 探讨早期肠内营养对急性坏死性胰腺炎( ANP)大鼠肠黏膜TLR4信号通路的作用及机制. 方法 将60只SD大鼠按数字表法随机分成对照组、肠外营养组( TPN组)、肠内营养组( EEN组). 造模后1d检测血清淀粉酶活性. 肠外营养、肠内营养持续给予5d后处死大鼠,取血、胰腺及结肠组织. 采用ELISA法检测血清IL-6、TNF-α水平;HE染色观察胰腺病理改变;蛋白质印迹法检测大鼠结肠组织TLR4 NF-κB 表达. 结果 对照组、TPN组、EEN 组大鼠造模后5 d的死亡率分别为0、50%、25%;血清淀粉酶活性分别为(744 ±41)、(3 278 ±219)、(2 227 ±169) U/L;TNF-α水平为(81.57 ± 18 .25 )、( 465 .72 ±42 .47 )、( 223 .21 ±29 .94 ) ng/L;IL-6 为( 362 .83 ±41 .32 )、( 932 .46 ±57 .21 )、(628.62 ±142.24)ng/L;胰腺病理评分为(0.91 ±0.15)、(11.1 ±0.61)、(6.9 ±0.62)分;结肠组织TLR4蛋白表达量为0.7506 ±0.003、1.3404 ±0.004、0.9544 ±0.004;NF-κB蛋白表达量为1.33 ±0.50、6.92 ±1.06、2.93 ±0.89. TPN组、EEN组均显著高于对照组,EEN组又显著低于TPN组,差异均有统计学意义(P值均<0.05). 结论 早期肠内营养能抑制ANP大鼠肠道TLR4及NF-κB信号通路,下调血清TNF-α、IL-6水平,减轻胰腺炎症反应,降低死亡率.
目的 探討早期腸內營養對急性壞死性胰腺炎( ANP)大鼠腸黏膜TLR4信號通路的作用及機製. 方法 將60隻SD大鼠按數字錶法隨機分成對照組、腸外營養組( TPN組)、腸內營養組( EEN組). 造模後1d檢測血清澱粉酶活性. 腸外營養、腸內營養持續給予5d後處死大鼠,取血、胰腺及結腸組織. 採用ELISA法檢測血清IL-6、TNF-α水平;HE染色觀察胰腺病理改變;蛋白質印跡法檢測大鼠結腸組織TLR4 NF-κB 錶達. 結果 對照組、TPN組、EEN 組大鼠造模後5 d的死亡率分彆為0、50%、25%;血清澱粉酶活性分彆為(744 ±41)、(3 278 ±219)、(2 227 ±169) U/L;TNF-α水平為(81.57 ± 18 .25 )、( 465 .72 ±42 .47 )、( 223 .21 ±29 .94 ) ng/L;IL-6 為( 362 .83 ±41 .32 )、( 932 .46 ±57 .21 )、(628.62 ±142.24)ng/L;胰腺病理評分為(0.91 ±0.15)、(11.1 ±0.61)、(6.9 ±0.62)分;結腸組織TLR4蛋白錶達量為0.7506 ±0.003、1.3404 ±0.004、0.9544 ±0.004;NF-κB蛋白錶達量為1.33 ±0.50、6.92 ±1.06、2.93 ±0.89. TPN組、EEN組均顯著高于對照組,EEN組又顯著低于TPN組,差異均有統計學意義(P值均<0.05). 結論 早期腸內營養能抑製ANP大鼠腸道TLR4及NF-κB信號通路,下調血清TNF-α、IL-6水平,減輕胰腺炎癥反應,降低死亡率.
목적 탐토조기장내영양대급성배사성이선염( ANP)대서장점막TLR4신호통로적작용급궤제. 방법 장60지SD대서안수자표법수궤분성대조조、장외영양조( TPN조)、장내영양조( EEN조). 조모후1d검측혈청정분매활성. 장외영양、장내영양지속급여5d후처사대서,취혈、이선급결장조직. 채용ELISA법검측혈청IL-6、TNF-α수평;HE염색관찰이선병리개변;단백질인적법검측대서결장조직TLR4 NF-κB 표체. 결과 대조조、TPN조、EEN 조대서조모후5 d적사망솔분별위0、50%、25%;혈청정분매활성분별위(744 ±41)、(3 278 ±219)、(2 227 ±169) U/L;TNF-α수평위(81.57 ± 18 .25 )、( 465 .72 ±42 .47 )、( 223 .21 ±29 .94 ) ng/L;IL-6 위( 362 .83 ±41 .32 )、( 932 .46 ±57 .21 )、(628.62 ±142.24)ng/L;이선병리평분위(0.91 ±0.15)、(11.1 ±0.61)、(6.9 ±0.62)분;결장조직TLR4단백표체량위0.7506 ±0.003、1.3404 ±0.004、0.9544 ±0.004;NF-κB단백표체량위1.33 ±0.50、6.92 ±1.06、2.93 ±0.89. TPN조、EEN조균현저고우대조조,EEN조우현저저우TPN조,차이균유통계학의의(P치균<0.05). 결론 조기장내영양능억제ANP대서장도TLR4급NF-κB신호통로,하조혈청TNF-α、IL-6수평,감경이선염증반응,강저사망솔.
Objective To investigate the role of early enteral nutrition on TLR 4 signaling pathway in rats with acute necrotizing pancreatitis (ANP).Methods Sixty SD rats were randomly divided into three groups:sham operation group ( SO group ) , total parenteral nutrition group ( TPN ) , early enteral nutrition group ( EEN ) .One day after ANP model induction , the serum level of amylase was measured .Nutrient solution was given for five days , then the rats were sacrificed , and the blood , pancreas and colon tissue were collected.The serum levels of IL-6, TNF-αwere detected by ELISA .Pathologic changes of pancreas were observed by HE staining.The intestinal TLR4, NF-κB expression was measured by Western blot .Results Mortality rates of SO group, TPN group, EEN group were 0, 50%, 25%, respectively; the serum levels of amylase were (744 ±41), (3 278 ±219), (2 227 ±169) U/L, respectively;the serum levels of TNF-αwere (81.57 ±18.25), (465.72 ±42.47), (223.21 ±29.94)ng/L, respectively; the serum levels of IL-6 were (362.83 ±41.32), (932.46 ±57.21), (628.62 ±142.24) ng/L, respectively; the pancreatic pathologic scores were (0.91 ±0.15), (11.1 ±0.61), (6.9 ±0.62);the intestinal TLR4 expressions were 0.7506 ± 0.003, 1.3404 ±0.004, 0.9544 ±0.004;the intestinal NF-κB expressions were 1.33 ±0.50, 6.92 ±1.06,2.93 ±0.89.The values of TPN and EEN group were significantly higher than those of SO group (P<0.05). The values of EEN group were significantly lower than those of TPN group (P<0.05).Conclusions EEN can inhibit TLR4 and NF-κB signal pathway in gut , then reduce IL-6 and TNF-αexpression and relieve inflammatory reaction of ANP , finally decrease the mortality of ANP .
