CAJ | 학술논문

目的：探索 miR-125a-5P 在髓母细胞瘤表皮生长因子受体（EGFR）信号通路中的调控机制。方法利用 TargetScan 和 Sanger 软件在 EGFR 信号通路中预测 miR-125a-5P 的潜在靶点，脂质体介导细胞基因转染，共分3组：对照组、无义组和 miR-125a-5P 组。荧光素酶实验验证 miR-125a-5P 与细胞周期蛋白依赖性激酶抑制剂-2B（CDKN2B）、E2F 转录因子3（E2F3）、丝裂原活化蛋白激酶-14（MAPK14）和生长因子受体结合蛋白-10（GRB10）间的靶矢关系；转染髓母细胞瘤 D341细胞 miR-125a-5p 寡核苷酸，反转录聚合酶链反应检测D341细胞中 miR-125a-5p 的表述水平，噻唑蓝法绘制细胞生长曲线，Transwell 实验检测瘤细胞迁移能力， Western blot 法检测 GRB10、EGFR、磷脂酰肌醇-3-羟基激酶（PI3K）和 Ras 的蛋白表达水平。结果荧光素酶实验结果显示，GRB10是所检测基因中唯一的 miR-125a-5P 靶基因；获得 miR-125a-5P 转染后 D341细胞增殖速度减慢。miR-125a-5P 组的细胞迁移率与对照组[（38.16±7.47）％]和无义组[（36.79±8.94）％]比较， miR-125a-5P 组最低[（13.59±4.41）％]，3组间比较有统计学差异（χ2＝11.495，P 约0.05）。miR-125a-5P 转染可致 EGFR 蛋白表达水平升高1.67倍，GRB10、PI3K 和 Ras 的水平分别降低至23％、61％和42％。结论miR-125a-5P 通过靶向沉默GRB10的表达抑制髓母细胞瘤EGFR 下游信号通路，发挥肿瘤生长抑制作用。
목적：탐색 miR-125a-5P 재수모세포류표피생장인자수체（EGFR）신호통로중적조공궤제。방법이용 TargetScan 화 Sanger 연건재 EGFR 신호통로중예측 miR-125a-5P 적잠재파점，지질체개도세포기인전염，공분3조：대조조、무의조화 miR-125a-5P 조。형광소매실험험증 miR-125a-5P 여세포주기단백의뢰성격매억제제-2B（CDKN2B）、E2F 전록인자3（E2F3）、사렬원활화단백격매-14（MAPK14）화생장인자수체결합단백-10（GRB10）간적파시관계；전염수모세포류 D341세포 miR-125a-5p 과핵감산，반전록취합매련반응검측D341세포중 miR-125a-5p 적표술수평，새서람법회제세포생장곡선，Transwell 실험검측류세포천이능력， Western blot 법검측 GRB10、EGFR、린지선기순-3-간기격매（PI3K）화 Ras 적단백표체수평。결과형광소매실험결과현시，GRB10시소검측기인중유일적 miR-125a-5P 파기인；획득 miR-125a-5P 전염후 D341세포증식속도감만。miR-125a-5P 조적세포천이솔여대조조[（38.16±7.47）％]화무의조[（36.79±8.94）％]비교， miR-125a-5P 조최저[（13.59±4.41）％]，3조간비교유통계학차이（χ2＝11.495，P 약0.05）。miR-125a-5P 전염가치 EGFR 단백표체수평승고1.67배，GRB10、PI3K 화 Ras 적수평분별강저지23％、61％화42％。결론miR-125a-5P 통과파향침묵GRB10적표체억제수모세포류EGFR 하유신호통로，발휘종류생장억제작용。
Objective To explore the regulation mechanism for miR - 125a - 5P in epidermal growth factor receptor(EGFR)signaling pathway in medulloblastoma. Methods The potential targets of miR - 125a - 5P in the EGFR signaling pathway were predicted by TargetScan and Sanger software,there were 3 groups:control group,non -sense group and miR - 125a - 5P group. Their relationship,between miR - 125a - 5P and cyclin - dependent kinase in-hibitor 2B( CDKN2B),E2F transcription factor 3( E2F3),mitogen - activated protein kinase 14( MAPK14)and growth factor receptor - bound protein 10(GRB10),were tested by luciferase experiments. After miR - 125a - 5P oligo-nucleotide was transfected to D341 cells,miR - 125a - 5P level was detected by reverse transcription polymerase chain reaction. Then the thiazolyl blue tetrazolium bromide assay was used to draw the cell growth curves,and Transwell assay was used to detect cell migration ability. The expression levels of GRB10,EGFR,phosphatidylinositol 3 - kinase(PI3K) and Ras were tested by Western blot method. Results The results of luciferase experimental results showed that GRB10 was the only target gene of miR - 125a - 5P. After miR - 125a - 5P being transfected,the D341 cell prolifera-tion obviously declined markedly. Compared with control group[(38. 16 ± 7. 47)% ]and the non - sense group [(36. 79 ± 8. 94)% ],cell migration rate in the miR - 125a - 5P group was lowest[(13. 59 ± 4. 41)% ],and there was a significant difference among 3 groups(χ2 = 11. 495,P < 0. 05);in the miR - 125a - 5P group,the expression level of EGFR increased 1. 67 times,GRB10,PI3K and Ras levels were reduced to 23% ,61% and 42% . Conclusion miR - 125a - 5P can inhibit tumor growth by silenced GRB10 expression targeting EGFR downstream signaling pathways in medulloblastoma.