CAJ | 학술논문

背景：目前，尽管利用各种材料制备的组织工程化骨研究取得了一定进展，但均表现出诸如支架材料降解速度与新生骨组织形成速率不匹配、组织生长缓慢、降解代谢产物有毒性等缺陷。 　　目的：构建一种新型的仿骨结构诱导性骨组织工程支架材料，评价其物理化学及生物学性能。 　　方法：以壳聚糖包被淫羊藿苷制备微球，检测其体外缓释效果；将载药微球与胶原蛋白复合构建支架材料的管芯；将羟基磷灰石、聚己内酯与胶原蛋白依次以0∶3∶3、1∶3∶3、2∶3∶3、3∶3∶3的比例混合于六氟异丙醇中，通过静电纺丝技术依次电纺制得具有4层结构的支架材料外管；以1%京尼平将经嵌套的管芯与外管交联在一起。利用万能材料试验机、表面接触角仪、红外光谱、扫描电镜、吸水率、透气性、孔隙率、体外降解实验等对交联前后外管材料的结构和特征进行表征，并评价骨髓间充质干细胞与外管材料的生物相容性；Wistar大鼠皮下埋置实验进一步评价交联前后外管材料的组织相容性。 　　结果与结论：药物在管芯中具有良好的缓释效果；制备的骨组织工程支架材料具有良好的均一性，交联后外管材料的力学性能、吸水率、透气性均高于未交联组(P <0.05)，且体外降解速率显著低于未交联组(P <0.05)。苏木精-伊红染色显示骨髓间充质干细胞可良好贴附于交联前后的外管材料上；交联的外管材料植入 Wistar大鼠皮下后均无炎症反应。表明交联后诱导性骨组织工程支架材料具有良好的生物相容性及力学性能。
배경：목전，진관이용각충재료제비적조직공정화골연구취득료일정진전，단균표현출제여지가재료강해속도여신생골조직형성속솔불필배、조직생장완만、강해대사산물유독성등결함。 　　목적：구건일충신형적방골결구유도성골조직공정지가재료，평개기물이화학급생물학성능。 　　방법：이각취당포피음양곽감제비미구，검측기체외완석효과；장재약미구여효원단백복합구건지가재료적관심；장간기린회석、취기내지여효원단백의차이0∶3∶3、1∶3∶3、2∶3∶3、3∶3∶3적비례혼합우륙불이병순중，통과정전방사기술의차전방제득구유4층결구적지가재료외관；이1%경니평장경감투적관심여외관교련재일기。이용만능재료시험궤、표면접촉각의、홍외광보、소묘전경、흡수솔、투기성、공극솔、체외강해실험등대교련전후외관재료적결구화특정진행표정，병평개골수간충질간세포여외관재료적생물상용성；Wistar대서피하매치실험진일보평개교련전후외관재료적조직상용성。 　　결과여결론：약물재관심중구유량호적완석효과；제비적골조직공정지가재료구유량호적균일성，교련후외관재료적역학성능、흡수솔、투기성균고우미교련조(P <0.05)，차체외강해속솔현저저우미교련조(P <0.05)。소목정-이홍염색현시골수간충질간세포가량호첩부우교련전후적외관재료상；교련적외관재료식입 Wistar대서피하후균무염증반응。표명교련후유도성골조직공정지가재료구유량호적생물상용성급역학성능。
BACKGROUND:Although there is a certain progress in the preparation of tissue-engineered bone tissue using a variety of materials, some deficiencies have appeared such as mismatching between scaffold degradation rate and new bone formation rate, slow tissue growth, toxic metabolites. OBJECTIVE:To build a new type of inducible bone repair composite scaffold with bionic bone structurematerials and to evaluate its physicochemical and biological properties. METHODS: Icarin encapsulated by chitosan was used to prepare drug-loaded microspheres, and the drug release rate of the microspheres was detected. Chitosan microspheres were mixed with colagen to build the core part of scaffold materials. Hydroxyapatite (HA), polycaprolactone (PCL) and colagen were mixed in hexafluoride isopropanol (HFIP) to prepare the HA/PCL/colagen outer part of composite scaffold material at the rate of 0:3:3, 1:3:3, 2:3:3, 3:3:3. Each proportional electrospinning was used for one layer, and finaly the 4-layer outer tube of the scaffold was produced. The tube core and outer tube were crosslinked by 1% genipin. Universal material testing machine, surface contact angle meter, infrared spectroscopy, scanning electron microscope, water absorption, permeability, porosity,in vitro degradation tests for cross-linked and uncross-linked were used to observe the structure and characteristics of tubular materials. Bone marrow mesenchymal stem cels were seeded on the surface of cross-linked and uncross-linked bone repair materials to evaluate the biocompatibility of the scaffolds. Cross-linked and uncross-linked bone repair materials were implanted subcutaneously into Wistar rats to evaluate the histocompatibility of the scaffolds. RESULTS AND CONCLUSION:The drug in the scaffold had a suitable release; the bone scaffold material had good uniformity, and cross-linked scaffolds materials had better mechanical properties, water absorption and permeability than the uncross-linked(P < 0.05). The degradation rate of the cross-linked group was significantly lower than that of the uncross-linked group (P< 0.05). Hematoxylin-eosin staining showed that the bone marrow mesenchymal stem cels could adhere wel to the cross-linked and uncross-linked materials. No inflammatory reactions occurred after subcutaneous implantation of cross-linked and uncross-linked materials. These findings indicate that the cross-linked scaffold for inducible bone tissue engineering has good biocompatibility and mechanical properties.