国际流行病学传染病学杂志
國際流行病學傳染病學雜誌
국제류행병학전염병학잡지
International Journal of Epidemiology and Infectious Disease
2015年
5期
310-313
,共4页
蓝银苑%吴兴中%覃晓琳%黄进梅%薛耀华%曾维英%欧江丽%唐三梅%方铭恒
藍銀苑%吳興中%覃曉琳%黃進梅%薛耀華%曾維英%歐江麗%唐三梅%方銘恆
람은원%오흥중%담효림%황진매%설요화%증유영%구강려%당삼매%방명항
奈瑟球菌,淋病%多抗原序列分型%头孢菌素类%敏感性
奈瑟毬菌,淋病%多抗原序列分型%頭孢菌素類%敏感性
내슬구균,임병%다항원서렬분형%두포균소류%민감성
Neisseria gonorrhoeae%Multi-antigen sequence typing%Cephalosporins%Sensitivity
目的 了解广州市临床分离淋病奈瑟球菌 (NG) 的分子型别及其对头孢菌素类抗生素的敏感性. 方法 收集广东省皮肤性病防治中心临床分离的121株NG,用琼脂稀释法测定菌株对头孢曲松和头孢克肟的最小抑菌浓度(MIC),并进行NG多抗原测序分型(NG-MAST). 结果 未发现NG对头孢曲松和头孢克肟耐药的菌株,但它们的低敏率分别达到13.2%(16/121)和2.5%(3/121). 121株NG共有82个型别,其中ST1768共6株,ST2083、ST5062和ST10229各4株,ST1766、ST1866和ST10339各3株, 有12个ST族群包含2株菌株,其余63 个型别仅含1 株菌株,占76.8%(63/121). 对头孢曲松低敏的13 株菌株中,ST10339和ST10242 各2株,其余9株均为单一型别. 结论 121 株NG对头孢曲松、头孢克肟的敏感性较高. 菌株NG-MAST型别具有较高的多样性.
目的 瞭解廣州市臨床分離淋病奈瑟毬菌 (NG) 的分子型彆及其對頭孢菌素類抗生素的敏感性. 方法 收集廣東省皮膚性病防治中心臨床分離的121株NG,用瓊脂稀釋法測定菌株對頭孢麯鬆和頭孢剋肟的最小抑菌濃度(MIC),併進行NG多抗原測序分型(NG-MAST). 結果 未髮現NG對頭孢麯鬆和頭孢剋肟耐藥的菌株,但它們的低敏率分彆達到13.2%(16/121)和2.5%(3/121). 121株NG共有82箇型彆,其中ST1768共6株,ST2083、ST5062和ST10229各4株,ST1766、ST1866和ST10339各3株, 有12箇ST族群包含2株菌株,其餘63 箇型彆僅含1 株菌株,佔76.8%(63/121). 對頭孢麯鬆低敏的13 株菌株中,ST10339和ST10242 各2株,其餘9株均為單一型彆. 結論 121 株NG對頭孢麯鬆、頭孢剋肟的敏感性較高. 菌株NG-MAST型彆具有較高的多樣性.
목적 료해엄주시림상분리임병내슬구균 (NG) 적분자형별급기대두포균소류항생소적민감성. 방법 수집광동성피부성병방치중심림상분리적121주NG,용경지희석법측정균주대두포곡송화두포극우적최소억균농도(MIC),병진행NG다항원측서분형(NG-MAST). 결과 미발현NG대두포곡송화두포극우내약적균주,단타문적저민솔분별체도13.2%(16/121)화2.5%(3/121). 121주NG공유82개형별,기중ST1768공6주,ST2083、ST5062화ST10229각4주,ST1766、ST1866화ST10339각3주, 유12개ST족군포함2주균주,기여63 개형별부함1 주균주,점76.8%(63/121). 대두포곡송저민적13 주균주중,ST10339화ST10242 각2주,기여9주균위단일형별. 결론 121 주NG대두포곡송、두포극우적민감성교고. 균주NG-MAST형별구유교고적다양성.
Objective To understand the molecular types of clinical isolated Neisseria gonorrhoeae (NG) in Guangzhou, and to analyze the sensitivity to the cephalosporin antibiotics. Methods A total of 121 NG strains isolated from Guangzhou Skin Disease Prevention and Control Center were included in this study. The agar dilution method was used to determine the minimum inhibitory concentrations (MIC) to ceftriaxone and cefixime. DNA was extracted for NG multi-antigen sequence typing (NG-MAST). Results The drug-resistance analysis showed that none of the strains appeared to be resistant to ceftriaxone and cefixime. However, the low sensitivity rates were 13.2%(16/121) and 2.5%(3/121), respectively. A total of 82 genotypes were identified among the 121 isolates. The most prevalent genotype was ST1768 (n=6) in NG strains isolated, followed by ST2083 (n=4), ST5062 (n=4), ST10229 (n=4), ST1766 (n=3), ST1866 (n=3), and ST10339 (n=3). There were 12 genotypes containing 2 NG strains, and 63 genotypes [76.8%(63/121) ] containing only one NG strain. Among the 13 NG strains with low sensitivity to ceftriaxone , there were 2 strains of ST10339 and 2 strains of ST10242 , and the rest were single genotype. Conclusions The 121 NG strains have relatively high sensitivity to ceftriaxone and cefixime. The genotypes of these NG-MAST strains are high diversity.
