CAJ | 학술논문

目的了解宁波地区诺如病毒(又称诺罗病毒)急性胃肠炎暴发疫情的病毒基因型,为预测病毒流行趋势提供依据. 方法收集2014年宁波市12 起急性胃肠炎暴发疫情的病例样本及相关流行病学资料, 所有样本用荧光RT-PCR方法检测诺如病毒和轮状病毒核酸. 诺如病毒核酸阳性样本进一步扩增病毒VP1基因片段,进行序列比对及遗传进化分析. 结果从12起急性胃肠炎暴发疫情中查明9起疫情病原为GⅠ.2、GⅡ.6 和 GⅡ.17型诺如病毒,其中6起由GⅡ.17型诺如病毒所致疫情均发生于12月份,波及5所学校和1所养老院. 序列比对和进化分析显示,宁波地区流行的GⅡ.17型诺如病毒与近期周边国家和地区分离到的病毒序列(JP/2014/Kawasaki323和13-BH-1/2013)同源性高,核苷酸差异均仅为2.6%. 结论 GⅡ.17型诺如病毒是2014年宁波地区诺如病毒型胃肠炎暴发的主要基因型. 与既往流行性特征相比,流行强度显著改变.
목적 료해저파지구낙여병독(우칭낙라병독)급성위장염폭발역정적병독기인형,위예측병독류행추세제공의거. 방법 수집2014년저파시12 기급성위장염폭발역정적병례양본급상관류행병학자료, 소유양본용형광RT-PCR방법검측낙여병독화륜상병독핵산. 낙여병독핵산양성양본진일보확증병독VP1기인편단,진행서렬비대급유전진화분석. 결과 종12기급성위장염폭발역정중사명9기역정병원위GⅠ.2、GⅡ.6 화 GⅡ.17형낙여병독,기중6기유GⅡ.17형낙여병독소치역정균발생우12월빈,파급5소학교화1소양로원. 서렬비대화진화분석현시,저파지구류행적GⅡ.17형낙여병독여근기주변국가화지구분리도적병독서렬(JP/2014/Kawasaki323화13-BH-1/2013)동원성고,핵감산차이균부위2.6%. 결론 GⅡ.17형낙여병독시2014년저파지구낙여병독형위장염폭발적주요기인형. 여기왕류행성특정상비,류행강도현저개변.
Objective To understand the genotype of the acute gastroenteritis outbreaks caused by norovirus (NoVs) in Ningbo, and to provide basis for the prevalence of the virus. Methods The epidemiologic data and specimens of 12 gastroenteritis outbreaks in Ningbo in 2014 were collected . All specimens were detected for NoVs and rotavirus by real-time RT-PCR. Sequence alignment and phylogenetic analysis were performed after amplification and sequencing Vp1 gene of NoVs. Results In 12 outbreaks, there were 9 outbreaks caused by GⅠ.2, GⅡ.6 and GⅡ.17 NoVs. Among them, 6 outbreaks caused by genotype GⅡ.17 NoV occurred in December, and involved 5 schools and 1 nursing home. The result of sequence alignment and phylogenetic analysis showed GⅡ.17 NoV, which was prevalent in Ningbo had high homology with those NoVs recently found in the neighboring countries and areas (JP/2014/Kawasaki323 and 13-BH-1/2013), and the nucleotide differences were both only 2.6%. Conclusions The GⅡ. 17 NoV is the predominant genotype of the acute gastroenteritis outbreaks caused by NoVs in Ningbo , 2014. Compared to the past epidemic characteristics , the epidemic intensity changes obviously.