CAJ | 학술논문

目的：探讨影响宫内调节性 T 淋巴细胞（Treg）分化的可能因素。方法收集200例新生儿脐带血，高分辨溶解曲线分析荧光定量法检测 Foxp3特异性甲基化区域（ TSDR）去甲基化水平，酶联免疫吸附（ELISA）法检测脐带血二氢二醇环氧苯并芘（BPDE）-DNA 加合物及白细胞介素4（IL-4）水平，问卷调查随访至出生1.0~1.5年，询问婴幼儿是否反复喘息和顽固性湿疹，并询问父母是否患哮喘等过敏性疾病。结果（1）喘息组[（0.48±0.05）％]和湿疹组[（0.76±0.05）％]Treg 数量明显低于无症状组[（1.14±0.08）％]（t ＝2.62、2.83，P 均﹤0.05）。父母特应性组 Treg 数量显著低于非特应性组 Treg 数量（P ﹤0.05）；特应性组发生喘息和湿疹婴幼儿 Treg 数量显著低于非特应性组，但非特异性组仍低于无症状组（ P ﹤0.05）。（2）喘息组[（236.30±6.59）ng/ L]和湿疹组[（173.40±7.38）ng/ L]BPDE-DNA 加合物水平显著高于无症状组[（111.01±3.36）ng/ L]（t ＝10.35、6.53，P 均﹤0.05）；特应性组中发生喘息和湿疹婴幼儿 BPDE-DNA 加合物水平低于非特应性组（P ﹤0.05）。（3）喘息组和湿疹组脐带血 IL-4水平明显高于无症状组（P ﹤0.05）。结论宫内遗传因素和BPDE-DNA 加合物可影响Treg 分化，Treg 分化相对不足可能是出生后婴幼儿过敏性疾病形成的原因之一。
목적：탐토영향궁내조절성 T 림파세포（Treg）분화적가능인소。방법수집200례신생인제대혈，고분변용해곡선분석형광정량법검측 Foxp3특이성갑기화구역（ TSDR）거갑기화수평，매련면역흡부（ELISA）법검측제대혈이경이순배양분병비（BPDE）-DNA 가합물급백세포개소4（IL-4）수평，문권조사수방지출생1.0~1.5년，순문영유인시부반복천식화완고성습진，병순문부모시부환효천등과민성질병。결과（1）천식조[（0.48±0.05）％]화습진조[（0.76±0.05）％]Treg 수량명현저우무증상조[（1.14±0.08）％]（t ＝2.62、2.83，P 균﹤0.05）。부모특응성조 Treg 수량현저저우비특응성조 Treg 수량（P ﹤0.05）；특응성조발생천식화습진영유인 Treg 수량현저저우비특응성조，단비특이성조잉저우무증상조（ P ﹤0.05）。（2）천식조[（236.30±6.59）ng/ L]화습진조[（173.40±7.38）ng/ L]BPDE-DNA 가합물수평현저고우무증상조[（111.01±3.36）ng/ L]（t ＝10.35、6.53，P 균﹤0.05）；특응성조중발생천식화습진영유인 BPDE-DNA 가합물수평저우비특응성조（P ﹤0.05）。（3）천식조화습진조제대혈 IL-4수평명현고우무증상조（P ﹤0.05）。결론궁내유전인소화BPDE-DNA 가합물가영향Treg 분화，Treg 분화상대불족가능시출생후영유인과민성질병형성적원인지일。
Objective To investigate the possible factors for differentiation affecting of neonatal regulatory T cells(Treg). Methods Umbilical cord blood was collected from 200 newborns. Treg number was detected by DNA demethylation in the Foxp3 of Treg - cell - specific demethylatedregion(TSDR)based on high resolution melting anal-ysis(HRMA),concentrations of 7,8 - dihydroxy - 9,10 - epoxy - benzo(a)pyrene(BPDE - DNA)adducts and interleukin - 4( IL - 4)in the supernatants of cord blood by enzyme - linked immunosorbent assay( ELISA),and follow - up questionnaires were carried out till 1. 0 - 1. 5 years,for recurrent wheezing or stubborn eczema in infants and related information on parental history of atopic diseases. Results (1)In wheezing group[(0. 48 ± 0. 05)% ]and ec-zema group[(0. 76 ± 0. 05)% ],the number of Tregs was significantly lower compared with that of the asymptomatic group[(1. 14 ± 0. 08)% ](t = 2. 62,2. 83,all P ﹤ 0. 05);the number of Treg in parental history of atopic group was significantly lower than that of the non - atopic group(P ﹤ 0. 05);but the Treg numbers in the non - atopic group was still lower than that of the asymptomatic group(P ﹤ 0. 05).(2)The concentrations of BPDE - DNA adducts in the wheezing group[(236. 30 ± 6. 59)ng/ L]and the eczema group[(173. 40 ± 7. 38)ng/ L]were higher than those of the asymptomatic group[(111. 01 ± 3. 36)ng/ L](t = 10. 35,6. 53,all P ﹤ 0. 05),while BPDE - DNA adduct concen-trations in the atopic group with parental history of wheezing or eczema in infants were lower than those of the non -atopic group(P ﹤ 0. 05).(3)The concentrations of IL - 4 in the wheezing or eczema group in the supernatants of cord blood was higher than the asymptomatic group(P ﹤ 0. 05). Conclusions Neonatal genetic factors and BPDE - DNA adducts could affect Treg differentiation,which are probably the reasons for the formation of allergic diseases.