CAJ | 학술논문

目的：探讨我国中部地区汉族儿童中白细胞介素（IL）-4（rs2243250）及 IL-13（rs1800925）基因多态性与过敏性紫癜（HSP）及紫癜性肾炎（HSPN）的相关性，并比较 HSP 和 HSPN 血清中2种细胞因子的差异。方法采取病例对照的方法，分别收集383例患儿作为试验组，其中 HSP 患儿320例（HSP 组），HSPN 患儿63例（HSPN 组），409例儿童为健康对照组；利用限制性片段长度多态性聚合酶链反应（ PCR-RFLP）的方法测定2个基因多态性的分布；酶联免疫吸附实验（ELISA）测定血清中 IL-4、IL-13的水平，并利用 SPSS 16.0统计软件进行数据分析。结果1. IL-4基因的单核苷酸多态性（SNP）位点的多态性在健康对照组、HSP 及 HSPN 组间分布差异无统计学意义（P ﹥0.05）；IL-13基因的 SNP 位点的多态性与 HSP 相关（P ＝0.037），且 T 等位基因频率明显高于健康对照组（P ＝0.027）；试验组（HSP 组+ HSPN 组）中 TT 基因型比例与健康对照组比较差异无统计学意义（P ＝0.132）；HSPN 组与健康对照组比较差异无统计学意义（P ＝0.487），且 HSP 组与 HSPN 组比较差异亦无统计学意义（P ＝0.129）。2. IL-13基因 SNP 位点多态性与血清中 IL-13水平相关，TT 基因型的患儿血清中水平高于其他基因型的患儿（P ﹤0.05）；IL-4基因的3种基因型之间血清中 IL-4的水平差异无统计学意义（P ﹥0.05）。3. HSPN 组 IL-13的水平高于 HSP 组（P ﹤0.05），而血清中 IL-4的水平在2组间差异无统计学意义。结论 IL-13基因的 SNP 位点（rs1800925）的多态性与 HSP 相关，且该 SNP 位点多态性影响血清中 IL-13的水平；HSPN 患儿血清 IL-13的水平高于 HSP 组，IL-13升高可能是 HSP 发展为 HSPN 的危险因素。
목적：탐토아국중부지구한족인동중백세포개소（IL）-4（rs2243250）급 IL-13（rs1800925）기인다태성여과민성자전（HSP）급자전성신염（HSPN）적상관성，병비교 HSP 화 HSPN 혈청중2충세포인자적차이。방법채취병례대조적방법，분별수집383례환인작위시험조，기중 HSP 환인320례（HSP 조），HSPN 환인63례（HSPN 조），409례인동위건강대조조；이용한제성편단장도다태성취합매련반응（ PCR-RFLP）적방법측정2개기인다태성적분포；매련면역흡부실험（ELISA）측정혈청중 IL-4、IL-13적수평，병이용 SPSS 16.0통계연건진행수거분석。결과1. IL-4기인적단핵감산다태성（SNP）위점적다태성재건강대조조、HSP 급 HSPN 조간분포차이무통계학의의（P ﹥0.05）；IL-13기인적 SNP 위점적다태성여 HSP 상관（P ＝0.037），차 T 등위기인빈솔명현고우건강대조조（P ＝0.027）；시험조（HSP 조+ HSPN 조）중 TT 기인형비례여건강대조조비교차이무통계학의의（P ＝0.132）；HSPN 조여건강대조조비교차이무통계학의의（P ＝0.487），차 HSP 조여 HSPN 조비교차이역무통계학의의（P ＝0.129）。2. IL-13기인 SNP 위점다태성여혈청중 IL-13수평상관，TT 기인형적환인혈청중수평고우기타기인형적환인（P ﹤0.05）；IL-4기인적3충기인형지간혈청중 IL-4적수평차이무통계학의의（P ﹥0.05）。3. HSPN 조 IL-13적수평고우 HSP 조（P ﹤0.05），이혈청중 IL-4적수평재2조간차이무통계학의의。결론 IL-13기인적 SNP 위점（rs1800925）적다태성여 HSP 상관，차해 SNP 위점다태성영향혈청중 IL-13적수평；HSPN 환인혈청 IL-13적수평고우 HSP 조，IL-13승고가능시 HSP 발전위 HSPN 적위험인소。
Objective To investigate the association between 2 single nucleotide polymorphisms( SNPs) [rs2243250 in interleukin(IL)- 4 gene and rs1800925 in IL - 13 gene]in Henoch - Schonlein purpura(HSP)and Henoch - Schonlein purpura nephritis(HSPN)in Han Chinese children. The level of IL - 4 and IL - 13 in serum were compared between HSP and HSPN. Methods A case - control study was adopted in this research,and 383 children with HSP or HSPN were recruited in this study,409 normal children served as controls. The genotypes of 2 SNPs were detected by using polymerase chain reaction - restriction fragment length polymorphism(PCR - RFLP),and the serum levels of IL - 4 and IL - 13 in HSP and HSPN patients were detected by using enzyme linked immunosorbent assay (ELISA). All the data were analyzed by SPSS 16. 0 software. Results (1)There was no association(polymorphism of SNP rs2243250 in IL - 4 gene)in HSP group and HSPN group compared with the healthy control group(P ﹥ 0. 05);and the polymorphism of another SNP(rs1800925 in IL - 13 gene)was associated with HSP(P = 0. 037),and the fre-quency of T allele of the study group was higher than that of the healthy control group(P = 0. 027). But there was no difference between the study group(HSP group and HSPN group)and the healthy control group in frequency of TT genotype(P = 0. 132),and there was no association between HSPN group and healthy control group(P = 0. 487);also there was no association between polymorphism of this SNP in HSP group and HSPN group(P = 0. 129).(2)There was an association between polymorphism of IL - 13 gene and IL - 13 level in serum,patients with TT genotype had a higher serum IL - 13 level than any other genotypes(P ﹤ 0. 05),but there was no statistically significant difference in IL - 4 level between TT genotype and other genotypes(P ﹥ 0. 05).(3)There was an association between polymorphism of IL - 13 gene and IL - 13 level in serum patients with HSPN who had a higher level of serum IL - 13 than the patients with HSP( P ﹤ 0. 05),but there was no difference in serum IL - 4 level between HSP group and HSPN group. Conclusions The polymorphism of SNP(rs1800925 in IL - 13 gene)was associated with HSP group,and the poly-morphism of this SNP might affect the level of IL - 13 in serum. The patients with HSPN have a higher level of serum IL -13 than the patients with HSP,and high level of IL -13 may be a risk factor in the progression from HSP to HSPN.