CAJ | 학술논문

背景：组织工程心肌补片可以通过体内移植，使种子细胞准确到达梗死心肌区域并进行再生修复，达到改善心功能的治疗效果。 　　目的：回顾制备组织工程心肌补片的构建方法和策略，分析不同构建方法制备心肌补片的结构功能特点。 　　方法：由第一作者检索2000至2014年Web of science核心数据库、PudMed数据库，检索 　　主题词为“cardiac infarction；cel patch”。 　　结果与结论：制备组织工程心肌补片的方法和策略有：叠加单层细胞片获取复层细胞结构；将胶原凝胶等材料与细胞混合形成复层结构；在支架材料上种植细胞；使细胞齐向排列等。目前关于组织工程心肌补片的研究显示，将细胞片进行体内移植后，种子细胞在心肌梗死区域停留率、存活率与旁分泌作用等，在很大程度上影响细胞片对梗死心肌的再生修复作用。现有的细胞片构建方法中以温敏法构建技术最为成熟、研究使用最为广泛，与此同时，其他构建方法也可不同程度地提高种子细胞的增殖、分化及旁分泌能力，增加细胞片的力学强度与厚度以增加移植可操作性。研究人员常结合多种方法用于制备获取生物学及力学特性良好的组织工程心肌补片。
배경：조직공정심기보편가이통과체내이식，사충자세포준학도체경사심기구역병진행재생수복，체도개선심공능적치료효과。 　　목적：회고제비조직공정심기보편적구건방법화책략，분석불동구건방법제비심기보편적결구공능특점。 　　방법：유제일작자검색2000지2014년Web of science핵심수거고、PudMed수거고，검색 　　주제사위“cardiac infarction；cel patch”。 　　결과여결론：제비조직공정심기보편적방법화책략유：첩가단층세포편획취복층세포결구；장효원응효등재료여세포혼합형성복층결구；재지가재료상충식세포；사세포제향배렬등。목전관우조직공정심기보편적연구현시，장세포편진행체내이식후，충자세포재심기경사구역정류솔、존활솔여방분비작용등，재흔대정도상영향세포편대경사심기적재생수복작용。현유적세포편구건방법중이온민법구건기술최위성숙、연구사용최위엄범，여차동시，기타구건방법야가불동정도지제고충자세포적증식、분화급방분비능력，증가세포편적역학강도여후도이증가이식가조작성。연구인원상결합다충방법용우제비획취생물학급역학특성량호적조직공정심기보편。
BACKGROUND:In recent years, a lot of studies have reported the feasibility and efficacy of transplanting seed celsvia tissue-engineered cardiac patches into specific infarcted myocardial area, helping regeneration of damaged heart tissue and improving heart functions. OBJECTIVE:To review the methods and strategies of fabricating tissue-engineered cardiac patches and to analyze the structure and functional characteristics of cardiac patches prepared by different methods. METHODS: The first author retrieved relevant articles about the fabrication methods of tissue engineered cardiac patches by searching PubMed and Web of science data bases. The key words were “cardiac infarction, cel death”. RESULTS AND CONCLUSION:The fabrication methods and strategies of tissue-engineered cardiac patches are as folows: (1) obtaining multi-layer cel sheets by overlapping monolayer cel sheets; (2) combing gelatin with cels to construct multi-layer cel sheets; (3) seeding cels on scaffolds; (4) homogeneous arrangement of cels. Recent studies about tissue-engineered cardiac patches have demonstrated that the biological activities of seed cels, such as retention, survival and paracrine effects, greatly influence the effect of cardiac patches on the repair and regeneration of infarcted myocardial tissues. Temperature-sensitive method is the most mature and wide-spread used constructing technique; besides, other strategies also successfuly improve the biological activities of seed cels to different extents, and they also increase the mechanical strength and thickness of cel sheets in order to have better operability. Tissue-engineered cardiac patches are usualy prepared by different combined strategies on purpose to obtain better biological and mechanical properties.