CAJ | 학술논문

背景：壳聚糖水凝胶具有良好的生物兼容性、生物可降解性及抗菌性等特点，具有促进组织愈合和成骨诱导的作用，作为支架材料载负生长因子可保证外源生长因子的缓慢高效释放。 　　目的：观察可注射性纳米壳聚糖骨形态发生蛋白2复合体促进大鼠牙周组织再生的效果。 　　方法：将54只Wistar大鼠随机均分为3组，建立第二磨牙慢性牙周炎模型，建模成功后，实验组于第二磨牙牙周内植入可注射性纳米壳聚糖骨形态发生蛋白2复合体，对照组于第二磨牙牙周内植入可注射性纳米壳聚糖凝胶，空白组牙周内不植入任何药物。术后3，6，9周，进行牙龈出血指数、探诊深度、松动度、X射线片及组织病理学切片观察。 　　结果与结论：实验组术后9周的探诊深度、松动度均低于对照组及空白组(P<0.05)。术后9周，实验组牙槽骨高度修复再生至根分叉处，骨小梁致密，分布均匀，可见大量牙骨质样结构、牙周韧带生成，新生牙槽骨致密且均匀分布在骨缺损区域，修复效果明显优于对照组及空白组。表明可注射性纳米壳聚糖骨形态发生蛋白2复合体具有抗炎和引导牙周组织再生的作用。
배경：각취당수응효구유량호적생물겸용성、생물가강해성급항균성등특점，구유촉진조직유합화성골유도적작용，작위지가재료재부생장인자가보증외원생장인자적완만고효석방。 　　목적：관찰가주사성납미각취당골형태발생단백2복합체촉진대서아주조직재생적효과。 　　방법：장54지Wistar대서수궤균분위3조，건립제이마아만성아주염모형，건모성공후，실험조우제이마아아주내식입가주사성납미각취당골형태발생단백2복합체，대조조우제이마아아주내식입가주사성납미각취당응효，공백조아주내불식입임하약물。술후3，6，9주，진행아간출혈지수、탐진심도、송동도、X사선편급조직병이학절편관찰。 　　결과여결론：실험조술후9주적탐진심도、송동도균저우대조조급공백조(P<0.05)。술후9주，실험조아조골고도수복재생지근분차처，골소량치밀，분포균균，가견대량아골질양결구、아주인대생성，신생아조골치밀차균균분포재골결손구역，수복효과명현우우대조조급공백조。표명가주사성납미각취당골형태발생단백2복합체구유항염화인도아주조직재생적작용。
BACKGROUND:Chitosan hydrogel has good biocompatibility, biodegradability and antibacterial property, which can promote tissue healing and induce bone formation. As a scaffold carrying growth factors, it can ensure the efficient and slow release of exogenous growth factors. OBJECTIVE: To observe the effect of injectable nano/chitosan/bone morphogenetic protein-2 composite to promote periodontal tissue regeneration in rats. METHODS:Fifty-four Wistar rats were randomized into three groups, and then chronic periodontitis model of the second molar was established. After modeling, injectable nano/chitosan/bone morphogenetic protein-2 composite was implanted into the periodontal tissue of the second molar in the experimental group; injectable nano/chitosan hydrogel was implanted in the control grouop; and nothing was implanted in the blank group. At 3, 6, 9 weeks after surgery, gingival bleeding index, probing depth, and tooth mobility were detected. X-ray and histopathological observations were carried out. RESULTS AND CONCLUSION:At 9 weeks after surgery, the probing depth and tooth mobility were both lower in the experimental group than the other two groups (P < 0.05). In the experimental group, the alveolar bone height was restored at the root bifurcations, bone trabeculae were arranged densely and evenly, the newly formed periodontal ligament and alveolar bone tissues were dense and equaly distributed in the bone defect area at 9 weeks after surgery, indicating a better restoration effect than the control and blank groups. These findings suggest that the injectable nano/chitosan/bone morphogenetic protein-2 composite has an anti-inflammatory role and can guide periodontal tissue regeneration.