CAJ | 학술논문

背景：纳米银具有高效、广谱抗菌性及不易产生耐药性等优点，已成为目前抗菌材料的研究热点，但目前尚不明确其抗菌的确切机制。 　　目的：研究纳米银抵抗细菌生长的机制。 　　方法：采用抑菌环实验检测Ti、TiO2及载纳米银TiO2对单克隆大肠杆菌和金黄色葡萄球菌的抑制作用；将大肠杆菌接种于LB液体培养基中，分别加入0，5，10 mg/L的纳米银溶液，检测24 h内的菌液A值，进行琼脂糖凝胶电泳，分析细菌DNA的变化，应用Annexin V和PI双染色，采用流式细胞术检测细菌凋亡。 　　结果与结论：Ti 和 TiO2对金黄色葡萄球菌和大肠杆菌无明显抑制现象，在 TiO2管载纳米银材料片周围出现了明显抑菌环。加入纳米银溶液后，大肠杆菌菌液A值明显降低，并且随着加入纳米银质量浓度的增加，降低趋势更明显。加入纳米银溶液后，大肠杆菌的DNA含量明显减少，并且减少程度随加入纳米银溶液质量浓度的增加而增加。加入纳米银后，细菌Annexin V阳性率增加，并且这种增加趋势与加入纳米银的质量浓度呈正比。表明纳米银可通过促进细菌凋亡这一机制来影响细菌生长。
배경：납미은구유고효、엄보항균성급불역산생내약성등우점，이성위목전항균재료적연구열점，단목전상불명학기항균적학절궤제。 　　목적：연구납미은저항세균생장적궤제。 　　방법：채용억균배실험검측Ti、TiO2급재납미은TiO2대단극륭대장간균화금황색포도구균적억제작용；장대장간균접충우LB액체배양기중，분별가입0，5，10 mg/L적납미은용액，검측24 h내적균액A치，진행경지당응효전영，분석세균DNA적변화，응용Annexin V화PI쌍염색，채용류식세포술검측세균조망。 　　결과여결론：Ti 화 TiO2대금황색포도구균화대장간균무명현억제현상，재 TiO2관재납미은재료편주위출현료명현억균배。가입납미은용액후，대장간균균액A치명현강저，병차수착가입납미은질량농도적증가，강저추세경명현。가입납미은용액후，대장간균적DNA함량명현감소，병차감소정도수가입납미은용액질량농도적증가이증가。가입납미은후，세균Annexin V양성솔증가，병차저충증가추세여가입납미은적질량농도정정비。표명납미은가통과촉진세균조망저일궤제래영향세균생장。
BACKGROUND:Silver nanoparticles (AgNPs) show strong antibacterial effect and are not easy to have drug resistance. But the antibacterial mechanisms of AgNPs have not been wel developed. OBJECTIVE:To explain the antibacterial mechanisms of AgNPs. METHODS:We investigated the influence of Ti, TiO2 and TiO2 containing AgNPs onEscherichia coliand Staphylococcus aureus by bacterial inhibition ring test. Escherichia coli was cultured in LB liquid medium with 0, 5, 10 mg/L AgNPs. We measured the absorbance value of bacterial culture. DNA gel electrophoresis was used to study the effect of AgNPs onEscherichia coliDNA. Then we researched the character of apoptosis on Escherichia coli by Annexin V and PI staining, using flow cytometry. RESULTS AND CONCLUSION:The inhibiting effect of Ti and TiO2 onEscherichia coliandStaphylococcus aureus was not obvious. But the inhibition rings of TiO2 containing AgNPs to bacteria appeared. The absorbance value of Escherichia coliculture was reduced whenEscherichia coliwas co-cultured with AgNPs. And this decrease tendency was in direct proportion with AgNPs concentration. AgNPs reduced the amount of DNA of Escherichia coli and this tendency was directly proportional with AgNPs concentration. TheEscherichia coli apoptosis rate induced by AgNPs was increased and this tendency was positively correlated to the AgNPs concentration. These results indicate that AgNPs can induce bacterial apoptosis to influence the growth of bacteria.