山东医药
山東醫藥
산동의약
Shandong Medical Journal
2015年
41期
10-12
,共3页
李云%宋银宏%吕云波%秦琴
李雲%宋銀宏%呂雲波%秦琴
리운%송은굉%려운파%진금
心绞痛,不稳定型%血液标本%保存时间%miRNAs
心絞痛,不穩定型%血液標本%保存時間%miRNAs
심교통,불은정형%혈액표본%보존시간%miRNAs
angina%unstable%blood specimens%preserving time%miRNAs
目的 探讨血液标本保存时间对不稳定型心绞痛患者血浆中miRNAs ( miRNA-21、miRNA-24、miRNA-126、miRNA-146、miRNA-223)检测水平的影响,确定其检测时间窗. 方法 选取6例接受冠脉造影检查的不稳定型心绞痛患者,造影时抽取外周动脉血分装于6支EDTA抗凝管中,每管2 mL;其中3管立即离心分离血浆,4℃冰箱保存;余3管全血放入4 ℃冰箱保存. 采用实时荧光定量聚合酶链反应方法,3管血浆分别于即刻(0 h)、24 h、1周后检测miRNAs水平,3管全血分别于4、24 h及1周后离心获得血浆再检测miRNAs水平,比较各时间段血浆miRNAs水平的差异.结果 ①与0h时比较,血液标本即刻离心保存24h后血浆miRNA-21、miRNA-24、miRNA-126、miRNA-223水平下降(P均<0.05),1周后血浆5种miRNA水平均明显下降(P均<0.05). ②与0 h时比较,全血保存4 h后离心分离检测血浆miRNA-21水平下降(P<0.05),24 h后血浆5种miRNA水平均下降(P均<0.05). 结论 不稳定型心绞痛患者保存不同时间血浆中miRNAs稳定性不一;其中血浆miRNA-21稳定性最差,需取血后即刻检测.
目的 探討血液標本保存時間對不穩定型心絞痛患者血漿中miRNAs ( miRNA-21、miRNA-24、miRNA-126、miRNA-146、miRNA-223)檢測水平的影響,確定其檢測時間窗. 方法 選取6例接受冠脈造影檢查的不穩定型心絞痛患者,造影時抽取外週動脈血分裝于6支EDTA抗凝管中,每管2 mL;其中3管立即離心分離血漿,4℃冰箱保存;餘3管全血放入4 ℃冰箱保存. 採用實時熒光定量聚閤酶鏈反應方法,3管血漿分彆于即刻(0 h)、24 h、1週後檢測miRNAs水平,3管全血分彆于4、24 h及1週後離心穫得血漿再檢測miRNAs水平,比較各時間段血漿miRNAs水平的差異.結果 ①與0h時比較,血液標本即刻離心保存24h後血漿miRNA-21、miRNA-24、miRNA-126、miRNA-223水平下降(P均<0.05),1週後血漿5種miRNA水平均明顯下降(P均<0.05). ②與0 h時比較,全血保存4 h後離心分離檢測血漿miRNA-21水平下降(P<0.05),24 h後血漿5種miRNA水平均下降(P均<0.05). 結論 不穩定型心絞痛患者保存不同時間血漿中miRNAs穩定性不一;其中血漿miRNA-21穩定性最差,需取血後即刻檢測.
목적 탐토혈액표본보존시간대불은정형심교통환자혈장중miRNAs ( miRNA-21、miRNA-24、miRNA-126、miRNA-146、miRNA-223)검측수평적영향,학정기검측시간창. 방법 선취6례접수관맥조영검사적불은정형심교통환자,조영시추취외주동맥혈분장우6지EDTA항응관중,매관2 mL;기중3관립즉리심분리혈장,4℃빙상보존;여3관전혈방입4 ℃빙상보존. 채용실시형광정량취합매련반응방법,3관혈장분별우즉각(0 h)、24 h、1주후검측miRNAs수평,3관전혈분별우4、24 h급1주후리심획득혈장재검측miRNAs수평,비교각시간단혈장miRNAs수평적차이.결과 ①여0h시비교,혈액표본즉각리심보존24h후혈장miRNA-21、miRNA-24、miRNA-126、miRNA-223수평하강(P균<0.05),1주후혈장5충miRNA수평균명현하강(P균<0.05). ②여0 h시비교,전혈보존4 h후리심분리검측혈장miRNA-21수평하강(P<0.05),24 h후혈장5충miRNA수평균하강(P균<0.05). 결론 불은정형심교통환자보존불동시간혈장중miRNAs은정성불일;기중혈장miRNA-21은정성최차,수취혈후즉각검측.
Objective To investigate the effect of blood-preserving time on the expression of miRNAs ( miRNA-21, miRNA-24, miRNA-126, miRNA-146 and miRNA-223 ) in the plasma of patients with unstable angina pectoris ( UAP) , and to make sure the time window for checking their expression in plasma clinically.Methods Six UAP patients who accepted coronary angiography were selected as subjects.The peripheral blood ( PB) samples of the pa-tients were collected in EDTA anticoagulant tubes and separated into 6 tubes (2 ML in each).During the six tubes, the plasma of 3 tubes were separated, and was preserved at 4℃, the other 3 tubes were preserved at 4 ℃.The levels of miRNAs were detected in the 3 tubes at the moment of centrifugation ( 0 h) , 24 h and one week later by real-time fluorescent quantitative PCR.The same procedures were conducted in the other 3 tubes of whole blood after the plasma wascollected.ThelevelsofplasmamiRNAswerecomparedatdifferenttimepoints.Results ①Theconcentrations of miRNA-21, miRNA-24, miRNA-126 and miRNA-223 in plasma were decreased significantly after being stored for 24 h as compared with the moment of 0 h(all P<0.05).The concentrations of all the 5 miRNAs in plasma were de-creased significantly after being stored for one week as compared with that of 24 h( P<0.05) .② The concentration of miRNA-21 in plasma was decreased significantly after being stored for 4 hours as compared with that of 0 h( P<0.05),and the concentrations of 5 miRNAs in plasma were decreased significantly after being stored for 24 h (P<0.05).Conclusion There are differences among the stabilities of different miRNAs in plasma of UAP patients, and miRNA-21 must be examined immediately owing to its poor stability.
