中华放射肿瘤学杂志
中華放射腫瘤學雜誌
중화방사종류학잡지
Chinese Journal of Radiation Oncology
2015年
6期
719-723
,共5页
李倩雯%李珂%张盛%杨天洋%周瑜%李振宇%周方正%马虹%董晓荣%刘莉%伍钢%孟睿
李倩雯%李珂%張盛%楊天洋%週瑜%李振宇%週方正%馬虹%董曉榮%劉莉%伍鋼%孟睿
리천문%리가%장성%양천양%주유%리진우%주방정%마홍%동효영%류리%오강%맹예
肺癌细胞系%放射增敏%蛋白激酶CK2%醌茜素
肺癌細胞繫%放射增敏%蛋白激酶CK2%醌茜素
폐암세포계%방사증민%단백격매CK2%곤천소
Lung neoplasms cell line%Radiosensitivity%Protein Kinase CK2%Quinalizarin
目的：探讨蛋白激酶CK2抑制剂对肺癌细胞系放射敏感性的影响。方法通过蛋白印迹法检测蛋白激酶CK2α、β亚基在不同肺癌细胞系中的表达情况。平板克隆形成试验检测CK2激酶抑制剂醌茜素对肺腺癌细胞A549和大细胞肺癌细胞H460对X线的放射敏感性影响。流式细胞术检测醌茜素与X线联合作用对A549、H460细胞凋亡及周期分布影响。组间比较采用方差分析和成组t检验。结果蛋白激酶CK2α、β亚基在对放射不敏感的A549、H1650、H460细胞中高表达，而在放射敏感的小细胞肺癌H446细胞中低表达。使用醌茜素预处理的A549、H460细胞存活分数（ SF）明显低于未处理组，25μmol／L的增敏比（ D0值比）分别为2．771、2．463。醌茜素可引起细胞凋亡增加，但X线联合醌茜素与单独醌茜素作用相比未增加A549细胞和H460细胞凋亡（ X线照射＋醌茜素：单独醌茜素，A549细胞P＝0．487和H460细胞P＝0．254），然而X线联合醌茜素与单独X线照射或醌茜素作用相比可明显引起其G2＋M期阻滞（ X线照射＋醌茜素：单独X线照射，A549细胞P＝0．000，H460细胞P＝0．0024；X线照射＋醌茜素：单独X线照射，A549细胞P＝0．000，H460细胞P＝0．000）。结论通过醌茜素抑制蛋白激酶CK2活性可增加NSCLC细胞的放射敏感性。
目的：探討蛋白激酶CK2抑製劑對肺癌細胞繫放射敏感性的影響。方法通過蛋白印跡法檢測蛋白激酶CK2α、β亞基在不同肺癌細胞繫中的錶達情況。平闆剋隆形成試驗檢測CK2激酶抑製劑醌茜素對肺腺癌細胞A549和大細胞肺癌細胞H460對X線的放射敏感性影響。流式細胞術檢測醌茜素與X線聯閤作用對A549、H460細胞凋亡及週期分佈影響。組間比較採用方差分析和成組t檢驗。結果蛋白激酶CK2α、β亞基在對放射不敏感的A549、H1650、H460細胞中高錶達，而在放射敏感的小細胞肺癌H446細胞中低錶達。使用醌茜素預處理的A549、H460細胞存活分數（ SF）明顯低于未處理組，25μmol／L的增敏比（ D0值比）分彆為2．771、2．463。醌茜素可引起細胞凋亡增加，但X線聯閤醌茜素與單獨醌茜素作用相比未增加A549細胞和H460細胞凋亡（ X線照射＋醌茜素：單獨醌茜素，A549細胞P＝0．487和H460細胞P＝0．254），然而X線聯閤醌茜素與單獨X線照射或醌茜素作用相比可明顯引起其G2＋M期阻滯（ X線照射＋醌茜素：單獨X線照射，A549細胞P＝0．000，H460細胞P＝0．0024；X線照射＋醌茜素：單獨X線照射，A549細胞P＝0．000，H460細胞P＝0．000）。結論通過醌茜素抑製蛋白激酶CK2活性可增加NSCLC細胞的放射敏感性。
목적：탐토단백격매CK2억제제대폐암세포계방사민감성적영향。방법통과단백인적법검측단백격매CK2α、β아기재불동폐암세포계중적표체정황。평판극륭형성시험검측CK2격매억제제곤천소대폐선암세포A549화대세포폐암세포H460대X선적방사민감성영향。류식세포술검측곤천소여X선연합작용대A549、H460세포조망급주기분포영향。조간비교채용방차분석화성조t검험。결과단백격매CK2α、β아기재대방사불민감적A549、H1650、H460세포중고표체，이재방사민감적소세포폐암H446세포중저표체。사용곤천소예처리적A549、H460세포존활분수（ SF）명현저우미처리조，25μmol／L적증민비（ D0치비）분별위2．771、2．463。곤천소가인기세포조망증가，단X선연합곤천소여단독곤천소작용상비미증가A549세포화H460세포조망（ X선조사＋곤천소：단독곤천소，A549세포P＝0．487화H460세포P＝0．254），연이X선연합곤천소여단독X선조사혹곤천소작용상비가명현인기기G2＋M기조체（ X선조사＋곤천소：단독X선조사，A549세포P＝0．000，H460세포P＝0．0024；X선조사＋곤천소：단독X선조사，A549세포P＝0．000，H460세포P＝0．000）。결론통과곤천소억제단백격매CK2활성가증가NSCLC세포적방사민감성。
Objective To evaluate the effect of an inhibitor of protein kinase CK2 on the radiosensitivity of human lung cancer cells. Methods The protein levels of CK2 α and β subunits in different lung cancer cell lines were measured by Western blot. Clonogenic assays were performed to assess the effect of a CK2 inhibitor, quinalizarin, on the radiosensitivity of lung adenocarcinoma A549 cells and large cell lung cancer H460 cells. The effects of the combination of quinalizarin and X?ray irradiation on the apoptosis and cell cycle of A549 and H460 cells were measured by flow cytometry. The differences between two groups were analyzed by analysis of variance and t?test. Results Western blot revealed that theαandβsubunits of CK2 were overexpressed in non?small cell lung cancer cells (A549,H460, and H1650 cells), which were considered insensitive to X?ray irradiation, whereas a lower expression of these two subunits were found in small cell lung cancer cells ( H446 cells) , which were sensitive to X?ray irradiation. The clonogenic assays showed that A549 and H460 cells pre?exposed to quinalizarin had a significantly lower survival fraction compared with the control group and had a sensitization enhancement ratio greater than 1. 0( D0 were 2. 771 and 2. 463 respectively) . The combination of quinalizarin and X?ray irradiation did not increase the apoptosis of A549 and H460 cells ( X?ray+Quinalizarin vs. Quinalizarin, A549, P=0. 487 and H460, P=0. 254) , but caused significant G2/M arrest compared with under X?ray irradiation only ( X?ray +Quinalizarin:X?ray, A549, P=0. 000;H460, P=0. 002 and X?ray+Quinalizarin:Quinalizarin, A549, P=0. 000;H460,P=0. 000) . Conclusions Quinalizarin, as a CK2 inhibitor, can increase the radiosensitivity of non?small cell lung cancer cells.
