CAJ | 학술논문

目的：探讨血浆miR－127－3p在乳腺癌患者中的表达及其临床意义。方法收集南通大学附属医院2013年10月至2014年1月收治的80例乳腺癌患者、70名良性乳腺肿瘤患者及70名健康体检者血浆标本，采用SYBR Green 实时荧光定量逆转录聚合酶链反应（ RT－PCR ）检测血浆中miR－127－3p含量，评价检测方法的线性、重复性及特异性，并对乳腺癌患者血浆miR－127－3p含量与癌胚抗原（CEA）、CA153浓度进行相关性分析。 Mann－Whitney检验分析血浆miR－127－3p的表达与乳腺癌患者临床病理特征的关系。结果成功建立血浆miR－127－3p的检测方法，乳腺癌患者血浆中miR－127－3p的相对表达量（RQ）14．158（5．424～16．465）显著高于乳腺良性肿瘤患者3．015（1．987～5．035）（P＝0．0006）和健康对照者2．375（1．173～4．370）（P＝0．0002）。而乳腺良性肿瘤患者与健康对照者之间，差异无统计学意义（ P＝0．143）。 miR－127－3p相对表达量与CA153浓度正相关性（R2＝0．457，P＝0．003）。 miR－127－3p ROC曲线下面积（AUC）为0．763，高于传统乳腺肿瘤标志物CEA和CA153。乳腺癌患者血浆miR－127－3p的表达水平在不同肿瘤直径、分化程度和TNM（ tumor node metastasis ）分期间差异无统计学意义（ P＞0．05）。结论 miR－127－3p在乳腺癌患者中高表达，可能是诊断乳腺癌患者的一个重要参考指标。（中华检验医学杂志，2015，38：682－685）
목적：탐토혈장miR－127－3p재유선암환자중적표체급기림상의의。방법수집남통대학부속의원2013년10월지2014년1월수치적80례유선암환자、70명량성유선종류환자급70명건강체검자혈장표본，채용SYBR Green 실시형광정량역전록취합매련반응（ RT－PCR ）검측혈장중miR－127－3p함량，평개검측방법적선성、중복성급특이성，병대유선암환자혈장miR－127－3p함량여암배항원（CEA）、CA153농도진행상관성분석。 Mann－Whitney검험분석혈장miR－127－3p적표체여유선암환자림상병리특정적관계。결과성공건립혈장miR－127－3p적검측방법，유선암환자혈장중miR－127－3p적상대표체량（RQ）14．158（5．424～16．465）현저고우유선량성종류환자3．015（1．987～5．035）（P＝0．0006）화건강대조자2．375（1．173～4．370）（P＝0．0002）。이유선량성종류환자여건강대조자지간，차이무통계학의의（ P＝0．143）。 miR－127－3p상대표체량여CA153농도정상관성（R2＝0．457，P＝0．003）。 miR－127－3p ROC곡선하면적（AUC）위0．763，고우전통유선종류표지물CEA화CA153。유선암환자혈장miR－127－3p적표체수평재불동종류직경、분화정도화TNM（ tumor node metastasis ）분기간차이무통계학의의（ P＞0．05）。결론 miR－127－3p재유선암환자중고표체，가능시진단유선암환자적일개중요삼고지표。（중화검험의학잡지，2015，38：682－685）
Objective To investigate the expression and clinical value of miR-127-3p in plasma of patients with breast cancer .Methods 80 cases of breast patients , 70 cases of benign breast tumor patients and 70 cases of normal control group were recruited .A real-time fluorescent quantitative reverse transcription polymerase chain reaction ( RT-PCR ) method for detecting miR-127-3p was established; Liner, reproducibility, and specificity were evaluated.In addition, correlations between the relative expression of plasma miR-127-3p and the concentrations of CEA and CA 153 were assessed.the relationship of miR-127-3p expression and clinicopathological features was further determined by Mann-Whitney test.Results The method for detection of plasma miR-127-3p was established.The relative plasma expression of miR-127-3p in breast patients [ 10.561 ( 5.424 -16.465 ) ] was significantly higher than that in benign breast tumor patients [3.015 (1.987-5.035)] (P=0.000 6) and healthy controls [2.375 (1.173-4.370)] (P=0.000 2).However, there was no significant difference between benign tumor patients and healthy control group (P=0.143).Positive relationship was found between the relative expression of miR-127-3p and the concentration of CA153 (R2 =0.457, P=0.003).The area under the ROC curve (AUC) of miR-127-3pwas 0.763, which was higher than that of CEA and CA 153.No significant difference was found between plasma miR-127-3p expression and clinicalpathological features including tumor size , differentiation and tumor node metastasis stage (P>0.05).Conclusions miR-127-3p was increased in breast cancer patients and may be an important diagnostic index for breast cancer .