CAJ | 학술논문

目的：探讨COX-2抑制剂塞来昔布对人胰腺癌细胞系PANC-1增殖、侵袭、迁移能力的影响，确定塞来昔布作用的最佳浓度和最适宜的应用时间。方法不同浓度的塞来昔布（20、60和100μmol／L）处理胰腺癌细胞不同时间（24、48和72 h）后，用MTT比色法检测细胞的增殖能力；用Transwell实验检测细胞的侵袭能力；采用细胞划痕实验检测细胞的迁移能力。结果 MTT结果显示塞来昔布作用后胰腺癌细胞的增殖能力下降，呈时间和浓度依赖性（ P＜0.05）；Transwell侵袭实验结果显示塞来昔布作用后胰腺癌细胞的侵袭能力下降，呈浓度依赖性（ P＜0.01）；划痕实验结果显示塞来昔布作用后胰腺癌细胞的迁移能力下降，呈浓度依赖性（ P＜0.01）。结论塞来昔布以浓度梯度、时间梯度的形式减弱人胰腺癌细胞系PANC-1的增殖能力，以浓度梯度的形式减弱人胰腺癌细胞系PANC-1的侵袭及迁移能力。
목적：탐토COX-2억제제새래석포대인이선암세포계PANC-1증식、침습、천이능력적영향，학정새래석포작용적최가농도화최괄의적응용시간。방법불동농도적새래석포（20、60화100μmol／L）처리이선암세포불동시간（24、48화72 h）후，용MTT비색법검측세포적증식능력；용Transwell실험검측세포적침습능력；채용세포화흔실험검측세포적천이능력。결과 MTT결과현시새래석포작용후이선암세포적증식능력하강，정시간화농도의뢰성（ P＜0.05）；Transwell침습실험결과현시새래석포작용후이선암세포적침습능력하강，정농도의뢰성（ P＜0.01）；화흔실험결과현시새래석포작용후이선암세포적천이능력하강，정농도의뢰성（ P＜0.01）。결론새래석포이농도제도、시간제도적형식감약인이선암세포계PANC-1적증식능력，이농도제도적형식감약인이선암세포계PANC-1적침습급천이능력。
Objective To investigate the effects of cyclooxygenase-2 inhibitor celecoxib on proliferation , invasion and migration of human pancreatic cancer cell line PANC-1 and then determine the optimal concentration of cele-coxib and the most suitable application time .Methods Human pancreatic cancer cell line PANC-1 was treated with diverse concentrations of celecoxib (20,60,100 μmol/L) for different durations (24,48,72 h).Cell prolifer-ation, invasion and migration capabilities were measured by MTT colorimetry , Transwell invasion assay , and scratch assay respectively .Results The proliferation capability of PANC-1 cell was reduced by celecoxib in a con-centration-and time-dependent manner ( P <0.05 ) .In addition , the invasion and migration capabilities were decreased by celecoxib in a concentration-dependent manner(P<0.01).Conclusions Celecoxib attenuates the proliferation of human pancreatic cancer cell line PANC-1 in a concentration-and time-dependent manner .Cele-coxib attenuates the invasion and migration in a concentration-dependent manner .