中华妇产科杂志
中華婦產科雜誌
중화부산과잡지
Chinese Journal of Obstetrics and Gynecology
2015年
10期
747-751
,共5页
先兆子痫%内皮祖细胞%T淋巴细胞亚群%流式细胞术
先兆子癇%內皮祖細胞%T淋巴細胞亞群%流式細胞術
선조자간%내피조세포%T림파세포아군%류식세포술
Pre-eclampsia%Endothelial progenitor cells%T-lymphocyte subsets%Flow cytometry
目的 探讨血管生成性T细胞(Tang)及内皮祖细胞(EPC)与子痫前期发病的关系及两种细胞的相关性.方法 选取2013年3月至2014年8月在湖南省人民医院诊断为子痫前期的孕妇40例,其中轻度20例为子痫前期轻度组,重度20例为子痫前期重度组,另选取同期24例孕晚期健康孕妇作为健康孕妇组.采用流式细胞仪检测孕妇外周血浆中Tang和EPC的含量,对各组孕妇的临床指标和血浆中EPC和Tang的含量进行比较,并对Tang含量与EPC含量的相关性进行分析.结果(1)3组孕妇的年龄、孕前体质指数(BMI)、检测孕周分别比较,差异均无统计学意义(P>0.05).3组孕妇血压比较,差异均有统计学意义(P<0.05).子痫前期重度组孕妇分娩孕周、新生儿出生体质量和出生1分钟Apgar评分均明显低于子痫前期轻度组和健康孕妇组,分别比较,差异均有统计学意义(P<0.05).子痫前期重度组发生新生儿窒息7例(35%,7/20),子痫前期轻度组1例(5%,1/20),两组比较,差异有统计学意义(P<0.05).(2)健康孕妇组孕妇外周血浆中Tang含量为(52.7±8.0)%,子痫前期轻度组为(47.5±8.8)%,子痫前期重度组为(45.5±8.7)%,3组比较,差异有统计学意义(F=4.248,P<0.05);两两比较显示,子痫前期重度组低于健康孕妇组,差异有统计学意义(P<0.05),子痫前期轻度组与健康孕妇组及子痫前期重度组分别比较,差异无统计学意义(P>0.05).(3)健康孕妇组孕妇外周血浆中EPC含量为(0.16 ± 0.07)%,子痫前期轻度组为(0.09 ± 0.07)%,子痫前期重度组为(0.08 ± 0.05)%,3组比较,差异有统计学意义(F=9.351,P<0.05);子痫前期重度组和子痫前期轻度组均低于健康孕妇组,两者比较,差异有统计学意义(P<0.05).(4)健康孕妇组孕妇外周血浆中Tang含量与EPC含量无显著相关性(r=-0.325,P>0.05);子痫前期重度组和轻度组孕妇外周血浆中Tang含量与EPC含量有显著相关性(r=0.677,P<0.01).子痫前期轻度组外周血浆中Tang含量与EPC含量有显著相关性(r=0. 803,P<0.01);子痫前期重度组外周血浆中Tang含量与EPC含量有显著相关性(r=0.520,P<0.05).结论 孕妇外周血中Tang含量下降可能与子痫前期的发病有关;在子痫前期孕妇外周血中Tang含量与EPC含量呈正相关,Tang对EPC含量变化有明显的影响作用,两者共同参与子痫前期孕妇的血管内皮损伤的修复.
目的 探討血管生成性T細胞(Tang)及內皮祖細胞(EPC)與子癇前期髮病的關繫及兩種細胞的相關性.方法 選取2013年3月至2014年8月在湖南省人民醫院診斷為子癇前期的孕婦40例,其中輕度20例為子癇前期輕度組,重度20例為子癇前期重度組,另選取同期24例孕晚期健康孕婦作為健康孕婦組.採用流式細胞儀檢測孕婦外週血漿中Tang和EPC的含量,對各組孕婦的臨床指標和血漿中EPC和Tang的含量進行比較,併對Tang含量與EPC含量的相關性進行分析.結果(1)3組孕婦的年齡、孕前體質指數(BMI)、檢測孕週分彆比較,差異均無統計學意義(P>0.05).3組孕婦血壓比較,差異均有統計學意義(P<0.05).子癇前期重度組孕婦分娩孕週、新生兒齣生體質量和齣生1分鐘Apgar評分均明顯低于子癇前期輕度組和健康孕婦組,分彆比較,差異均有統計學意義(P<0.05).子癇前期重度組髮生新生兒窒息7例(35%,7/20),子癇前期輕度組1例(5%,1/20),兩組比較,差異有統計學意義(P<0.05).(2)健康孕婦組孕婦外週血漿中Tang含量為(52.7±8.0)%,子癇前期輕度組為(47.5±8.8)%,子癇前期重度組為(45.5±8.7)%,3組比較,差異有統計學意義(F=4.248,P<0.05);兩兩比較顯示,子癇前期重度組低于健康孕婦組,差異有統計學意義(P<0.05),子癇前期輕度組與健康孕婦組及子癇前期重度組分彆比較,差異無統計學意義(P>0.05).(3)健康孕婦組孕婦外週血漿中EPC含量為(0.16 ± 0.07)%,子癇前期輕度組為(0.09 ± 0.07)%,子癇前期重度組為(0.08 ± 0.05)%,3組比較,差異有統計學意義(F=9.351,P<0.05);子癇前期重度組和子癇前期輕度組均低于健康孕婦組,兩者比較,差異有統計學意義(P<0.05).(4)健康孕婦組孕婦外週血漿中Tang含量與EPC含量無顯著相關性(r=-0.325,P>0.05);子癇前期重度組和輕度組孕婦外週血漿中Tang含量與EPC含量有顯著相關性(r=0.677,P<0.01).子癇前期輕度組外週血漿中Tang含量與EPC含量有顯著相關性(r=0. 803,P<0.01);子癇前期重度組外週血漿中Tang含量與EPC含量有顯著相關性(r=0.520,P<0.05).結論 孕婦外週血中Tang含量下降可能與子癇前期的髮病有關;在子癇前期孕婦外週血中Tang含量與EPC含量呈正相關,Tang對EPC含量變化有明顯的影響作用,兩者共同參與子癇前期孕婦的血管內皮損傷的脩複.
목적 탐토혈관생성성T세포(Tang)급내피조세포(EPC)여자간전기발병적관계급량충세포적상관성.방법 선취2013년3월지2014년8월재호남성인민의원진단위자간전기적잉부40례,기중경도20례위자간전기경도조,중도20례위자간전기중도조,령선취동기24례잉만기건강잉부작위건강잉부조.채용류식세포의검측잉부외주혈장중Tang화EPC적함량,대각조잉부적림상지표화혈장중EPC화Tang적함량진행비교,병대Tang함량여EPC함량적상관성진행분석.결과(1)3조잉부적년령、잉전체질지수(BMI)、검측잉주분별비교,차이균무통계학의의(P>0.05).3조잉부혈압비교,차이균유통계학의의(P<0.05).자간전기중도조잉부분면잉주、신생인출생체질량화출생1분종Apgar평분균명현저우자간전기경도조화건강잉부조,분별비교,차이균유통계학의의(P<0.05).자간전기중도조발생신생인질식7례(35%,7/20),자간전기경도조1례(5%,1/20),량조비교,차이유통계학의의(P<0.05).(2)건강잉부조잉부외주혈장중Tang함량위(52.7±8.0)%,자간전기경도조위(47.5±8.8)%,자간전기중도조위(45.5±8.7)%,3조비교,차이유통계학의의(F=4.248,P<0.05);량량비교현시,자간전기중도조저우건강잉부조,차이유통계학의의(P<0.05),자간전기경도조여건강잉부조급자간전기중도조분별비교,차이무통계학의의(P>0.05).(3)건강잉부조잉부외주혈장중EPC함량위(0.16 ± 0.07)%,자간전기경도조위(0.09 ± 0.07)%,자간전기중도조위(0.08 ± 0.05)%,3조비교,차이유통계학의의(F=9.351,P<0.05);자간전기중도조화자간전기경도조균저우건강잉부조,량자비교,차이유통계학의의(P<0.05).(4)건강잉부조잉부외주혈장중Tang함량여EPC함량무현저상관성(r=-0.325,P>0.05);자간전기중도조화경도조잉부외주혈장중Tang함량여EPC함량유현저상관성(r=0.677,P<0.01).자간전기경도조외주혈장중Tang함량여EPC함량유현저상관성(r=0. 803,P<0.01);자간전기중도조외주혈장중Tang함량여EPC함량유현저상관성(r=0.520,P<0.05).결론 잉부외주혈중Tang함량하강가능여자간전기적발병유관;재자간전기잉부외주혈중Tang함량여EPC함량정정상관,Tang대EPC함량변화유명현적영향작용,량자공동삼여자간전기잉부적혈관내피손상적수복.
Objectives To investigate the role of angiogenic T cells (Tang) and endothelial progenitor cells (EPC) in the pathogenesis of preeclampsia. To explore the relationship between Tang and EPC. Methods From Mar 2013 to Aug 2014, 40 patients diagnosed preeclampsia (PE) and delivered in Hunan Provincial People′s Hospital. A total of 20 of them were defined as the mild preeclampsia group and the other 20 cases were recruited as the severe preeclampsia group. And 24 healthy pregnant women wererecruited as the control group. The percentage of Tang and EPC in peripheral blood mononuclear cells (PBMC) were determinated by flow cytometry between 28 and 40 gestational weeks. Results (1) There was no significant difference in the age, pre-pregnancy body mass index(Pre-BMI) or gestational age among the three groups (P>0.05). The differences of blood pressure among the three groups were statistically significant (P<0.05). The gestational week at delivery, the birthweight of the neonates and the 1 minute Apgar score in the severe preeclampsia group were lower than those in the mild preeclampsia group and the control group, with statistically significant differences (P<0.05). The morbidity of neonatal asphyxia in the severe preeclampsia group was 35%(7/20);and in the mild preeclampsia group it was 5%(1/20), with statistically significant difference( P<0.05). (2) The percentage of Tang in maternal peripheral blood was(52.7 ± 8.0)%, (47.5 ± 8.8)% and (45.5 ± 8.7)% in the control group, the mild preeclampsia group and the severe preeclampsia group, respectively. The difference among the three groups was significant (F=4.248,P<0.05), and SNK q analysis showed there was significant difference between the control group and the severe preeclampsia group(P<0.05).While there was no statistically significant difference between the mild and the severe preeclampsia group, nor between the control group and the mild preeclampsia group(P>0.05). (3) The percentage of EPC in maternal peripheral blood was (0.16±0.07)%, (0.09±0.07)%and (0.08±0.05)%in the control group, the mild and the severe preeclampsia group, respectively. Analysis of variance showed that difference among the three groups was significant (F=9.351, P<0.05). The percentage of EPC in the mild or the severe preeclampsia group was significantly higher than that of the control group(P<0.05). (4) There was no statistically significant correlation between the Tang level and the EPC level in the control group ( r=-0.325, P>0.05). In the preeclampsia group (including mild and severe cases), there was positive correlation between the Tang level and EPC level (r=0.667, P<0.01). The positive correlation between Tang level and EPC level were proved respectively in the mild preeclampsia group (r=0.803, P<0.01) and the severe preeclampsia group (r= 0.520, P<0.05). Conclusions The number of Tang had some correlation with the pathogenesis of preeclampsia. The percentage of Tang had positive correlation with the level of EPC in women with preeclampsia. Tang might have some influence on the change of EPC′ level. Tang together with EPC were likely to contribute to the angiogenesis in preeclampsia.
