医学研究生学报
醫學研究生學報
의학연구생학보
Journal of Medical Postgraduates
2015年
11期
1171-1174
,共4页
陈昕%郭志良%叶瑞东%姜永军%俞书红%肖露露%孙文%李华%朱武生%徐格林%刘新峰
陳昕%郭誌良%葉瑞東%薑永軍%俞書紅%肖露露%孫文%李華%硃武生%徐格林%劉新峰
진흔%곽지량%협서동%강영군%유서홍%초로로%손문%리화%주무생%서격림%류신봉
脑卒中%溶栓%出血转化%大鼠模型
腦卒中%溶栓%齣血轉化%大鼠模型
뇌졸중%용전%출혈전화%대서모형
Stroke%Thrombolysis%Hemorrhagic transformation%Rat model
目的：目前尚缺乏与临床接近的重组组织型纤溶酶原激活剂（ recombinant tissue-type plasminogen activator ， rtPA）溶栓后出血转化的动物模型。文中建立一种贴近临床的用于研究卒中溶栓后出血转化的大鼠脑血栓栓塞模型。方法文中通过对栓子注入部位及溶栓时间进行改进，将60只SD大鼠按随机数字表法分为假手术组、脑栓塞组与rtPA溶栓组。取大鼠股动脉血制备体外血栓，脑栓塞组与rtPA溶栓组将血栓注入颈内动脉建立脑血栓栓塞模型，假手术组注入含BSA的PBS。栓子注入5 h后，rtPA溶栓组静脉溶栓，脑栓塞组和假手术组则注入等渗盐水。栓子注入24 h后对大鼠进行神经功能评分，大鼠心脏灌流后处死，取脑进行TTC染色，评价梗死体积与脑水肿程度，并采用分光光度法检测出血体积。结果 rtPA溶栓组与脑栓塞组的梗死体积[（29．29±4．204）％ vs （27．89±3．91）％，P＝0．8103]、脑水肿程度[（12．43±1．66）％ vs （7．13±2．04）％，P＝0．0632]、神经功能评分[（3．35±0．27）分 vs （2．80±0．28）分，P＝0．174）]差异均无统计学意义，但出血体积溶栓组明显高于脑栓塞组[（17．55±2．20）μL vs （3．82±0．86）μL，P＜0．01]。结论溶栓后出血转化的大鼠脑血栓栓塞模型能形成与临床rtPA静脉溶栓发生出血转化的病理改变，稳定性和重复性较好，可用于rtPA溶栓出血转化发病机制的相关研究。
目的：目前尚缺乏與臨床接近的重組組織型纖溶酶原激活劑（ recombinant tissue-type plasminogen activator ， rtPA）溶栓後齣血轉化的動物模型。文中建立一種貼近臨床的用于研究卒中溶栓後齣血轉化的大鼠腦血栓栓塞模型。方法文中通過對栓子註入部位及溶栓時間進行改進，將60隻SD大鼠按隨機數字錶法分為假手術組、腦栓塞組與rtPA溶栓組。取大鼠股動脈血製備體外血栓，腦栓塞組與rtPA溶栓組將血栓註入頸內動脈建立腦血栓栓塞模型，假手術組註入含BSA的PBS。栓子註入5 h後，rtPA溶栓組靜脈溶栓，腦栓塞組和假手術組則註入等滲鹽水。栓子註入24 h後對大鼠進行神經功能評分，大鼠心髒灌流後處死，取腦進行TTC染色，評價梗死體積與腦水腫程度，併採用分光光度法檢測齣血體積。結果 rtPA溶栓組與腦栓塞組的梗死體積[（29．29±4．204）％ vs （27．89±3．91）％，P＝0．8103]、腦水腫程度[（12．43±1．66）％ vs （7．13±2．04）％，P＝0．0632]、神經功能評分[（3．35±0．27）分 vs （2．80±0．28）分，P＝0．174）]差異均無統計學意義，但齣血體積溶栓組明顯高于腦栓塞組[（17．55±2．20）μL vs （3．82±0．86）μL，P＜0．01]。結論溶栓後齣血轉化的大鼠腦血栓栓塞模型能形成與臨床rtPA靜脈溶栓髮生齣血轉化的病理改變，穩定性和重複性較好，可用于rtPA溶栓齣血轉化髮病機製的相關研究。
목적：목전상결핍여림상접근적중조조직형섬용매원격활제（ recombinant tissue-type plasminogen activator ， rtPA）용전후출혈전화적동물모형。문중건립일충첩근림상적용우연구졸중용전후출혈전화적대서뇌혈전전새모형。방법문중통과대전자주입부위급용전시간진행개진，장60지SD대서안수궤수자표법분위가수술조、뇌전새조여rtPA용전조。취대서고동맥혈제비체외혈전，뇌전새조여rtPA용전조장혈전주입경내동맥건립뇌혈전전새모형，가수술조주입함BSA적PBS。전자주입5 h후，rtPA용전조정맥용전，뇌전새조화가수술조칙주입등삼염수。전자주입24 h후대대서진행신경공능평분，대서심장관류후처사，취뇌진행TTC염색，평개경사체적여뇌수종정도，병채용분광광도법검측출혈체적。결과 rtPA용전조여뇌전새조적경사체적[（29．29±4．204）％ vs （27．89±3．91）％，P＝0．8103]、뇌수종정도[（12．43±1．66）％ vs （7．13±2．04）％，P＝0．0632]、신경공능평분[（3．35±0．27）분 vs （2．80±0．28）분，P＝0．174）]차이균무통계학의의，단출혈체적용전조명현고우뇌전새조[（17．55±2．20）μL vs （3．82±0．86）μL，P＜0．01]。결론용전후출혈전화적대서뇌혈전전새모형능형성여림상rtPA정맥용전발생출혈전화적병리개변，은정성화중복성교호，가용우rtPA용전출혈전화발병궤제적상관연구。
Objective The purpose of this study was to establish a rat model of thromboembolism for the study of hemorrhag -ic transformation after intravenous thrombolysis with the recombinant tissue plasminogen activator ( rtPA) . Methods Sixty Sprague-Dawley rats were randomly divided into a sham operation , a cerebral embolism, and an rtPA group.Thrombus was prepared in vitro with the rat femoral artery blood and injected into the internal carotid artery of the rats in the cerebral embolism and rtPA groups to es -tablish a model of embolic focal cerebral ischemia , while the animals of the sham operation group injected with BSA .Five hours later , the rats in the rtPA group received rtPA and those in the cerebral embol-ism and sham operation groups the injection of isotonic saline solu-tion.At 24 hours after embolus injection , the neurological deficit score was obtained .The rats were sacrificed after cardiac perfusion and their brains removed for triphenyltetrazolium chloride staining , assessment of the infarct volume and cerebral edema , and calculation of the hemorrhage volume by spectrophotometric hemoglobin assay . Results The hemorrhage volume was significantly higher in the rtPA than in the cerebral embolism group ([17.55 ±2.20] μL vs [3.82 ±0.86] μL, P<0.01), but there were no statistically significant differences between the two groups in the infarct volume ([29.29 ±4.204] %vs [27.89 ±3.91] %, P=0.810), cerebral edema ([12.43 ±1.66] % vs [7.13 ±2.04] %,P=0.063 2), and neurological deficit score (3.35 ±0.27 vs 2.80 ±0.28, P=0.174). Conclusion The rat model of thromboembolism, with a high stability and reproducibility , can be used for the pathogenesis-related studies of hemorrhagic transformation after thromboly-sis with rtPA.
