CAJ | 학술논문

目的：探讨miRNA-449a在人肺癌及癌旁组织中的表达差异及其临床意义。方法右江民族医学院附属医院2011-01-01—2013-06-30收治的肺癌患者58例，对照组为癌旁正常组织，病例组为鳞癌组织和腺癌组织，并根据miRNA-449 a序列结构设计合成miRNA-449 a模拟物，设10和20 mg／mL两个不同浓度，以0 mg／mL为阴性对照，用real-time PCR检测肺癌及其对应癌旁组织中miRNA-449 a的表达；化学发光技术检测细胞内荧光素酶基因表达；四甲基偶氮唑蓝法检测细胞活性。结果鳞癌组和腺癌组的miRNA-449a的表达量为1.48±1.63和1.52±1.54，均显著低于癌旁正常对照组的2.74±1.55（ P＜0.01），且与瘤体大小有关（ P＜0.05）。10和20 mg／mL 组细胞内的平均荧光强度为2115±168和1352±159，均显著低于阴性对照组的4975±115（ P＜0.01），且抑制作用随浓度增加呈递增趋势。结论 miRNA-449 a在肺癌组织中呈低表达，可下调细胞内荧光蛋白表达和诱导细胞凋亡。
목적：탐토miRNA-449a재인폐암급암방조직중적표체차이급기림상의의。방법우강민족의학원부속의원2011-01-01—2013-06-30수치적폐암환자58례，대조조위암방정상조직，병례조위린암조직화선암조직，병근거miRNA-449 a서렬결구설계합성miRNA-449 a모의물，설10화20 mg／mL량개불동농도，이0 mg／mL위음성대조，용real-time PCR검측폐암급기대응암방조직중miRNA-449 a적표체；화학발광기술검측세포내형광소매기인표체；사갑기우담서람법검측세포활성。결과린암조화선암조적miRNA-449a적표체량위1.48±1.63화1.52±1.54，균현저저우암방정상대조조적2.74±1.55（ P＜0.01），차여류체대소유관（ P＜0.05）。10화20 mg／mL 조세포내적평균형광강도위2115±168화1352±159，균현저저우음성대조조적4975±115（ P＜0.01），차억제작용수농도증가정체증추세。결론 miRNA-449 a재폐암조직중정저표체，가하조세포내형광단백표체화유도세포조망。
Objective To investigate the expression and biological function of miRNA-449 a in lung cancer . Methods A case-control study was conducted in 58 patients diagnosed with lung cancer ( carcinoma and adeno-carcinoma) and normal tissue closely adjacent to tumor.MiRNA-449a simulation was designed and synthesized, was dissolved into two different concentrations as 10 and 20 mg/mL.The expression of miRNA-449a in lung cancer tissues and matched normal tissues were detected by Real time PCR .The expression of luciferase gene was detected by chemiluminescence technique.MiRNA-449a mimics on cell apoptosis was evaluated by MTT assay . Results The mean tissues expression levels of miRNA-449 a in squamous carcinoma group and adenocarcinoma group were 1.48 ±1.63 and 1.52 ±1.54 respectively, and were significantly lower than in control group (2.74 ± 1.55 ) ( P<0.01 ) .The average intensity of fluorescent protein in 10 mg/mL group and 20 mg/mL group were 2 115 ±168 and 1 352 ±159 respectively , and were significantly lower than that in control group ( 4 975 ±115 ) ( P<0.01 ) .Conclusions MiRNA-449 a was down-regulated expression in lung cancer and induced apoptosis .