医学研究生学报
醫學研究生學報
의학연구생학보
Journal of Medical Postgraduates
2015年
11期
1138-1142
,共5页
张子新%吉虓%慕翠翠%余陆娇
張子新%吉虓%慕翠翠%餘陸嬌
장자신%길효%모취취%여륙교
高血糖%高血脂%Rho激酶%内皮功能
高血糖%高血脂%Rho激酶%內皮功能
고혈당%고혈지%Rho격매%내피공능
Hyperglycaemia%Hyperlipid%Rho kinase%Endothelial Function
目的:高血糖合并高血脂在临床心血管疾病中非常普遍,文中通过观察高血糖高血脂大鼠主动脉内皮功能和Rho激酶的表达,研究Rho激酶与内皮功能的关系及Rho激酶抑制剂对其的影响。方法健康雄性Wistar大鼠随机数字表法分成空白对照组(5只)、模型组(10只)、药物组(10只)。模型组和药物组先采用经尾静脉注射链脲佐菌素加高脂饮食制备高血糖高血脂的模型,再分别给予等渗盐水、Rho激酶抑制剂法舒地尔腹腔注射治疗4周。实验结束后测定3组大鼠血糖( VBG)、三酰甘油( TG)、血清总胆固醇( TC)、血清低密度脂蛋白胆固醇( LDL)、血清高密度脂蛋白胆固醇( HDL)、一氧化氮合酶(eNOS)、内皮素(ET-1)表达水平以及肌球蛋白磷酸酶结合亚基1(MYPT-1)和Rho激酶的mRNA表达。 HE染色观察内皮形态学变化。结果与空白对照组比较,模型组VBG、ET-1、MYPT-1、ROCK和RhoA mRNA水平明显升高[(6.18±1.14) mmol/L vs (26.36±3.25)mmol/L,(72.13±6.13)pg/mL vs (88.22±4.61)pg/mL,(0.54±0.08) vs (1.28±0.17),(0.16±0.20) vs (0.83±0.12),(0.40±0.18) vs (0.78±0.13),P <0.05],TG、TC、LDL 亦升高(P <0.05),eNOS 水平降低[(24.42±2.13)U/L vs (17.36±1.58) U/L,P <0.05]。与模型组比较,药物组 VBG[(17.70±2.69) mmol/L]、 ET-1[(75.03±2.50)pg/mL]、MYPT-1(0.74±0.11)、ROCK mRNA水平(0.40±0.08)和RhoAmRNA(0.25±0.07)水平减低(P<0.05),TG[(1.56±0.14)mmol/L]、TC[(6.26±0.43)mmol/L]、LDL[(4.76±0.57)mmol/L]亦降低(P<0.05),eNOS水平[(22.83±1.60)U/L]明显升高(P<0.05)。内皮形态学变化:空白对照组大鼠主动脉内皮形态正常,模型组主动脉内皮病理损伤较重,药物组主动脉内皮损伤明显改善。结论高血糖高血脂大鼠存在内皮功能障碍和 Rho激酶高表达,Rho激酶抑制剂可改善其内皮功能。
目的:高血糖閤併高血脂在臨床心血管疾病中非常普遍,文中通過觀察高血糖高血脂大鼠主動脈內皮功能和Rho激酶的錶達,研究Rho激酶與內皮功能的關繫及Rho激酶抑製劑對其的影響。方法健康雄性Wistar大鼠隨機數字錶法分成空白對照組(5隻)、模型組(10隻)、藥物組(10隻)。模型組和藥物組先採用經尾靜脈註射鏈脲佐菌素加高脂飲食製備高血糖高血脂的模型,再分彆給予等滲鹽水、Rho激酶抑製劑法舒地爾腹腔註射治療4週。實驗結束後測定3組大鼠血糖( VBG)、三酰甘油( TG)、血清總膽固醇( TC)、血清低密度脂蛋白膽固醇( LDL)、血清高密度脂蛋白膽固醇( HDL)、一氧化氮閤酶(eNOS)、內皮素(ET-1)錶達水平以及肌毬蛋白燐痠酶結閤亞基1(MYPT-1)和Rho激酶的mRNA錶達。 HE染色觀察內皮形態學變化。結果與空白對照組比較,模型組VBG、ET-1、MYPT-1、ROCK和RhoA mRNA水平明顯升高[(6.18±1.14) mmol/L vs (26.36±3.25)mmol/L,(72.13±6.13)pg/mL vs (88.22±4.61)pg/mL,(0.54±0.08) vs (1.28±0.17),(0.16±0.20) vs (0.83±0.12),(0.40±0.18) vs (0.78±0.13),P <0.05],TG、TC、LDL 亦升高(P <0.05),eNOS 水平降低[(24.42±2.13)U/L vs (17.36±1.58) U/L,P <0.05]。與模型組比較,藥物組 VBG[(17.70±2.69) mmol/L]、 ET-1[(75.03±2.50)pg/mL]、MYPT-1(0.74±0.11)、ROCK mRNA水平(0.40±0.08)和RhoAmRNA(0.25±0.07)水平減低(P<0.05),TG[(1.56±0.14)mmol/L]、TC[(6.26±0.43)mmol/L]、LDL[(4.76±0.57)mmol/L]亦降低(P<0.05),eNOS水平[(22.83±1.60)U/L]明顯升高(P<0.05)。內皮形態學變化:空白對照組大鼠主動脈內皮形態正常,模型組主動脈內皮病理損傷較重,藥物組主動脈內皮損傷明顯改善。結論高血糖高血脂大鼠存在內皮功能障礙和 Rho激酶高錶達,Rho激酶抑製劑可改善其內皮功能。
목적:고혈당합병고혈지재림상심혈관질병중비상보편,문중통과관찰고혈당고혈지대서주동맥내피공능화Rho격매적표체,연구Rho격매여내피공능적관계급Rho격매억제제대기적영향。방법건강웅성Wistar대서수궤수자표법분성공백대조조(5지)、모형조(10지)、약물조(10지)。모형조화약물조선채용경미정맥주사련뇨좌균소가고지음식제비고혈당고혈지적모형,재분별급여등삼염수、Rho격매억제제법서지이복강주사치료4주。실험결속후측정3조대서혈당( VBG)、삼선감유( TG)、혈청총담고순( TC)、혈청저밀도지단백담고순( LDL)、혈청고밀도지단백담고순( HDL)、일양화담합매(eNOS)、내피소(ET-1)표체수평이급기구단백린산매결합아기1(MYPT-1)화Rho격매적mRNA표체。 HE염색관찰내피형태학변화。결과여공백대조조비교,모형조VBG、ET-1、MYPT-1、ROCK화RhoA mRNA수평명현승고[(6.18±1.14) mmol/L vs (26.36±3.25)mmol/L,(72.13±6.13)pg/mL vs (88.22±4.61)pg/mL,(0.54±0.08) vs (1.28±0.17),(0.16±0.20) vs (0.83±0.12),(0.40±0.18) vs (0.78±0.13),P <0.05],TG、TC、LDL 역승고(P <0.05),eNOS 수평강저[(24.42±2.13)U/L vs (17.36±1.58) U/L,P <0.05]。여모형조비교,약물조 VBG[(17.70±2.69) mmol/L]、 ET-1[(75.03±2.50)pg/mL]、MYPT-1(0.74±0.11)、ROCK mRNA수평(0.40±0.08)화RhoAmRNA(0.25±0.07)수평감저(P<0.05),TG[(1.56±0.14)mmol/L]、TC[(6.26±0.43)mmol/L]、LDL[(4.76±0.57)mmol/L]역강저(P<0.05),eNOS수평[(22.83±1.60)U/L]명현승고(P<0.05)。내피형태학변화:공백대조조대서주동맥내피형태정상,모형조주동맥내피병리손상교중,약물조주동맥내피손상명현개선。결론고혈당고혈지대서존재내피공능장애화 Rho격매고표체,Rho격매억제제가개선기내피공능。
Objective Hyperglycaemia and hyperlipid are common in clinical cardiovascular disease .The article was to ob-serve the change of endothelial and the expression of Rho-kinase in the aorta of rats with hyperglycaemia and hyperlipid and the rela-tionship between them .Methods Healthy male Wistar rats were randomly divided into 3 groups:5 as normal control group , 10 as model group and 10 as drug intervention group .The latter two groups were given high-fat diets for 5 weeks after tail vein injection of streptozocin (STZ) to establish hyperglycaemia and hyperlipid models , then fasudil (inhibitor of Rho-kinases) or saline were respec-tively given intraperitoneally for 4 weeks.Venous blood glucose (VBG), blood lipid levels, ET-1 and eNOS were tested, along with the expression of MYPT-1(the protein substrates of ROCK)、mRNA of RhoA and ROCK. Results Compared with normal control group, the levels of VBG、ET-1、MYPT-1、mRNA of ROCK and Rho A were increased in model group[(6.18±1.14)mmol/L vs (26.36± 3.25)mmol/L,(72.13±6.13 )pg/mL vs (88.22±4.61) pg/mL, (0.54±0.08) v s (1.28±0.17),(0.16±0.20) vs (0.83± 0.12),(0.40±0.18) vs (0.78±0.13),P<0.05].TG、TC、LDL were also higher in model group(P<0.05), while eNOS level was lower in model group than in control group[(24.42±2.13)U/L sv (17.36±1.58)U/L,P<0.05].Compared with model group, the levels of VBG[(17.70±2.69)mmol/L]、TG、TC、LDL, ET-1 [(75.03±2.50)pg/mL]、MYPT-1(0.74±0.01)、mRNA of ROCK(04.0±0.08) and Rho A (0.25±0.07)in drug intervention group were lower(P<0.05), while eNOS level (0.74±0.11) was higher(P<0.05) in drug intervention group than in model group . Conclusion Endothelial dysfunction and over-expression of Rho kinase can be found in hyperglycaemia and hyperlipid rat models .Fa-sudil could improve endothelial function .