医学研究生学报
醫學研究生學報
의학연구생학보
Journal of Medical Postgraduates
2015年
11期
1124-1127
,共4页
非酒精性脂肪肝%小肠黏膜屏障%闭锁小带蛋白%连接蛋白
非酒精性脂肪肝%小腸黏膜屏障%閉鎖小帶蛋白%連接蛋白
비주정성지방간%소장점막병장%폐쇄소대단백%련접단백
Non alchohol fatty liver disease%Intestinal barrier function%Zonula occludens-1%Occluding
目的：肠道黏膜屏障功能紊乱与非酒精性脂肪肝（ non-alcohol fatty liver disease ，NAFLD）的发生关系密切，文中旨在通过动物模型的方法探讨NAFLD大鼠肠道黏膜屏障功能。方法 SD大鼠16只按随机数字表法分为正常组和NAFLD组，每组8只。正常组由普通饲料喂养，每100克普通饲料提供326 kCal热量。 NAFLD组大鼠由高脂饲料喂养，每100克高脂饲料提供453 kCal热量建立NAFLD模型。采用HE染色检测肝病理， Elisa法和鲎试剂法分别检测肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）、白细胞介素-1（Interleukin-1，IL-1）、内毒素，实时定量PCR法检测小肠闭锁小带蛋白ZO-1和连接蛋白Occluding的表达。结果 NAFLD组大鼠肝呈现典型的脂质沉积，血浆内毒素和血清TNF-α、IL-1较正常组显著增高，小肠ZO-1和Occluding表达较正常组显著降低（P＜0．05）。结论 NAFLD大鼠存在肠道黏膜屏障功能下降。
目的：腸道黏膜屏障功能紊亂與非酒精性脂肪肝（ non-alcohol fatty liver disease ，NAFLD）的髮生關繫密切，文中旨在通過動物模型的方法探討NAFLD大鼠腸道黏膜屏障功能。方法 SD大鼠16隻按隨機數字錶法分為正常組和NAFLD組，每組8隻。正常組由普通飼料餵養，每100剋普通飼料提供326 kCal熱量。 NAFLD組大鼠由高脂飼料餵養，每100剋高脂飼料提供453 kCal熱量建立NAFLD模型。採用HE染色檢測肝病理， Elisa法和鱟試劑法分彆檢測腫瘤壞死因子-α（tumor necrosis factor-α，TNF-α）、白細胞介素-1（Interleukin-1，IL-1）、內毒素，實時定量PCR法檢測小腸閉鎖小帶蛋白ZO-1和連接蛋白Occluding的錶達。結果 NAFLD組大鼠肝呈現典型的脂質沉積，血漿內毒素和血清TNF-α、IL-1較正常組顯著增高，小腸ZO-1和Occluding錶達較正常組顯著降低（P＜0．05）。結論 NAFLD大鼠存在腸道黏膜屏障功能下降。
목적：장도점막병장공능문란여비주정성지방간（ non-alcohol fatty liver disease ，NAFLD）적발생관계밀절，문중지재통과동물모형적방법탐토NAFLD대서장도점막병장공능。방법 SD대서16지안수궤수자표법분위정상조화NAFLD조，매조8지。정상조유보통사료위양，매100극보통사료제공326 kCal열량。 NAFLD조대서유고지사료위양，매100극고지사료제공453 kCal열량건립NAFLD모형。채용HE염색검측간병리， Elisa법화후시제법분별검측종류배사인자-α（tumor necrosis factor-α，TNF-α）、백세포개소-1（Interleukin-1，IL-1）、내독소，실시정량PCR법검측소장폐쇄소대단백ZO-1화련접단백Occluding적표체。결과 NAFLD조대서간정현전형적지질침적，혈장내독소화혈청TNF-α、IL-1교정상조현저증고，소장ZO-1화Occluding표체교정상조현저강저（P＜0．05）。결론 NAFLD대서존재장도점막병장공능하강。
Objective Disorder of intestinal barrier function is associated with the non-alcohol fatty liver disease ( NAFLD) . The present paper aimed to explore the intestinal barrier function in the rat model of NAFLD . Methods Sixteen SD rats were ran-domly divided into an NAFLD and a normal control group of equal number .The NAFLD models were constructed by high-fat feeding . HE staining was used for pathologic examination of the liver , the levels of TNF-α, IL-1, and endotoxin (ET ) were measured by ELISA and the limulus reagent method , and the expressions of intestinal ZO-1 and Occluding were determined by real time PCR . Resu lts Compared with the normal controls , the NAFLD rats showed typical hepatic lipid deposition , with significantly increased levels of serum TNF-αand IL-1 and plasma ET and decreased expressions of intestinal ZO-1 and Occluding (P<0.05). Conclusion Intestinal barrier function is decreased in NAFLD rats .
