基础医学与临床
基礎醫學與臨床
기출의학여림상
Basic & Clinical Medicine
2015年
1期
33-37
,共5页
子痫前期%炎性反应%高迁移率组蛋白%TOLL受体4%核转录因子
子癇前期%炎性反應%高遷移率組蛋白%TOLL受體4%覈轉錄因子
자간전기%염성반응%고천이솔조단백%TOLL수체4%핵전록인자
preeclampsia%inflammatory reaction%HMGB1%TLR4%NF-κB P65
目的：探讨高迁移率族蛋白（HMGB1）、TOLL受体4（TLR4）和NF-B信号通路在子痫前期中的相关作用。方法轻度子痫前期患者10例、重度子痫前期患者20例和同期正常妊娠者30例。免疫组织化学（ SP法）检测胎盘中HMGB1、TLR4和NF-κB P65蛋白的表达变化及在组织中的定性、定位；用ELISA法检测血清中HMGB1、TLR4和NF-κB P65蛋白浓度。结果子痫前期患者胎盘中HMGB1、TLR4、和NF-κB P65蛋白表达高于正常对照组（ P＜0.05）；轻度和重度子痫前期患者间无差异。子痫前期患者血清中HMGB1、TLR4和NF-κB P65的含量较正常组明显升高（P＜0.05）；且重度子痫前期患者血清中HMGB1、TLR4、和NF-κB P65蛋白表达高于轻度子痫前期患者（P＜0.05）。结论 HMGB1、TLR4及NF-κB P65蛋白表达水平在子痫前期患者胎盘及血清中显著升高，可能参与了子痫的发病过程。
目的：探討高遷移率族蛋白（HMGB1）、TOLL受體4（TLR4）和NF-B信號通路在子癇前期中的相關作用。方法輕度子癇前期患者10例、重度子癇前期患者20例和同期正常妊娠者30例。免疫組織化學（ SP法）檢測胎盤中HMGB1、TLR4和NF-κB P65蛋白的錶達變化及在組織中的定性、定位；用ELISA法檢測血清中HMGB1、TLR4和NF-κB P65蛋白濃度。結果子癇前期患者胎盤中HMGB1、TLR4、和NF-κB P65蛋白錶達高于正常對照組（ P＜0.05）；輕度和重度子癇前期患者間無差異。子癇前期患者血清中HMGB1、TLR4和NF-κB P65的含量較正常組明顯升高（P＜0.05）；且重度子癇前期患者血清中HMGB1、TLR4、和NF-κB P65蛋白錶達高于輕度子癇前期患者（P＜0.05）。結論 HMGB1、TLR4及NF-κB P65蛋白錶達水平在子癇前期患者胎盤及血清中顯著升高，可能參與瞭子癇的髮病過程。
목적：탐토고천이솔족단백（HMGB1）、TOLL수체4（TLR4）화NF-B신호통로재자간전기중적상관작용。방법경도자간전기환자10례、중도자간전기환자20례화동기정상임신자30례。면역조직화학（ SP법）검측태반중HMGB1、TLR4화NF-κB P65단백적표체변화급재조직중적정성、정위；용ELISA법검측혈청중HMGB1、TLR4화NF-κB P65단백농도。결과자간전기환자태반중HMGB1、TLR4、화NF-κB P65단백표체고우정상대조조（ P＜0.05）；경도화중도자간전기환자간무차이。자간전기환자혈청중HMGB1、TLR4화NF-κB P65적함량교정상조명현승고（P＜0.05）；차중도자간전기환자혈청중HMGB1、TLR4、화NF-κB P65단백표체고우경도자간전기환자（P＜0.05）。결론 HMGB1、TLR4급NF-κB P65단백표체수평재자간전기환자태반급혈청중현저승고，가능삼여료자간적발병과정。
Objective To evaluate the expression and discussion on high mobility group protein ( HMGB1 )/toll like receptor 4 ( TLR4 ) and NF-κB possible role in the signaling pathway in preeclampsia .Methods Ten patients with mild preeclampsia(MP), 20 patients with severe preeclampsia (SP) and 30 cases of normal pregnancy were recruited the same period.To check the expression of the HMGB1,TLR4,NF-κB P65 protein in placenta tissue using Immunohistochemical staining .Levels of HMGB1, TLR4, NF-κB P65 in blood serum were measured by ELISA.Results 1)ExpressionofHMGB1,TLR4,NF-κBP65wereincreasedascomparetocontrolgroup(P<0.05);HMGB1,TLR4,NF-κB P65 in placenta of patients with severe preeclampsia and mild preeclampsia failed to show significant difference .2) HMGB1,TLR4,NF-κB P65 in women with preeclampsia was significantly higher than control group ( P<0.05 ); HMGB1,TLR4,NF-κB P65 in women with severe preeclampsia showed a higher level as compared to mild preeclampsia (P<0.05).Conclusions HMGB1,TLR4 and NF-κB P65 were over-ex-pressed in the placenta tissue and serum in patients with preeclampsia , which indicated that HMGB1,TLR4 and NF-κB P65 may play an important role in the development of preeclampsia .
