CAJ | 학술논문

目的探讨高压氧预处理(HBOP)对急性高海拔暴露体力负荷人体应激反应的作用及可能机制.方法对8名男性受试者进行3、5和7d的HBOP,然后急性暴露于模拟4000m高海拔的低氧环境.安静状态分为3组:海平对照组、模拟高海拔组和HBOP干预5d组;负荷后分为4组:海平对照组、模拟高海拔组、HBOP干预3d组和7d组.在HBOP前后作自身对比体力负荷试验,并于运动前后检测血浆多巴胺(DA)、肾上腺素(E)、去甲肾上腺素(NE)和促肾上腺皮质激素(ACTH)水平.体力负荷方式:踏阶运动25次/min,5 min,阶梯高30 cm,用节拍器控制踏阶速度和计时,属中等体力劳动.结果急性高海拔暴露后机体在安静和体力负荷后的血浆DA、E、NE和ACTH水平均较海平对照组明显升高,差异均有统计学意义(均P＜0.05);HBOP干预5d组再次急性高海拔暴露后人体安静时血浆DA、E、NE和ACTH水平均较模拟高海拔组明显升高,差异均有统计学意义(均P＜0.01);HBOP干预3、7d组再次急性高海拔暴露体力负荷后的血浆DA、E、NE和ACTH水平(除HBOP干预3d组的ACTH)均较模拟高海拔组明显升高,差异均有统计学意义(均P＜0.01).结论 HBOP可以提高机体血浆DA、E、NE和ACTH表达水平,从而加快机体内环境稳态建立新的平衡以适应急性高原低氧环境.
목적 탐토고압양예처리(HBOP)대급성고해발폭로체력부하인체응격반응적작용급가능궤제.방법 대8명남성수시자진행3、5화7d적HBOP,연후급성폭로우모의4000m고해발적저양배경.안정상태분위3조:해평대조조、모의고해발조화HBOP간예5d조;부하후분위4조:해평대조조、모의고해발조、HBOP간예3d조화7d조.재HBOP전후작자신대비체력부하시험,병우운동전후검측혈장다파알(DA)、신상선소(E)、거갑신상선소(NE)화촉신상선피질격소(ACTH)수평.체력부하방식:답계운동25차/min,5 min,계제고30 cm,용절박기공제답계속도화계시,속중등체력노동.결과 급성고해발폭로후궤체재안정화체력부하후적혈장DA、E、NE화ACTH수평균교해평대조조명현승고,차이균유통계학의의(균P＜0.05);HBOP간예5d조재차급성고해발폭로후인체안정시혈장DA、E、NE화ACTH수평균교모의고해발조명현승고,차이균유통계학의의(균P＜0.01);HBOP간예3、7d조재차급성고해발폭로체력부하후적혈장DA、E、NE화ACTH수평(제HBOP간예3d조적ACTH)균교모의고해발조명현승고,차이균유통계학의의(균P＜0.01).결론 HBOP가이제고궤체혈장DA、E、NE화ACTH표체수평,종이가쾌궤체내배경은태건립신적평형이괄응급성고원저양배경.
Objective To explore the effects of hyperbaric oxygen preconditioning (HBOP) on human stress responses during acute exposure to high altitude and the possible mechanism.Methods Eight male subjects were treated with HBOP for 3,5,and 7 days,followed by acute exposure to hypoxia simulating an altitude of 4,000 m.Subjects at rest were divided into sea-level control group,simulated high-altitude group,and 5-day HBOP intervention group,while subjects after physical load were divided into sea-level control group,simulated high-altitude group,3-day HBOP intervention group,and 7-day HBOP intervention group.The physical load test was performed for each subject before and after HBOP,and the plasma levels of dopamine (DA),epinephrine (E),norepinephrine (NE),and adrenocorticotropic hormone (ACTH) were determined before and after exercise.The physical load test was performed by stepping up on to a 30 cm-high stepping stool at a rate of 25/min for 5 minutes,which was a type of moderate physical exercise.The stepping rate and timing were controlled by a metronome.Results The levels of DA,E,NE,and ACTH at rest and after physical load were significantly higher in subjects acutely exposed to high altitude than in the sea-level control groups (all P＜0.05).Moreover,the levels of DA,E,NE,and ACTH at rest were significantly higher after acute exposure to high altitude in the 5-day HBOP intervention group than in the simulated high-altitude group (all P＜0.01).Except for the ACTH level in the 3-day HBOP intervention group,the levels of DA,E,NE,and ACTH after physical load were significantly higher after acute exposure to high altitude in the 3-day and 7-day HBOP intervention groups than in the simulated high-altitude group (all P＜0.01).Conclusion HBOP can elevate the plasma expression of DA,E,NE,and ACTH,and then speed up the establishment of a new balance of homeostasis to adapt to the acute hypoxia at high altitude.