华中科技大学学报（医学版）
華中科技大學學報（醫學版）
화중과기대학학보（의학판）
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
2015年
5期
499-504
,共6页
许琮%夏超%李德民%常莹%赵秋
許琮%夏超%李德民%常瑩%趙鞦
허종%하초%리덕민%상형%조추
胰腺癌%姜黄素%吉西他滨%联合用药
胰腺癌%薑黃素%吉西他濱%聯閤用藥
이선암%강황소%길서타빈%연합용약
pancreatic cancer%curcumin%gemcitabine%combination therapy
目的：研究联合使用姜黄素及吉西他滨对胰腺癌细胞增殖、凋亡及肿瘤干细胞表型特征的影响，初步评价姜黄素治疗胰腺癌的临床应用前景。方法采用恶性程度、分化程度不同的胰腺癌细胞系panc03.27、Capan‐2及PANC‐1细胞，分为DM SO处理组、单纯吉西他滨处理组、单纯姜黄素处理组以及吉西他滨联合姜黄素处理组，采用CCK8（Cell Counting Kit‐8）试剂盒测定细胞增殖力的改变；同时采用FITC Annexin V凋亡检测试剂盒分析不同处理组之间细胞凋亡率的差异，利用Western blot检测胰腺癌干性基因Notch1及Oct4的表达改变。结果 Panc03.27细胞中与单纯使用吉西他滨比较，联合使用姜黄素组细胞活力由（46.70±3.49）％下降为（35.53±1.84）％（ P＝0.047），细胞凋亡率由（5.01±0.75）％上升至（13.11±0.89）％（P＜0.01），Notch1及Oct4表达明显下调（均 P＜0.01）；Capan‐2细胞中与单纯使用吉西他滨比较，联合使用姜黄素组细胞活力由（47.13±2.39）％下降至（5.36±0.25）％（ P＜0.01），细胞凋亡率由（13.02±1.79）％上升至（20.11±1.11）％（P＜0.01），而Notch1及Oct4表达未见明显差异；PANC‐1细胞中与单纯使用吉西他滨比联合使用姜黄素组细胞活力由（82.59±5.69）％下降至（7.33±0.25）％（ P＜0.01），细胞凋亡率由（6.77±2.03）％上升至（25.08±3.14）％（P＜0.01），Notch1及Oct4表达明显下调（P＜0.05）。结论姜黄素可协同吉西他滨抑制胰腺癌细胞的增殖、诱导细胞的凋亡及抑制肿瘤干细胞表型特征。
目的：研究聯閤使用薑黃素及吉西他濱對胰腺癌細胞增殖、凋亡及腫瘤榦細胞錶型特徵的影響，初步評價薑黃素治療胰腺癌的臨床應用前景。方法採用噁性程度、分化程度不同的胰腺癌細胞繫panc03.27、Capan‐2及PANC‐1細胞，分為DM SO處理組、單純吉西他濱處理組、單純薑黃素處理組以及吉西他濱聯閤薑黃素處理組，採用CCK8（Cell Counting Kit‐8）試劑盒測定細胞增殖力的改變；同時採用FITC Annexin V凋亡檢測試劑盒分析不同處理組之間細胞凋亡率的差異，利用Western blot檢測胰腺癌榦性基因Notch1及Oct4的錶達改變。結果 Panc03.27細胞中與單純使用吉西他濱比較，聯閤使用薑黃素組細胞活力由（46.70±3.49）％下降為（35.53±1.84）％（ P＝0.047），細胞凋亡率由（5.01±0.75）％上升至（13.11±0.89）％（P＜0.01），Notch1及Oct4錶達明顯下調（均 P＜0.01）；Capan‐2細胞中與單純使用吉西他濱比較，聯閤使用薑黃素組細胞活力由（47.13±2.39）％下降至（5.36±0.25）％（ P＜0.01），細胞凋亡率由（13.02±1.79）％上升至（20.11±1.11）％（P＜0.01），而Notch1及Oct4錶達未見明顯差異；PANC‐1細胞中與單純使用吉西他濱比聯閤使用薑黃素組細胞活力由（82.59±5.69）％下降至（7.33±0.25）％（ P＜0.01），細胞凋亡率由（6.77±2.03）％上升至（25.08±3.14）％（P＜0.01），Notch1及Oct4錶達明顯下調（P＜0.05）。結論薑黃素可協同吉西他濱抑製胰腺癌細胞的增殖、誘導細胞的凋亡及抑製腫瘤榦細胞錶型特徵。
목적：연구연합사용강황소급길서타빈대이선암세포증식、조망급종류간세포표형특정적영향，초보평개강황소치료이선암적림상응용전경。방법채용악성정도、분화정도불동적이선암세포계panc03.27、Capan‐2급PANC‐1세포，분위DM SO처리조、단순길서타빈처리조、단순강황소처리조이급길서타빈연합강황소처리조，채용CCK8（Cell Counting Kit‐8）시제합측정세포증식력적개변；동시채용FITC Annexin V조망검측시제합분석불동처리조지간세포조망솔적차이，이용Western blot검측이선암간성기인Notch1급Oct4적표체개변。결과 Panc03.27세포중여단순사용길서타빈비교，연합사용강황소조세포활력유（46.70±3.49）％하강위（35.53±1.84）％（ P＝0.047），세포조망솔유（5.01±0.75）％상승지（13.11±0.89）％（P＜0.01），Notch1급Oct4표체명현하조（균 P＜0.01）；Capan‐2세포중여단순사용길서타빈비교，연합사용강황소조세포활력유（47.13±2.39）％하강지（5.36±0.25）％（ P＜0.01），세포조망솔유（13.02±1.79）％상승지（20.11±1.11）％（P＜0.01），이Notch1급Oct4표체미견명현차이；PANC‐1세포중여단순사용길서타빈비연합사용강황소조세포활력유（82.59±5.69）％하강지（7.33±0.25）％（ P＜0.01），세포조망솔유（6.77±2.03）％상승지（25.08±3.14）％（P＜0.01），Notch1급Oct4표체명현하조（P＜0.05）。결론강황소가협동길서타빈억제이선암세포적증식、유도세포적조망급억제종류간세포표형특정。
Objective To investigate the effect of combination therapy with curcumin and gemcitabine on the proliferation , apoptosis and cancer stem cell phenotype of pancreatic cancer cells in order to preliminarily examine the application potential of curcumin to the treatment of pancreatic cancer.Methods Human pancreatic cancer cell lines of different malignancy and differ‐entiation ,panc03.27 ,Capan‐2 and PANC‐1 ,were used in this study.They were divided into DMSO group ,gemcitabine treat‐ment group ,curcumin treatment group and gemcitabine+curcumin treatment group in terms of different treatments.Cell count‐ing Kit‐8 assay and flow cytometry were employed to detect the proliferation activity and apoptosis rate of these pancreatic canc‐er cells ,respectively.The protein expression of genes associated with cancer stem cells (Notch1 and Oct4)was measured by Western blot.Results Comparison of the proliferation and apoptosis between the single gemcitabine group and the combination group found that ,in panc03.27 cells ,the cell viability was reduced from(46.70 ± 3.49)% to(35.53 ± 1.84)% (P=0.047) ,the apoptosis rate was increased from(5.01 ± 0.75)% to(13.11 ± 0.89)% (P<0.01)and the expression levels of Notch1 and Oct4 were suppressed(P< 0.01);in Capan‐2 cells ,the cell viability was reduced from(47.13 ± 2.39)% to(5.36 ± 0.25)% (P<0.01) ,the apoptosis rate was increased from(13.02 ± 1.79)% to(20.11 ± 1.11)% (P< 0.01) ,and the expression levels of Notch1 and Oct4 were not significantly different;in PANC‐1 cells ,the cell viability was reduced from(82.59 ± 5.69)% to(7.33 ± 0.25)% (P<0.01) ,the apoptosis rate was increased from(6.77 ± 2.03)% to(25.08 ± 3.14)% (P<0.01) ,and the expres‐sion levels of Notch1 and Oct4 were suppressed(P<0.05).Conclusion Curcumin can synergistically inhibit the proliferation , promote the apoptosis of pancreatic cancer cells ,and suppress the phenotype of cancer stem cells when used with gemcitabine.
