CAJ | 학술논문

目的观察大鼠肝缺血再灌注损伤模型肺组织的氧化应激状态以及抗氧化酶过氧化氢酶(CAT)、超氧化物歧化酶(SOD)的表达变化,探讨肝缺血再灌注损伤对肺组织的影响和CAT、SOD的抗氧化作用.方法采用无损伤血管夹夹闭通往大鼠肝右叶、肝中叶的血管和胆管30 min,然后松开血管夹恢复肝脏血液供应,制造大鼠70％肝脏缺血再灌注损伤模型;再灌注6h后取血、肝脏和肺.血清丙氨酸氨基转移酶(ALT)活性采用速率法测定.采用HE染色法观察肝脏的形态学改变.肺组织丙二醛(MDA)含量采用硫代巴比妥酸比色法测定,抗氧化酶CAT、SOD的mRNA表达水平采用RT-PCR的方法测定,蛋白水平的表达变化采用Western blot法测定.结果与对照组相比,肝脏缺血再灌注损伤组大鼠血清ALT活性和肺组织内MDA的含量均明显升高,HE染色结果显示肝组织结构受损明显,肺组织抗氧化酶CAT、SOD的mRNA水平和蛋白水平也均明显升高.结论肝缺血再灌注损伤使肺组织也遭受过氧化损伤;抗氧化酶CAT、SOD在此过程中可能发挥了抗氧化应激作用,对肺脏具有保护作用.
목적 관찰대서간결혈재관주손상모형폐조직적양화응격상태이급항양화매과양화경매(CAT)、초양화물기화매(SOD)적표체변화,탐토간결혈재관주손상대폐조직적영향화CAT、SOD적항양화작용.방법 채용무손상혈관협협폐통왕대서간우협、간중협적혈관화담관30 min,연후송개혈관협회복간장혈액공응,제조대서70％간장결혈재관주손상모형;재관주6h후취혈、간장화폐.혈청병안산안기전이매(ALT)활성채용속솔법측정.채용HE염색법관찰간장적형태학개변.폐조직병이철(MDA)함량채용류대파비타산비색법측정,항양화매CAT、SOD적mRNA표체수평채용RT-PCR적방법측정,단백수평적표체변화채용Western blot법측정.결과 여대조조상비,간장결혈재관주손상조대서혈청ALT활성화폐조직내MDA적함량균명현승고,HE염색결과현시간조직결구수손명현,폐조직항양화매CAT、SOD적mRNA수평화단백수평야균명현승고.결론 간결혈재관주손상사폐조직야조수과양화손상;항양화매CAT、SOD재차과정중가능발휘료항양화응격작용,대폐장구유보호작용.
Objective To observe the oxidative stress level of lung tissue and expression change of catalase (CAT) and superoxide dismutase (SOD) in hepatic ischemia-reperfusion injury (HIRI) model of rats, to explore the effect of HIRI on lung tissue and antioxidant function of CAT and SOD.Methods The HIRI model of rats was established by clipping the hepatic blood vessel both hepatic right and middle lobes as well as bile duct with non-damage vascular clamp for 30 mins.The serum, hepatic tissues and lung were taken followed by six hours clipping.The activity of serum alanine aminotransferase (ALT) was detected with rate method.The morphological changes of liver were observed with HE staining.The malonaldehyde (MDA) content in lung tissue was determined with thiobarbituric acid colorimetric method.The expressions of CAT and SOD mRNA in lung tissue were evaluated by RT-PCR and protein levels of CAT and SOD were estimated by Western blot.Results Compared with the control group,the activity of ALT in serum,MDA content in lung tissue of HIRI rats were significantly increased.Results of HE staining showed that structure of liver tissue of HIRI model was significantly impaired.The mRNA and protein levels of CAT and SOD in lung tissue of HIRI model were also increased significantly.Conclusions HIRI can also cause the peroxidative damage of lung tissue.The antioxidase CAT and SOD may play an important role in inhibiting the oxidative-stress response during the process and have the protecting function for lung tissue.