国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
International Journal of Respiration
2015年
19期
1483-1487
,共5页
张春玲%张利娟%董福实%宋琪%胡晓晨%鲁犇%张一梅
張春玲%張利娟%董福實%宋琪%鬍曉晨%魯犇%張一梅
장춘령%장리연%동복실%송기%호효신%로분%장일매
肺血栓栓塞%组织因子%组织因子途径抑制物%白介素18%白介素10
肺血栓栓塞%組織因子%組織因子途徑抑製物%白介素18%白介素10
폐혈전전새%조직인자%조직인자도경억제물%백개소18%백개소10
Pulmonary thromboembolism%Tissue factor%Tissue factor pathway inhibitor%Interleukin-18%Interleukin-10
目的 通过建立大鼠肺血栓栓塞(pulmonary thromboembolism,PTE)模型,观察不同阶段肺组织中组织因子(tissue factor,TF)、组织因子途径抑制物(tissue factor pathway inhibitor,TFPI)、IL-18、IL-10的动态变化,研究TF、TFPI、IL-18、IL-10在PTE中的作用,探讨其之间的相互关系.方法 健康雄性大鼠35只,随机分为5组,每组7只:对照组、栓塞1d组、栓塞3d组、栓塞8d组、栓塞14 d组;将栓子经颈静脉注入,并在第14天进行第2次栓塞,制备PTE模型;在不同时间处死大鼠,取出肺组织;HE染色观察肺组织的病理变化;采用免疫组织化学方法分别检测TF、TFPI、IL-18、IL-10在肺组织表达情况.结果 实验组TF、TFPI、IL-18、IL-10第1天较对照组显著升高(P＜0.05),第3天、第8天逐渐下降,第14天较第1天升高(P＜0.05).实验组TFPI与TF的表达呈直线正相关(r=0.84,P＜0.000 1);实验组IL-10与IL-18的表达呈直线正相关(r=0.74,P＜0.000 1);实验组IL-18与TF的表达呈直线正相关(r =0.68,P＜0.000 1).结论 PTE同时存在凝血活性及炎症反应增强,凝血与炎症反应密切相关;栓塞初期机体TF、TFPI、IL-18、IL-10均被激活;实验组不同时间段TF、TFPI、IL-18、IL-10的表达是变化的,并且与病情平行,可作为PTE病情评估的参考指标.
目的 通過建立大鼠肺血栓栓塞(pulmonary thromboembolism,PTE)模型,觀察不同階段肺組織中組織因子(tissue factor,TF)、組織因子途徑抑製物(tissue factor pathway inhibitor,TFPI)、IL-18、IL-10的動態變化,研究TF、TFPI、IL-18、IL-10在PTE中的作用,探討其之間的相互關繫.方法 健康雄性大鼠35隻,隨機分為5組,每組7隻:對照組、栓塞1d組、栓塞3d組、栓塞8d組、栓塞14 d組;將栓子經頸靜脈註入,併在第14天進行第2次栓塞,製備PTE模型;在不同時間處死大鼠,取齣肺組織;HE染色觀察肺組織的病理變化;採用免疫組織化學方法分彆檢測TF、TFPI、IL-18、IL-10在肺組織錶達情況.結果 實驗組TF、TFPI、IL-18、IL-10第1天較對照組顯著升高(P＜0.05),第3天、第8天逐漸下降,第14天較第1天升高(P＜0.05).實驗組TFPI與TF的錶達呈直線正相關(r=0.84,P＜0.000 1);實驗組IL-10與IL-18的錶達呈直線正相關(r=0.74,P＜0.000 1);實驗組IL-18與TF的錶達呈直線正相關(r =0.68,P＜0.000 1).結論 PTE同時存在凝血活性及炎癥反應增彊,凝血與炎癥反應密切相關;栓塞初期機體TF、TFPI、IL-18、IL-10均被激活;實驗組不同時間段TF、TFPI、IL-18、IL-10的錶達是變化的,併且與病情平行,可作為PTE病情評估的參攷指標.
목적 통과건립대서폐혈전전새(pulmonary thromboembolism,PTE)모형,관찰불동계단폐조직중조직인자(tissue factor,TF)、조직인자도경억제물(tissue factor pathway inhibitor,TFPI)、IL-18、IL-10적동태변화,연구TF、TFPI、IL-18、IL-10재PTE중적작용,탐토기지간적상호관계.방법 건강웅성대서35지,수궤분위5조,매조7지:대조조、전새1d조、전새3d조、전새8d조、전새14 d조;장전자경경정맥주입,병재제14천진행제2차전새,제비PTE모형;재불동시간처사대서,취출폐조직;HE염색관찰폐조직적병리변화;채용면역조직화학방법분별검측TF、TFPI、IL-18、IL-10재폐조직표체정황.결과 실험조TF、TFPI、IL-18、IL-10제1천교대조조현저승고(P＜0.05),제3천、제8천축점하강,제14천교제1천승고(P＜0.05).실험조TFPI여TF적표체정직선정상관(r=0.84,P＜0.000 1);실험조IL-10여IL-18적표체정직선정상관(r=0.74,P＜0.000 1);실험조IL-18여TF적표체정직선정상관(r =0.68,P＜0.000 1).결론 PTE동시존재응혈활성급염증반응증강,응혈여염증반응밀절상관;전새초기궤체TF、TFPI、IL-18、IL-10균피격활;실험조불동시간단TF、TFPI、IL-18、IL-10적표체시변화적,병차여병정평행,가작위PTE병정평고적삼고지표.
Objective To explore the change and relationship of tissue factor (TF),tissue factor pathway inhibitor (TFPI),interleukin-18 (IL-18),and interleukin-10 (IL-10) at different stages in lung tissue of rat model with pulmonary thromboembolism (PTE).Methods 35 male rats were randomly divided into five groups:embolism 1-day, 3-day,8-day, 14-day and control group.The rats in embolism 14-day group were received embolus injection again at the 14th day.The rats were sacrificed at different time and lung tissues were kept for testing.The expressions of TF,TFPI,IL-18,and IL-10 in lung tissue were tested by immunohistochemistry.Results The expressions of TF, TFPI, IL-18, and IL-10 in rats of embolism 1-day group were higher than those in control group (P ＜0.05) ,and decreased at the 3rd day and the 8th day after embolism gradually, and those at the 14th day after embolism were higher than those at the 1st day after embolism (P ＜0.05).In the PTE group, the expression of TFPI was positively associated with TF (r =0.84, P ＜0.000 1), IL-10 was positively related to IL-18 (r =0.74, P ＜ 0.000 1), and IL-18 was positively linked to TF (r =0.68, P ＜ 0.000 1).Conclusions There are hypercoagulability activity and high inflammatory response state in PTE, and inflammation and coagulation system are closely associated with each other.They may be reference indexes for the assessment of severity of PTE.
