CAJ | 학술논문

目的研究热休克蛋白70(HSPs70)mRNA表达及蛋白水平对职业性锰接触者的神经保护作用.方法于2008—2009年,采用横断面研究设计,应用分层随机抽样方法,在山东省某机车车辆厂选择接触锰浓度稳定、作业位置相对固定、工作1年以上的专职电焊作业人员作为暴露组,共180名;采用上述方法选择与暴露组民族、性别、年龄、出生地相似,最近3年内无锰职业接触的同机车车辆厂非电焊工人员作为对照组,共100名.以SYBR Green I嵌合Real-time PCR检测HSPs70 mRNA含量表达;应用Western blot法检测HSPs70的蛋白含量;并应用火焰原子吸收法测定锰职业接触工人的空气锰个体接触剂量.用8 h时间加权平均浓度(8h-TWA)表示锰个体接触浓度,根据空气锰工作场所职业接触限值(8h-TWA=0.15 mg/m3) ,将暴露组分为高暴露组(>0.15 mg/m3)和低暴露组(<0.15 mg/m3)结果暴露组个体锰接触剂量为(0.25±0.31)mg/m3,高于对照组[(0.06±0.02)mg/m3](t=6.15,P=0.001);高暴露组个体锰接触剂量为(0.42±0.34)mg/m3,高于低暴露组[(0.09±0.07)mg/m3](t=9.80,P=0.001).暴露组HSPs70 mRNA及蛋白表达量分别为5.65 ± 0.21、3.26 ± 0.15,均高于对照组(0.41±0.03和1.32±0.12)(t值分别为18.91、8.68,P值均为0.001);高暴露组的HSPs70 mRNA及蛋白表达水平分别为6.48 ± 0.37、3.67 ± 0.26,亦均高于低暴露组(5.15 ± 0.23和3.02 ± 0.19)(t值分别为3.24、2.04,P值分别为0.003、0.043).结论锰接触者外周血淋巴细胞HSPs70水平及HSPs70 mRNA表达的增高与保护神经细胞免受锰刺激引起的脂质过氧化等损伤有关.
목적 연구열휴극단백70(HSPs70)mRNA표체급단백수평대직업성맹접촉자적신경보호작용.방법 우2008—2009년,채용횡단면연구설계,응용분층수궤추양방법,재산동성모궤차차량엄선택접촉맹농도은정、작업위치상대고정、공작1년이상적전직전한작업인원작위폭로조,공180명;채용상술방법선택여폭로조민족、성별、년령、출생지상사,최근3년내무맹직업접촉적동궤차차량엄비전한공인원작위대조조,공100명.이SYBR Green I감합Real-time PCR검측HSPs70 mRNA함량표체;응용Western blot법검측HSPs70적단백함량;병응용화염원자흡수법측정맹직업접촉공인적공기맹개체접촉제량.용8 h시간가권평균농도(8h-TWA)표시맹개체접촉농도,근거공기맹공작장소직업접촉한치(8h-TWA=0.15 mg/m3) ,장폭로조분위고폭로조(>0.15 mg/m3)화저폭로조(<0.15 mg/m3)결과 폭로조개체맹접촉제량위(0.25±0.31)mg/m3,고우대조조[(0.06±0.02)mg/m3](t=6.15,P=0.001);고폭로조개체맹접촉제량위(0.42±0.34)mg/m3,고우저폭로조[(0.09±0.07)mg/m3](t=9.80,P=0.001).폭로조HSPs70 mRNA급단백표체량분별위5.65 ± 0.21、3.26 ± 0.15,균고우대조조(0.41±0.03화1.32±0.12)(t치분별위18.91、8.68,P치균위0.001);고폭로조적HSPs70 mRNA급단백표체수평분별위6.48 ± 0.37、3.67 ± 0.26,역균고우저폭로조(5.15 ± 0.23화3.02 ± 0.19)(t치분별위3.24、2.04,P치분별위0.003、0.043).결론 맹접촉자외주혈림파세포HSPs70수평급HSPs70 mRNA표체적증고여보호신경세포면수맹자격인기적지질과양화등손상유관.
Objective To analyze the expression levels of heat shock protein70 (HSPs70) and HSPs70 mRNA in different exposure to manganese, and research the neuroprotective effect on the career exposure to manganese. Methods From 2008 to 2009,with cross-sectional study design, and in a locomotive and rolling stock works, by stratified random sampling method, the exposed sample consisted of 180 welders from different welding shops and 100 unexposed in the last three years, non-welder controls with age-matched workers of similar socioeconomic status from the same industry. The control workers had not been exposed to neurotoxic chemicals. The mRNA expressions of four different metabolic enzyme were detected by SYBR Green I quantitative real-time polymerase chain reaction. The expression levels of the two enzymes mRNA in different exposure to manganese were analyzed. The expressions of HSPs70 were detected by Western blot. The concentration of air manganese was determined by GFAAS.The average concentration of 8 h time (8h-TWA) was used to express the level of individual exposure to manganese, according to the air manganese workplace occupational exposure limit (8h-TWA=0.15 mg/m3), the exposed group is divided into high exposed group (>0.15 mg/m3) and low exposure group (<0.15 mg/m3). Results The individuals exposed to manganese dose of exposed group ((0.25 ± 0.31) mg/m3) was higher than the control group ((0.06 ± 0.02) mg/m3) (t=6.15, P=0.001); individuals exposed to manganese dose of high exposure group for (0.42±0.34) mg/m3, which was higher than low exposure group (0.09±0.07) mg/m3 (t=9.80, P=0.001). HSPs70 mRNA and protein of exposure group (5.65 ± 0.21, 3.26 ± 0.15) were higher than the reference group (0.41 ± 0.03, 1.32 ± 0.12) (t=18.91, t=8.68, P=0.001). HSP70 mRNA and protein of high exposure group(6.48±0.37, 3.67±0.26) were higher than the low exposure group (5.15±0.23, 3.02±0.19)(t=3.24,t=2.01,P=0.003, P=0.043). Conclusion The expression of peripheral blood lymphocytes HSPs70 level and HSPs70 mRNA workers exposed to manganese increased and protect nerve cells from related to Mn stimulation induced lipid peroxidation damag.