中华预防医学杂志
中華預防醫學雜誌
중화예방의학잡지
Chinese Journal of Preventive Medicine
2015年
10期
892-895
,共4页
杜燕华%张白帆%李懿%马红霞%黄学勇%许汴利
杜燕華%張白帆%李懿%馬紅霞%黃學勇%許汴利
두연화%장백범%리의%마홍하%황학용%허변리
登革热病毒%基因组%序列进化分析
登革熱病毒%基因組%序列進化分析
등혁열병독%기인조%서렬진화분석
Dengue virus%Genome%Phylogenetic analysis
目的 对河南省登革热输入性病例进行实验室诊断,测定全基因序列,分析序列特征和传播来源.方法 河南省禹州市报告1例登革热疑似病例,该病例2014年9月19日从广东省佛山市返回,经流行病学调查,采集血清标本,进行登革热实验室诊断,用Vero细胞分离培养病毒株,合成引物分段扩增,测序后拼接获得全基因组序列,再用生物学软件进行序列分析.结果 该病例实验室确诊为登革热1型感染,血清标本在Vero细胞上分离培养成功,测序后拼接成全长10 670 nt的全基因组序列,经系统进化分析,属于登革热1型病毒第Ⅴ基因亚型,与印度2008—2011年分离病毒株亲缘关系最近,核酸同源性在98.2%~99.4%之间,未发生重组.结论 河南省首次发现登革热1型输入性病例,结合该病例发病前曾去过广东的流行病学调查资料,推测印度毒株之前已传入广东省,此次又输入河南省.
目的 對河南省登革熱輸入性病例進行實驗室診斷,測定全基因序列,分析序列特徵和傳播來源.方法 河南省禹州市報告1例登革熱疑似病例,該病例2014年9月19日從廣東省彿山市返迴,經流行病學調查,採集血清標本,進行登革熱實驗室診斷,用Vero細胞分離培養病毒株,閤成引物分段擴增,測序後拼接穫得全基因組序列,再用生物學軟件進行序列分析.結果 該病例實驗室確診為登革熱1型感染,血清標本在Vero細胞上分離培養成功,測序後拼接成全長10 670 nt的全基因組序列,經繫統進化分析,屬于登革熱1型病毒第Ⅴ基因亞型,與印度2008—2011年分離病毒株親緣關繫最近,覈痠同源性在98.2%~99.4%之間,未髮生重組.結論 河南省首次髮現登革熱1型輸入性病例,結閤該病例髮病前曾去過廣東的流行病學調查資料,推測印度毒株之前已傳入廣東省,此次又輸入河南省.
목적 대하남성등혁열수입성병례진행실험실진단,측정전기인서렬,분석서렬특정화전파래원.방법 하남성우주시보고1례등혁열의사병례,해병례2014년9월19일종광동성불산시반회,경류행병학조사,채집혈청표본,진행등혁열실험실진단,용Vero세포분리배양병독주,합성인물분단확증,측서후병접획득전기인조서렬,재용생물학연건진행서렬분석.결과 해병례실험실학진위등혁열1형감염,혈청표본재Vero세포상분리배양성공,측서후병접성전장10 670 nt적전기인조서렬,경계통진화분석,속우등혁열1형병독제Ⅴ기인아형,여인도2008—2011년분리병독주친연관계최근,핵산동원성재98.2%~99.4%지간,미발생중조.결론 하남성수차발현등혁열1형수입성병례,결합해병례발병전증거과엄동적류행병학조사자료,추측인도독주지전이전입광동성,차차우수입하남성.
Objective To diagnose imported dengue fever case from Henan province, and to sequence and analyze the characteristics of whole genome sequence, and to explore the possible viral origin source. Methods A suspected dengue fever case was reported in Yuzhou city, Henan province. The patient returned from foshan, Guangdong province on September 19, 2014, after the epidemiological investigation and serum specimen collected, which dengue fever case was diagnosed in the laboratory, then it was inoculated on Vero cells. Whole genome sequence was amplified by several pairs primers and characterized using biologic software. Results The imported case was diagnosed as dengue virus 1 serotype infection. Dengue 1 strain was isolated using Vero cells successfully. Whole genome was 10 670 nt, which belonged to dengue virus 1 serotype Ⅴ genotype and didn't found any recombination event. The phylogenetic analysis demonstrated that the strain was closed to Indian starins isolated in 2008-2011, and the homology of nucleotide sequence was between 98.2%-99.4%. Conclusion It was the first time to discover imported dengue 1 serotype case in Henan province. However, according to the patient has been to Guangdong province before onset, it inferred that the Indian strain had been imported to Guangdong province before this case in Henan province.
