CAJ | 학술논문

目的观察NVP-BKM120(BKM120)对三阴性乳腺癌细胞系的作用效果,分析BKM120对三阴性乳腺癌细胞系干细胞的杀伤效果.方法将乳腺癌细胞系MDA-MB-231、Cal51分为对照组和实验组,通过MTT法、体外克隆形成实验、球囊形成抑制实验、免疫蛋白沉淀实验以及体内干细胞移植瘤实验检测分析BKM120对三阴性乳腺癌非干细胞及干细胞的效果.结果 BKM120对MDA-MB-231非干细胞及干细胞IC50值分别为(3.07 ±0.14)μmol/L、(20.01 ±3.46)μmol/L.BKM120显著抑制三阴性乳腺癌细胞的生长、体外克隆以及微球体形成.在不引起明显毒副反应的前提下, BKM120能够有效抑制裸鼠移植瘤生长.结论 BKM120能够有效杀伤三阴性乳腺癌细胞.
목적 관찰NVP-BKM120(BKM120)대삼음성유선암세포계적작용효과,분석BKM120대삼음성유선암세포계간세포적살상효과.방법 장유선암세포계MDA-MB-231、Cal51분위대조조화실험조,통과MTT법、체외극륭형성실험、구낭형성억제실험、면역단백침정실험이급체내간세포이식류실험검측분석BKM120대삼음성유선암비간세포급간세포적효과.결과 BKM120대MDA-MB-231비간세포급간세포IC50치분별위(3.07 ±0.14)μmol/L、(20.01 ±3.46)μmol/L.BKM120현저억제삼음성유선암세포적생장、체외극륭이급미구체형성.재불인기명현독부반응적전제하, BKM120능구유효억제라서이식류생장.결론 BKM120능구유효살상삼음성유선암세포.
Objective To investigatethe effect of NVP-BKM120 on the triple-negative breast cancer cell lines.Methods Breast cancer cell line MDA-MB-231 and Cal51 were divided into control group and experimental group.The inhibitory effects of BKM120 were evaluated by MTT assays.The drug effects on CSC population and characteristics were investigated through mammosphere formation assay and colony formation assay.Western blot was used to observe the expressionof related protein.The BALB/c mice were injected with stem cells (SCs) and different treatments were administered subsequently.Results In MDA-MB-231 cell lines, IC50 of BKM120 was (20.01 ±3.46)μmol/L for SCs and(3.07 ±0.14)μmol/L for total cells.BKM120 significantly inhibit the cell growth ,in vitrocloning and microspheres formed of triple-negative breast cancer cells.BKM120 can inhibit tumor growth in nude mice without significant adverse reactions.Conclusion BKM120 can significantly inhibit the proliferation of the triple-negative breast cancer cell lines.