CAJ | 학술논문

目的本文从高气压培养环境对胃癌细胞PHD3基因表达的影响入手,进而探讨压力对肿瘤细胞分化的影响.方法本研究设计并研制了"调压式培养箱",用于对培养的细胞施加持续性静压力或间歇性静压力.在实验中把1640培养液培养的胃癌SGC7901细胞分别在正常气压、加压760 mmHg和加压1520 mmHg的条件下,37℃培养30 min.通过RT-PCR和Western印迹方法检测了胃癌SGC7901细胞中PHD3基因和蛋白表达的变化.结果实验结果证明,本研究设计并研制的调压式培养系统能够完成设计所需实验,通过调节空气和二氧化碳的比例,可以有效地维持细胞培养液的pH值不发生显著变化(P=0.6213).通过将正常气压组、加压760 mmHg组和加压1520 mmHg组的实验结果经过统计学分析对比,发现高气压培养环境能够抑制PHD3蛋白的表达(P=0.0087).结论本研究显示高气压培养环境能够抑制PHD3蛋白的表达,从而可能抑制SGC7901细胞的分化.
목적 본문종고기압배양배경대위암세포PHD3기인표체적영향입수,진이탐토압력대종류세포분화적영향.방법 본연구설계병연제료"조압식배양상",용우대배양적세포시가지속성정압력혹간헐성정압력.재실험중파1640배양액배양적위암SGC7901세포분별재정상기압、가압760 mmHg화가압1520 mmHg적조건하,37℃배양30 min.통과RT-PCR화Western인적방법검측료위암SGC7901세포중PHD3기인화단백표체적변화.결과 실험결과증명,본연구설계병연제적조압식배양계통능구완성설계소수실험,통과조절공기화이양화탄적비례,가이유효지유지세포배양액적pH치불발생현저변화(P=0.6213).통과장정상기압조、가압760 mmHg조화가압1520 mmHg조적실험결과경과통계학분석대비,발현고기압배양배경능구억제PHD3단백적표체(P=0.0087).결론 본연구현시고기압배양배경능구억제PHD3단백적표체,종이가능억제SGC7901세포적분화.
Objective To evaluate effects of high pressure on PHD3 expression and differentiation of cancer cells.Methods To increase the extracellular pressure, we designed and developed a pressure adjustable incubator, which could maintain not only the pHof culture solution by regulating the ratio of air and carbon dioxide (CO2) but also the temperature through heating system.In this study, SGC7901 cells were incubated in RPMI 1640 and divided into three groups.After incubation for 30 minutes at 37 ℃ under ambient (760 mmHg) and increased pressure (1520 mmHg) conditions, cells were harvested for detection of PHD3 and β-actin expression by Western blot or RT-PCR.Results By RT-PCR and Western blot, we found that the expression of pHD3 decreased significantly.Conclusion In conclusion, high extracellular pressure suppresses expression of PHD3 and may inhibit SGC7901 cells differentiation.