CAJ | 학술논문

目的研究结直肠癌演变过程中Oct-4表达的变化,探讨Oct-4的表达与结直肠癌患者预后的相关性.方法收集结直肠癌、配对的远端正常肠组织、结肠息肉组织,定量PCR(qRT-PCR)、流式细胞术(FCM)、免疫组织化学(IHC)检测不同组织中Oct-4表达水平.收集结直肠癌患者临床资料,统计学分析Oct-4的表达与临床参数的相关性.Kaplan-Meier曲线法分析Oct-4的表达与患者生存期的相关性.结果 qRT-PCR结果显示,Oct-4 mRNA在正常组织、结肠息肉组织和恶性结直肠组织的相对表达量分别为(0.23±0.16) ×10-5、(1.19±0.76)×10-5、(3.79±1.94) ×10-5,组间差异具有统计学意义(F=8.633,P=0.001).IHC检测正常组织、结肠息肉组织和恶性结直肠组织Oct-4阳性率分别为4.43％、12.68％、40.51％,差异具有统计学意义(x2=66.311,P＜0.001).Oct-4的表达与结直肠癌的病理分期(x2=7.248,P=0.007)、淋巴结转移(x2 =4.888,P=0.027)、远处转移(x2=5.732,P=0.017)、TNM分期(x2=4.174,P=0.041)相关.Kaplan-Meier生存曲线分析显示,Oct-4表达阳性患者的中位生存期(37.0个月)显著短于阴性患者(76.0个月),差异具有统计学意义(x2=14.050,P=0.001).结论 Oct-4的表达在结直肠癌演变过程中逐步升高,Oct-4的异常表达可能在结直肠癌演变过程中发挥一定作用,有望作为结直肠癌临床诊断、病情评估、预后判断的重要标志分子.
목적 연구결직장암연변과정중Oct-4표체적변화,탐토Oct-4적표체여결직장암환자예후적상관성.방법 수집결직장암、배대적원단정상장조직、결장식육조직,정량PCR(qRT-PCR)、류식세포술(FCM)、면역조직화학(IHC)검측불동조직중Oct-4표체수평.수집결직장암환자림상자료,통계학분석Oct-4적표체여림상삼수적상관성.Kaplan-Meier곡선법분석Oct-4적표체여환자생존기적상관성.결과 qRT-PCR결과현시,Oct-4 mRNA재정상조직、결장식육조직화악성결직장조직적상대표체량분별위(0.23±0.16) ×10-5、(1.19±0.76)×10-5、(3.79±1.94) ×10-5,조간차이구유통계학의의(F=8.633,P=0.001).IHC검측정상조직、결장식육조직화악성결직장조직Oct-4양성솔분별위4.43％、12.68％、40.51％,차이구유통계학의의(x2=66.311,P＜0.001).Oct-4적표체여결직장암적병리분기(x2=7.248,P=0.007)、림파결전이(x2 =4.888,P=0.027)、원처전이(x2=5.732,P=0.017)、TNM분기(x2=4.174,P=0.041)상관.Kaplan-Meier생존곡선분석현시,Oct-4표체양성환자적중위생존기(37.0개월)현저단우음성환자(76.0개월),차이구유통계학의의(x2=14.050,P=0.001).결론 Oct-4적표체재결직장암연변과정중축보승고,Oct-4적이상표체가능재결직장암연변과정중발휘일정작용,유망작위결직장암림상진단、병정평고、예후판단적중요표지분자.
Objective To study dynamic expression of Oct-4 during development of colorectal cancer (CRC) and investigate the association between Oct-4 expression and prognosis of patients with CRC.Methods CRC tissues, matched non-tumor tissues and colonic polyp tissues were collected and Oct-4 expressions were analyzed by quantitative reverse transcription-polymerase chain reaction (qRT-PCR), flow cytometry analysis (FCM) and immunohistochemistry (IHC).Medical records of patients with CRC were reviewed.Clinicopathological analysis was performed to assess the association between Oct-4 expression and certain chnicopathological parameters.Kaplane-Meier survival curve was conducted to evaluate association between Oct-4 expression and survival time of patients with CRC.Results The results of qRT-PCR showed that the relative expressions of Oct-4 mRNA in matched non-tumor, colonic polyp and CRC tissues were (0.23 ±0.16) × 10-5, (1.19 ± 0.76) × 10-5 and (3.79 ± 1.94) × 10-5 respectively, with a significant difference (F =8.633, P =0.001).IHC analysis displayed that the positive expression ratios of Oct-4 in matched non-tumor tissues, colonic polyp tissues and CRC tissues were 4.43％, 12.68％ and 40.51％ respectively, and a significant difference was discovered among them (x2 =66.311, P ＜ 0.001).Oct-4 expre-ssion in CRC was significantly correlated with pathological stage (x2 =7.248, P =0.007), lymph node metastasis (x2 =4.888, P =0.027), distant metastasis (x2 =5.732, P=0.017) and TNM stage (x2 =4.174, P=0.041).Kaplan-Meier survival curve analysis demonstrated that Oct-4 positive cases had a shorter median survival time (37.0 months) compared with Oct-4 negative cases (76.0 months), with a significant difference (x2 =14.050, P =0.001).Conclusion The expression of Oct-4 is step-wise increased during development of CRC.The abnormal expression of Oct-4 may play certain roles in the development of CRC.Oct-4 may be an important biomarker for the clinical diagnosis, assessment of disease and prognosis in CRC.